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Corticotropin-Releasing Factor Increases In Vitro Firing Rates of Serotonergic Neurons in the Rat Dorsal Raphe Nucleus: Evidence for Activation of a Topographically Organized Mesolimbocortical Serotonergic System
Open accessAbstract
In vivo studies suggest that the stress-related neuropeptide corticotropin-releasing factor (CRF) modulates serotonergic neurotransmission. To investigate the underlying mechanisms for this interaction, the present study examined the effects of CRF in vitro on dorsal raphe neurons that displayed electrophysiological and pharmacological properties consistent with a serotonergic phenotype. In the presence of either 1 or 2 mm Ca(2+), perfusion of ovine CRF or rat/human CRF rapidly and reversibly increased firing rates of a subpopulation (19 of 70, 27%) of serotonergic neurons predominantly located in the ventral portion of the dorsal raphe nucleus. For a given responsive neuron, the excitatory effects of CRF were reproducible, and there was no tachyphylaxis. Excitatory effects were dose-dependent (over the range of 0.1-1.6 micrometer) and were completely absent after exposure to the competitive CRF receptor antagonists alpha-helical CRF(9-41) or rat/human [d-Phe(12), Nle(21, 38), alpha-Me-Leu(37)]-CRF(12-41). Both the proportion of responsive neurons and the magnitude of excitatory responses to CRF in the ventral portion of the caudal dorsal raphe nucleus were markedly potentiated in slices prepared from animals previously exposed to isolation and daily restraint stress for 5 d. Immunohistochemical staining of the recorded slices revealed close associations between CRF-immunoreactive varicose axons and tryptophan hydroxylase-immunoreactive neurons in the area of the recordings, providing anatomical evidence for potential direct actions of CRF on serotonergic neurons. The electrophysiological properties and the distribution of responsive neurons within the dorsal raphe nucleus are consistent with the hypothesis that endogenous CRF activates a topographically organized mesolimbocortical serotonergic system.
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“Although DRN 5-HT neurons share a common developmental lineage (Deneris and Gaspar, 2018; Jensen et al., 2008), DRN 5-HT neurons in different subregions express different G protein-coupled receptors and vesicular transporters, suggesting spatial clustering of functionally specialized neurons (Calizo et al., 2011; Fernandez et al., 2016; Lowry et al., 2000; Templin et al., 2012).”
From Molecular and anatomical organization of the dorsal raphe nucleus (2019) · cites this paper as Lowry et al., 2000
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“Even bona fide DRN 5-HT neurons are molecularly, anatomically, and physiologically heterogeneous.”
From Molecular and anatomical organization of the dorsal raphe nucleus (2019) · cites this paper as Lowry et al., 2000
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Cites
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585
AChE-stained horizontal sections of the rat brain in stereotaxic coordinates
Paxinos, G., WATSON, C. R. R., & Emson, P. C. (1980) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(80)90021-7 · Cited by 4,514
Gray, J. A. (1982) · Behavioral and Brain Sciences · doi:10.1017/s0140525x00013066 · Cited by 4,477
Structure and function of the brain serotonin system
Jacobs, B. L., & Azmitia, E. C. (1992) · Physiological Reviews · doi:10.1152/physrev.1992.72.1.165 · Cited by 2,574
Vaughan, J. M., Donaldson, C. J., Bittencourt, J. C., Perrin, M. H., Lewis, K., Sutton, S. W., Chan, R. K. W., Turnbull, A. V., Lovejoy, D. A., RIVIER, C. L., et al. (1995) · Nature · doi:10.1038/378287a0 · Cited by 1,479
Physiology and pharmacology of corticotropin-releasing factor.
