The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Effects of Progesterone Synthesized De Novo in the Developing Purkinje Cell on Its Dendritic Growth and Synaptogenesis
Open accessAbstract
De novo steroidogenesis from cholesterol is a conserved property of vertebrate brains, and such steroids synthesized de novo in the brain are called neurosteroids. The identification of neurosteroidogenic cells is essential to the understanding of the physiological role of neurosteroids in the brain. We have demonstrated recently that neuronal neurosteroidogenesis occurs in the brain and indicated that the Purkinje cell actively synthesizes several neurosteroids de novo from cholesterol in vertebrates. Interestingly, in the rat, this neuron actively synthesizes progesterone de novo from cholesterol only during neonatal life, when cerebellar cortical formation occurs most markedly. Therefore, in this study, the possible organizing actions of progesterone during cerebellar development have been examined. In vitro studies using cerebellar slice cultures from newborn rats showed that progesterone promotes dose-dependent dendritic outgrowth of Purkinje cells but dose not affect their somata. This effect was blocked by the anti-progestin RU 486 [mifepristone; 17beta-hydroxy-11beta-(4-methylaminophenyl)-17alpha-(1-propynyl) estra-4,9-dien-3 one-6-7]. In vivo administration of progesterone to pups further revealed an increase in the density of Purkinje spine synapses electron microscopically. In contrast to progesterone, there was no significant effect of 3alpha,5alpha-tetrahydroprogesterone, a progesterone metabolite, on Purkinje cell development. Reverse transcription-PCR-Southern and immunocytochemical analyses showed that intranuclear progesterone receptors were expressed in Purkinje cells. These results suggest that progesterone promotes both dendritic outgrowth and synaptogenesis in Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. Such organizing actions may contribute to the formation of the cerebellar neuronal circuit.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A simple method for organotypic cultures of nervous tissue
Stoppini, L., Buchs, P., & Muller, D. (1991) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(91)90128-m · Cited by 3,096
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.
Bottenstein, J. E., & SATO, G. H. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.514 · Cited by 2,268
Macdonald, R. L., & Olsen, R. W. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.003033 · Cited by 1,975
Gonadal steroids regulate dendritic spine density in hippocampal pyramidal cells in adulthood
Gould, E., Woolley, C., Frankfurt, M., & McEwen, B. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-04-01286.1990 · Cited by 1,406
Gonadal Steroid Induction of Structural Sex Differences in the Central Nervous System
Arnold, A. P., & Gorski, R. A. (1984) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.07.030184.002213 · Cited by 1,198
Naturally occurring fluctuation in dendritic spine density on adult hippocampal pyramidal neurons
Woolley, C., Gould, E., Frankfurt, M., & McEwen, B. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-12-04035.1990 · Cited by 1,107
Woolley, C. S., & McEwen, B. S. (1993) · The Journal of Comparative Neurology · doi:10.1002/cne.903360210 · Cited by 1,073
The nucleotide sequence of the rat cytoplasmic β–actin gene
Nudel, U., Zakut, R., Shani, M., Neuman, S., Levy, Z., & Yaffe, D. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.6.1759 · Cited by 1,025
Woolley, C. S., & McEwen, B. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-07-02549.1992 · Cited by 998
Research works printed but not linked: 2
- [19]19. Fuxe K, Gustafsson JA, Wetterberg L (1981) Steroid hormone regula- tion of the brain. Oxford: Pergamon.
- [51]51. Tsutsui K, Ukena K (2000) Neuronal neurosteroidogenesis in the cere- bellum. In: Proceedings of international symposium on molecular ste- roidogenesis (Okamoto M, Ishimura Y, Nawata H, eds), pp 397-400. Tokyo: Universal Academy.
Cited by
Sorted by citations
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458
Cholesterol homeostasis and function in neurons of the central nervous system
Pfrieger, F. W. (2003) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-003-3018-7 · Cited by 453
do Rego, J. L., Seong, J. Y., Burel, D., Leprince, J., Luu-The, V., Tsutsui, K., Tonon, M., Pelletier, G., & Vaudry, H. (2009) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2009.05.006 · Cited by 365
Self-citationSchumacher, M., Mattern, C., Ghoumari, A. M., Oudinet, J., Lière, P., Labombarda, F., Sitruk-Ware, R. L., Nicola, A. F. D., & Guennoun, R. (2013) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2013.09.004 · Cited by 368
Ghoumari, A. M., Ibanez, C., El‐Etr, M., Leclerc, P., Eychenne, B., O’Malley, B. W., Baulieu, E. E., & Schumacher, M. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01881.x · Cited by 291
Metadata
- Authors
- Sakamoto, H., Ukena, K., & Tsutsui, K.
- Affiliations
- Hiroshima University (JP)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2001-08-15
- Volume
- 21
- Issue
- 16
- Pages
- 6221-6232
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6763166
- OpenAlex
- W1783731643
- Semantic Scholar
- 2b9eec77bf495ccf9efdb769ce7bc68a15546ed9
- Cited by
- 193
- References
- 64
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 9 · 2018: 3 · 2017: 5 · 2016: 9 · 2015: 6 · 2014: 7 · 2013: 6 · 2012: 16
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- PubMed
- Funders
- University of Central Lancashire
- Japan Society for the Promotion of Science (11354010, 12440233)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Keywords
- progesterone, neurosteroidogenesis, Purkinje cells, dendritic outgrowth, synaptogenesis, cerebellar development, RU486, cerebellar slice cultures, neural circuit formation
- MeSH terms
- Animals, Animals, Newborn, Cerebellum, Dendrites, Dose-Response Relationship, Drug, Female, Hormone Antagonists, Male, Pregnanolone, Progesterone, Purkinje Cells, Rats, Inbred F344, Receptors, Progesterone, RNA, Messenger, Synapses, Mifepristone, Receptors, Cytoplasmic and Nuclear, Rats, Calbindins, S100 Calcium Binding Protein G, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
