Temporal patterns of fos expression in the dentate gyrus after spontaneous seizures in a mouse model of temporal lobe epilepsy
Open accessAbstract
Identifying the brain regions and neuronal cell types that become active at the time of spontaneous seizures remains an important challenge for epilepsy research, and the involvement of dentate granule cells in early seizure events continues to be debated. Although Fos expression is commonly used to evaluate patterns of neuronal activation, there have been few studies of Fos localization after spontaneous seizures. Thus, in a pilocarpine model of recurrent seizures in C57BL/6 mice, Fos expression was examined at multiple time points after spontaneous seizures to follow the temporal and spatial patterns of Fos activation. By 15 min after the beginning of a spontaneous behavioral seizure, Fos labeling was evident in dentate granule cells. This labeling was particularly striking because of its wide extent and relatively uniform appearance in the granule cell layer. At later time points, from 30 min to 4 h after a spontaneous seizure, Fos labeling was also detected in interneurons within the dentate gyrus and in widespread regions of the temporal lobe. Interestingly, the timing of Fos activation appeared to differ among different types of GABAergic interneurons in the dentate gyrus, with labeling of parvalbumin neurons along the base of the granule cell layer preceding that of GABA neurons in the molecular layer. The findings in this mouse model are consistent with previous suggestions that spontaneous seizures in temporal lobe epilepsy may result from a periodic breakdown of the normal filter functions of the dentate gyrus and a resulting increase in hypersynchronous activity of dentate granule cells.
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c-fos is an immediate early gene used as a proxy for neuronal activation.
“Mice were perfused 2h after the EPM test to allow for maximal accumulation of the protein products of c-fos, an immediate early gene used as a proxy for neuronal activation, in interneurons (44).”
From Perineuronal nets, inhibitory interneurons and anxiety-related ventral hippocampal neuronal oscillations are altered by early life adversity (2019) · cites this paper as [44]
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- Authors
- Peng, Z., & Houser, C. R.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Houser, Carolyn R.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2005-08-03
- Volume
- 25
- Issue
- 31
- Pages
- 7210-7220
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6725230
- OpenAlex
- W1999508923
- Cited by
- 106
- References
- 56
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 7 · 2021: 7 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 5 · 2013: 11 · 2012: 7
- FWCI
- 4.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs (I01 BX000404)
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke (R01NS046524, NS046524)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- Fos expression, dentate gyrus, dentate gyrus granule cells, temporal lobe epilepsy, spontaneous seizures, pilocarpine-induced seizures, GABAergic interneurons, parvalbumin neurons, GABAergic neurons, neuronal activation
- MeSH terms
- Animals, Disease Models, Animal, Epilepsy, Temporal Lobe, gamma-Aminobutyric Acid, Hippocampus, Immunohistochemistry, Interneurons, Male, Mice, Inbred C57BL, Neurons, Parvalbumins, Pilocarpine, Recurrence, Seizures, Temporal Lobe, Time Factors, Proto-Oncogene Proteins c-fos, Dentate Gyrus, Mice
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