The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Current methods for the isolation of extracellular vesicles
Open accessAbstract
Extracellular vesicles (EVs), including microvesicles and exosomes, are nano- to micron-sized vesicles, which may deliver bioactive cargos that include lipids, growth factors and their receptors, proteases, signaling molecules, as well as mRNA and non-coding RNA, released from the cell of origin, to target cells. EVs are released by all cell types and likely induced by mechanisms involved in oncogenic transformation, environmental stimulation, cellular activation, oxidative stress, or death. Ongoing studies investigate the molecular mechanisms and mediators of EVs-based intercellular communication at physiological and oncogenic conditions with the hope of using this information as a possible source for explaining physiological processes in addition to using them as therapeutic targets and disease biomarkers in a variety of diseases. A major limitation in this evolving discipline is the hardship and the lack of standardization for already challenging techniques to isolate EVs. Technical advances have been accomplished in the field of isolation with improving knowledge and emerging novel technologies, including ultracentrifugation, microfluidics, magnetic beads and filtration-based isolation methods. In this review, we will discuss the latest advances in methods of isolation methods and production of clinical grade EVs as well as their advantages and disadvantages, and the justification for their support and the challenges that they encounter.
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Citations
Cites
1 retracted
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RetractedIsolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
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Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET
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Self-citationB lymphocytes secrete antigen-presenting vesicles.
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Cited by
Isolation of Extracellular Vesicles: General Methodologies and Latest Trends
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Methodological Guidelines to Study Extracellular Vesicles
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Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine
Contreras-Naranjo, J. C., Wu, H.-J., & Ugaz, V. M. (2017) · Lab on a Chip · doi:10.1039/c7lc00592j · Cited by 640
Meng, W., He, C., Hao, Y., Wang, L., Li, L., & Zhu, G. (2020) · Drug Delivery · doi:10.1080/10717544.2020.1748758 · Cited by 592
Metadata
- Authors
- Momen-Heravi, F., Balaj, L., Alian, S., Mantel, P.-Y., Halleck, A. E., Trachtenberg, A. J., Soria, C. E., Oquin, S., Bonebreak, C. M., Saracoglu, E., Skog, J., & Kuo, W. P.
- Venue
- bchm
- Publisher
- Walter de Gruyter GmbH
- Published
- 2013-06-14
- Volume
- 394
- Issue
- 10
- Pages
- 1253-1262
- ISSN
- 1431-6730 (ISSN-L)
- 1437-4315
- OpenAlex
- W2027195751
- Cited by
- 578
- References
- 61
- FWCI
- 10.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Momen-Heravi, F., Balaj, L., Alian, S., Mantel, P.-Y., Halleck, A. E., Trachtenberg, A. J., Soria, C. E., Oquin, S., Bonebreak, C. M., Saracoglu, E., Skog, J., & Kuo, W. P. (2013). Current methods for the isolation of extracellular vesicles. bchm. https://doi.org/10.1515/hsz-2013-0141
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
