The DOI matches its Crossref record; 101 of the 101 references it cites were found in a registry or an index.
The Caco-2 cell monolayer: usefulness and limitations
Abstract
BACKGROUND: The Caco-2 monolayer has been used extensively for the high-throughput screening of drug permeability and identification of substrates, inhibitors, and inducers of intestinal transporters, especially P-glycoprotein (P-gp). Traditionally, the Caco-2 monolayer is viewed as a single barrier rather than a polarized cell monolayer consisting of metabolic enzymes that are sandwiched between two membrane barriers with distinctly different transporters. OBJECTIVE: This review addressed the usefulness and limitations of the Caco-2 cell monolayer in drug discovery and mechanistic studies. METHODS: This mini-review covered applications of the Caco-2 monolayer, clarified misconceptions, and critically addressed issues on data interpretation. CONCLUSION: The catenary model extends the usefulness of Caco-2 monolayer and provides proper mechanistic insight and data interpretation.
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Cites
Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989) · Gastroenterology · doi:10.1016/s0016-5085(89)80072-1 · Cited by 2,396
Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers
Hubatsch, I., Ragnarsson, E. G. E., & Artursson, P. (2007) · Nature Protocols · doi:10.1038/nprot.2007.303 · Cited by 1,499
Kim, R. B., Fromm, M. F., Wandel, C., Leake, B., Wood, A. J., Roden, D. M., & Wilkinson, G. R. (1998) · Journal of Clinical Investigation · doi:10.1172/jci1269 · Cited by 1,054
Yee, S. (1997) · Pharmaceutical Research · doi:10.1023/a:1012102522787 · Cited by 1,030
Allen, J. D., van Loevezijn, A., Rademaker-Lakhai, J. M., van der Valk, M., van Tellingen, O., Reid, G., Schellens, J. H. M., Koomen, G., & Schinkel, A. H. (2002) · PubMed · Cited by 704
Rational Use of in Vitro P-glycoprotein Assays in Drug Discovery
Polli, J. W., Wring, S. A., Humphreys, J. E., Huang, L., Morgan, J. B., Webster, L. O., & Serabjit-Singh, C. S. (2001) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)29270-3 · Cited by 662
Caco-2 Cell Monolayers as a Model for Drug Transport Across the Intestinal Mucosa
Hilgers, A. R., Conradi, R. A., & Burton, P. S. (1990) · Pharmaceutical Research · doi:10.1023/a:1015937605100 · Cited by 587
Hilgendorf, C., Ahlin, G., Seithel, A., Artursson, P., Ungell, A.-L., & Karlsson, J. (2007) · Drug Metabolism and Disposition · doi:10.1124/dmd.107.014902 · Cited by 583
Effect of Chitosan on Epithelial Cell Tight Junctions
Smith, J., Wood, E., & Dornish, M. (2004) · Pharmaceutical Research · doi:10.1023/b:pham.0000012150.60180.e3 · Cited by 467
Sun, D., Lennernas, H., Welage, L. S., Barnett, J. L., Landowski, C. P., Foster, D., Fleisher, D., Lee, K.-D., & Amidon, G. L. (2002) · Pharmaceutical Research · doi:10.1023/a:1020483911355 · Cited by 447
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Role of nanoparticle size, shape and surface chemistry in oral drug delivery
Banerjee, A., Qi, J., Gogoi, R., Wong, J., & Mitragotri, S. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.07.051 · Cited by 773
Development and Characterization of a Human and Mouse Intestinal Epithelial Cell Monolayer Platform
Kozuka, K., He, Y., Koo-McCoy, S., Kumaraswamy, P., Nie, B., Shaw, K., Chan, P., Leadbetter, M., He, L., Lewis, J. G., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.10.013 · Cited by 179
Mechanisms of uptake and transport of particulate formulations in the small intestine
Delon, L., Gibson, R. J., Prestidge, C. A., & Thierry, B. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.02.006 · Cited by 64
Permeability and Conjugative Metabolism of Flaxseed Lignans by Caco-2 Human Intestinal Cells
Mukker, J. K., Michel, D., Muir, A. D., Krol, E. S., & Alcorn, J. (2014) · Journal of Natural Products · doi:10.1021/np4004905 · Cited by 29
Metadata
- Authors
- Sun, H., Chow, E. C., Liu, S., Du, Y., & Pang, K. S.
- Venue
- Expert Opinion on Drug Metabolism & Toxicology
- Publisher
- Informa UK Limited
- Published
- 2008-04
- Volume
- 4
- Issue
- 4
- Pages
- 395-411
- ISSN
- 1742-5255 (ISSN-L)
- 1744-7607
- OpenAlex
- W1994470556
- Cited by
- 527
- References
- 101
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sun, H., Chow, E. C., Liu, S., Du, Y., & Pang, K. S. (2008). The Caco-2 cell monolayer: usefulness and limitations. Expert Opinion on Drug Metabolism & Toxicology. https://doi.org/10.1517/17425255.4.4.395
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
