The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Astn2, A Novel Member of the Astrotactin Gene Family, Regulates the Trafficking of ASTN1 during Glial-Guided Neuronal Migration
Open accessAbstract
Glial-guided neuronal migration is a key step in the development of laminar architecture of cortical regions of the mammalian brain. We previously reported that neuronal protein astrotactin (ASTN1) functions as a neuron-glial ligand during CNS glial-guided migration. Here, we identify a new Astn family member, Astn2, that is expressed at high levels in migrating, cerebellar granule neurons, along with Astn1, at developmental stages when glial-guided migration is ongoing. Biochemical and flow cytometry experiments show that ASTN2 forms a complex with ASTN1 and regulates surface expression of ASTN1. Live imaging of Venus-tagged ASTN1 in migrating cerebellar granule cells reveals the intracellular trafficking of ASTN1-Venus, with ASTN1-Venus accumulating in the forward aspect of the leading process where new sites of adhesion will form. Treatment of migrating neurons with Dynasore, a soluble noncompetitive inhibitor of Dynamin, rapidly arrests the migration of immature granule cells in a reversible manner, suggesting the critical importance of receptor trafficking to neuronal locomotion along Bergmann glial fibers in the developing cerebellum. Together, these findings suggest that ASTN2 regulates the levels of ASTN1 in the plasma membrane and that the release of neuronal adhesions to the glial fiber during neuronal locomotion involves the intracellular trafficking of ASTN1.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“A further gene involved in cell adhesion, astrotactin 2 (ASTN2), which we found to be putatively associated with depression is also involved in ADHD aetiology 32 and plays a role in neuronal development in the brain 33.”
From Genome-wide meta-analysis of depression identifies 102 independent variants and highlights the importance of the prefrontal brain regions (2019) · cites this paper as [33]
In the citing paper
“ASTN2 interacts with ASTN1, regulating its expression, thereby playing a role in neuronal-glial adhesion during migration [55].”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cell Migration: Integrating Signals from Front to Back
Ridley, A. J., Schwartz, M. A., Burridge, K., Firtel, R., Ginsberg, M. H., Borisy, G. G., Parsons, J. T., & Horwitz, A. R. (2003) · Science · doi:10.1126/science.1092053 · Cited by 5,109
Dynasore, a Cell-Permeable Inhibitor of Dynamin
Macia, E., Ehrlich, M., Massol, R., Boucrot, E., Brunner, C., & Kirchhausen, T. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.04.002 · Cited by 1,958
Autism genome-wide copy number variation reveals ubiquitin and neuronal genes
Glessner, J. T., Wang, K., Cai, G., Korvatska, O., Kim, C. E., Wood, S., Zhang, H., Estes, A., Brune, C. W., Bradfield, J. P., et al. (2009) · Nature · doi:10.1038/nature07953 · Cited by 1,421
RAKIC, P. T. (1971) · The Journal of Comparative Neurology · doi:10.1002/cne.901410303 · Cited by 1,343
Neuroligin Expressed in Nonneuronal Cells Triggers Presynaptic Development in Contacting Axons
Scheiffele, P., Fan, J., Choih, J., Fetter, R., & Serafini, T. (2000) · Cell · doi:10.1016/s0092-8674(00)80877-6 · Cited by 1,218
Schaeren‐Wiemers, N., & Gerfin-Moser, A. (1993) · Histochemistry and Cell Biology · doi:10.1007/bf00267823 · Cited by 1,151
The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae.
Terbush, D. R., Maurice, T. C., Roth, D., & Novick, P. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb01039.x · Cited by 843
CENTRAL NERVOUS SYSTEM NEURONAL MIGRATION
Hatten, M. E. (1999) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.22.1.511 · Cited by 808
Self-citationSigismund, S., Argenzio, E., Tosoni, D., Cavallaro, E. C., Polo, S., & Fiore, P. P. D. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.06.012 · Cited by 679
Cited by
Sorted by citations
Howard, D. M., Adams, M. J., Clarke, T.-K., Hafferty, J. D., Gibson, J., Shirali, M., Coleman, J. R. I., Hagenaars, S. P., Ward, J., Wigmore, E. M., et al. (2019) · Nature Neuroscience · doi:10.1038/s41593-018-0326-7 · Cited by 2,565
The Autism Spectrum Disorders Working Group of The Psychiatric Genomics Consortium (2017) · Molecular Autism · doi:10.1186/s13229-017-0137-9 · Cited by 587
Zeggini, E. (2012) · The Lancet · doi:10.1016/s0140-6736(12)60681-3 · Cited by 416
Genome-wide meta-analysis identifies new susceptibility loci for migraine
North American Brain Expression Consortium, Anttila, V., UK Brain Expression Consortium, the International Headache Genetics Consortium, Winsvold, B. S., Gormley, P., Kurth, T., Bettella, F., McMahon, G., Kallela, M., et al. (2013) · Nature Genetics · doi:10.1038/ng.2676 · Cited by 410
Methotrexate-Induced Neurotoxicity and Leukoencephalopathy in Childhood Acute Lymphoblastic Leukemia
Bhojwani, D., Sabin, N. D., Pei, D., Yang, J. J., Khan, R. B., Panetta, J. C., Krull, K. R., Inaba, H., Rubnitz, J. E., Metzger, M. L., et al. (2014) · Journal of Clinical Oncology · doi:10.1200/jco.2013.53.0808 · Cited by 400
Metadata
- Authors
- Wilson, P. M., Fryer, R. H., Fang, Y., & Hatten, M. E.
- Affiliations
- Rockefeller University (US)
- Corresponding author
- Hatten, M. E.
- Venue
- Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2010-06-23
- Volume
- 30
- Issue
- 25
- Pages
- 8529-8540
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC2905051
- OpenAlex
- W1996840895
- Cited by
- 169
- References
- 49
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 13 · 2023: 10 · 2022: 15 · 2021: 14 · 2020: 10 · 2019: 8 · 2018: 8 · 2017: 17 · 2016: 9 · 2015: 8 · 2014: 12 · 2013: 13 · 2012: 11
- FWCI
- 3.24
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (T32GM007524, GM07524)
- National Institute of Neurological Disorders and Stroke (R01NS015429, R01NS051778)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- ASTN2, cerebellar granule cells, receptor trafficking, dynamin, dynasore, leading process, cortical lamination, protein complex formation
- MeSH terms
- Animals, Cell Line, Cell Movement, Cells, Cultured, Cerebellum, Flow Cytometry, Glycoproteins, Hydrazones, Immunohistochemistry, Nerve Tissue Proteins, Neuroglia, Neurons, Transfection, Blotting, Northern, In Situ Hybridization, Microscopy, Confocal, Dynamins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
