Three-Dimensional Organization of Smooth Endoplasmic Reticulum in Hippocampal CA1 Dendrites and Dendritic Spines of the Immature and Mature Rat
Open accessAbstract
Recent studies have shown high levels of calcium in activated dendritic spines, where the smooth endoplasmic reticulum (SER) is likely to be important for regulating calcium. Here, the dimensions and organization of the SER in hippocampal spines and dendrites were measured through serial electron microscopy and three-dimensional analysis. SER of some form was found in 58% of the immature spines and in 48% of the adult spines. Less than 50% of the small spines at either age contained SER, suggesting that other mechanisms, such as cytoplasmic buffers, regulate ion fluxes within their small volumes. In contrast, >80% of the large mushroom spines of the adult had a spine apparatus, an organelle containing stacks of SER and dense-staining plates. Reconstructed SER occupied 0.001–0.022 μm 3, which was only 2–3.5% of the total spine volume; however, the convoluted SER membranes had surface areas of 0.12–2.19 μm 2, which were 12 to 40% of the spine surface area. Coated vesicles and multivesicular bodies occurred in some spines, suggesting local endocytotic activity. Smooth vesicles and tubules of SER were found in continuity with the spine plasma membrane and margins of the postsynaptic density (PSD), respectively, suggesting a role for the SER in the addition and recycling of spine membranes and synapses. The amount of SER in the parent dendrites was proportional to the number of spines and synapses originating along their lengths. These measurements support the hypothesis that the SER regulates the ionic and structural milieu of some, but not all, hippocampal dendritic spines.
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The spine twitching may involve the spine apparatus and translation machinery in the spine necks for protein synthesis-dependent spine-head enlargement.
“The spine twitching may involve the spine apparatus (9) and translation machinery in the spine necks (10, 11) for protein synthesis-dependent spine-head enlargement.”
From Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines (2008) · cites this paper as [9]
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- Authors
- Spacek, J., & Harris, K. M.
- Affiliations
- Boston Children's Hospital (US)
- ORCID
- Harris, Kristen M.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1997-01-01
- Volume
- 17
- Issue
- 1
- Pages
- 190-203
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6793680
- OpenAlex
- W2109950649
- Cited by
- 599
- References
- 2
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 16 · 2023: 13 · 2022: 24 · 2021: 29 · 2020: 22 · 2019: 29 · 2018: 19 · 2017: 19 · 2016: 26 · 2015: 26 · 2014: 25 · 2013: 20 · 2012: 11
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r03tw000178-01, NS21184, NS33574)
- Fogarty International Center (TW00178)
- National Institute of Neurological Disorders and Stroke (R01NS033574, NS21184, NS33574)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P30HD018655)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- smooth endoplasmic reticulum, dendritic spines, hippocampal CA1, dendrites, calcium regulation, spine apparatus, postsynaptic density, synaptic development, coated vesicles, multivesicular bodies, three-dimensional ultrastructure
- MeSH terms
- Aging, Animals, Dendrites, Endocytosis, Exocytosis, Hippocampus, Humans, Image Processing, Computer-Assisted, Male, Rats, Inbred Strains, Synapses, Endoplasmic Reticulum, Smooth, Rats
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