Owens, M. J., & Nemeroff, C. B. (1991) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06673-6 · Cited by 1,147
Pattern and time course of immediate early gene expression in rat brain following acute stress
Cullinan, W. E., Herman, J. P., Battaglia, D. F., Akil, H., & Watson, S. J. (1995) · Neuroscience · doi:10.1016/0306-4522(94)00355-9 · Cited by 1,056
Role of 5-HT in stress, anxiety, and depression
Graeff, F. G., Guimarães, F. S., De Andrade, T. G. C. S., & Deakin, J. F. W. (1996) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(95)02135-3 · Cited by 984
Chalmers, D., Lovenberg, T. W., & Souza, E. D. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-10-06340.1995 · Cited by 925
A PHA-L analysis of ascending projections of the dorsal raphe nucleus in the rat
Vertes, R. P. (1991) · Journal of Comparative Neurology · doi:10.1002/cne.903130409 · Cited by 842 · cited 2×
“…may be attributable to sampling of more rostral dorsal raphe serotonergic neurons (27.5 mm Bregma; Kirby et al., 2000) that are known to project preferentially to neocortical areas (Vertes, 1991), as well as to the substantia nigra and caudate putamen (Steinbusch et al., 1981; Imai et al., 1986).”
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Research works printed but not linked: 1
- Lowry, 1999, Corticotropin-releasing factor (CRF) increases in vitro firing rates of serotonergic neurones in the rat dorsal raphe nucleus: evidence for selective activation of a topographically organised mesolimbocortical serotonergic system., Soc Neurosci Abstr, 25, 75
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Stress, P. (2004)
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Furay, A. R., & Neumaier, J. F. (2013) · Neuromethods · doi:10.1007/978-1-62703-610-8_9
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Tuckwell, H. C., Zhou, Y., & Penington, N. J. (2017) · arXiv (Cornell University) · doi:10.48550/arxiv.1704.03941
Ángel, L.-V. M., Gutiérrez-Guzmán, B. E., Cervantes, M., & Olvera‐Cortés, M. E. (2010) · Humana Press eBooks · doi:10.1007/978-1-60761-941-3_24
Serotonergic involvement in the behavioral depression produced by intermittent swim stress
Christianson, J. P. (2006) · University of New Hampshire Scholars Repository (University of New Hampshire at Manchester)
Corticotropin-Releasing Factor in Brain function
Chen, P. J., & Yan, Z. (2006) · Springer eBooks · doi:10.1007/0-387-30395-2_26
Maximino, C. (2012) · SpringerBriefs in neuroscience · doi:10.1007/978-1-4614-4048-2_1
Fakheri, S. T., Changizi, R., Manouchehri, H., Emran, A. T. N., Ahangarkolaei, M. N., Saeedi, M., & Siahposht-Khachaki, A. (2026) · Journal of Applied Toxicology · doi:10.1002/jat.70092
Cellular and molecular coding of habit-driven behavior in the dorsal raphe nucleusPreprint
Chalençon, L., Rahn, R., Chottekalapanda, R. U., Lenz, A., Zhang, T., Donatelle, A., Stujenske, J. M., Fetcho, R. N., Parekh, P. K., Roshgadol, J., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.10.01.755903
Huang, K. W. (2019) · Harvard Dataverse · doi:10.7910/dvn/qb5cc8
Martinez‐Finley, E. J. (2010) · UNM’s Digital Repository (University of New Mexico)
Metadata
- Authors
- Lowry, C. A., Rodda, J. E., Lightman, S. L., & Ingram, C. D.
- Affiliations
- University of Bristol (GB)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2000-10-15
- Volume
- 20
- Issue
- 20
- Pages
- 7728-7736
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6772886
- OpenAlex
- W2109594449
- Cited by
- 211
- References
- 113
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 7 · 2014: 8 · 2013: 5 · 2012: 8
- FWCI
- 5.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Funders
- Wellcome Trust (045843/Z/95/Z)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- corticotropin-releasing factor, dorsal raphe nucleus, serotonergic neurons, neuronal firing rate, CRF receptor antagonists, tryptophan hydroxylase, stress, isolation stress, restraint stress, topographic organization
- MeSH terms
- Action Potentials, Animals, Axons, Calcium, Corticotropin-Releasing Hormone, Dose-Response Relationship, Drug, Immunohistochemistry, Male, Microelectrodes, Synaptic Transmission, Neurons, Raphe Nuclei, Reaction Time, Restraint, Physical, Serotonin, Stress, Physiological, Tryptophan Hydroxylase, Rats, Wistar, Serotonin Receptor Agonists, Rats, In Vitro Techniques
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