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Effects of Progesterone Synthesized De Novo in the Developing Purkinje Cell on Its Dendritic Growth and Synaptogenesis
Open accessAbstract
De novo steroidogenesis from cholesterol is a conserved property of vertebrate brains, and such steroids synthesized de novo in the brain are called neurosteroids. The identification of neurosteroidogenic cells is essential to the understanding of the physiological role of neurosteroids in the brain. We have demonstrated recently that neuronal neurosteroidogenesis occurs in the brain and indicated that the Purkinje cell actively synthesizes several neurosteroids de novo from cholesterol in vertebrates. Interestingly, in the rat, this neuron actively synthesizes progesterone de novo from cholesterol only during neonatal life, when cerebellar cortical formation occurs most markedly. Therefore, in this study, the possible organizing actions of progesterone during cerebellar development have been examined. In vitro studies using cerebellar slice cultures from newborn rats showed that progesterone promotes dose-dependent dendritic outgrowth of Purkinje cells but dose not affect their somata. This effect was blocked by the anti-progestin RU 486 [mifepristone; 17beta-hydroxy-11beta-(4-methylaminophenyl)-17alpha-(1-propynyl) estra-4,9-dien-3 one-6-7]. In vivo administration of progesterone to pups further revealed an increase in the density of Purkinje spine synapses electron microscopically. In contrast to progesterone, there was no significant effect of 3alpha,5alpha-tetrahydroprogesterone, a progesterone metabolite, on Purkinje cell development. Reverse transcription-PCR-Southern and immunocytochemical analyses showed that intranuclear progesterone receptors were expressed in Purkinje cells. These results suggest that progesterone promotes both dendritic outgrowth and synaptogenesis in Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. Such organizing actions may contribute to the formation of the cerebellar neuronal circuit.
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Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A simple method for organotypic cultures of nervous tissue
Stoppini, L., Buchs, P., & Muller, D. (1991) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(91)90128-m · Cited by 3,096
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.
Bottenstein, J. E., & SATO, G. H. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.514 · Cited by 2,268
Macdonald, R. L., & Olsen, R. W. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.003033 · Cited by 1,975
Gonadal steroids regulate dendritic spine density in hippocampal pyramidal cells in adulthood
Gould, E., Woolley, C., Frankfurt, M., & McEwen, B. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-04-01286.1990 · Cited by 1,406
Gonadal Steroid Induction of Structural Sex Differences in the Central Nervous System
Arnold, A. P., & Gorski, R. A. (1984) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.07.030184.002213 · Cited by 1,198
Naturally occurring fluctuation in dendritic spine density on adult hippocampal pyramidal neurons
Woolley, C., Gould, E., Frankfurt, M., & McEwen, B. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-12-04035.1990 · Cited by 1,107
Woolley, C. S., & McEwen, B. S. (1993) · The Journal of Comparative Neurology · doi:10.1002/cne.903360210 · Cited by 1,073
The nucleotide sequence of the rat cytoplasmic β–actin gene
Nudel, U., Zakut, R., Shani, M., Neuman, S., Levy, Z., & Yaffe, D. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.6.1759 · Cited by 1,025
Woolley, C. S., & McEwen, B. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-07-02549.1992 · Cited by 998
Altman, J. (1972) · Journal of Comparative Neurology · doi:10.1002/cne.901450402 · Cited by 996
Altman, J. (1972) · The Journal of Comparative Neurology · doi:10.1002/cne.901450305 · Cited by 912
Characterization and measurement of dehydroepiandrosterone sulfate in rat brain
Corpéchot, C., Robel, P., Axelson, M., Sjövall, J., & Baulieu, E. E. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.8.4704 · Cited by 656
(1997) · PubMed · Cited by 643
Progesterone synthesis and myelin formation by Schwann cells
Koenig, H. L., Schumacher, M., Ferzaz, B., Thi, A. N. D., Ressouches, A., Guennoun, R., Jung-Testas, I., Robel, P., Akwa, Y., & Baulieu, E.-E. (1995) · Science · doi:10.1126/science.7770777 · Cited by 480
Estrogen-Mediated Structural and Functional Synaptic Plasticity in the Female Rat Hippocampus
Woolley, C. S. (1998) · Hormones and Behavior · doi:10.1006/hbeh.1998.1466 · Cited by 398
Pregnenolone and its sulfate ester in the rat brain
Corpéchot, C., Synguelakis, M., Talha, S., Axelson, M., Sjövall, J., Vihko, R., Baulieu, E.-E., & Robel, P. (1983) · Brain Research · doi:10.1016/0006-8993(83)90797-7 · Cited by 385 · cited 5×
“On the other hand, dehydroepiandrosterone (DHEA) and its sulfate ester (DHEAS) are also abundant neurosteroids in the mamma- lian brain (Corpéchot et al., 1981, 1983; Jo et al., 1989).”
BackgroundMethodInfluentialNeurosteroids: Cytochrome P-450 scc in Rat Brain
Goascogne, C. L., Röbel, P., Gouézou, M., Sananès, N., Baulieu, É.-É., & Waterman, M. R. (1987) · Science · doi:10.1126/science.3306919 · Cited by 363
Park-Sarge, O.-K., & Mayo, K. E. (1991) · Molecular Endocrinology · doi:10.1210/mend-5-7-967 · Cited by 338
Compagnone, N. A., & Mellon, S. H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4678 · Cited by 327
Baulieu, É.-É., & Röbel, P. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4089 · Cited by 318
Neurosteroids: a new function in the brain
Baulieu, É.-É. (1991) · Biology of the Cell · doi:10.1016/0248-4900(91)90045-o · Cited by 308
Neurosteroids: oligodendrocyte mitochondria convert cholesterol to pregnenolone.
Hu, Z. Y., Bourreau, E., Jung‐Testas, I., Röbel, P., & Baulieu, E. E. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.23.8215 · Cited by 293
Electrical activity in cerebellar cultures determines Purkinje cell dendritic growth patterns
Schilling, K., Dickinson, M. H., Connor, J. A., & Morgan, J. I. (1991) · Neuron · doi:10.1016/0896-6273(91)90335-w · Cited by 253
Novel brain function: biosynthesis and actions of neurosteroids in neurons
Tsutsui, K., Ukena, K., Usui, M., Sakamoto, H., & Takase, M. (2000) · Neuroscience Research · doi:10.1016/s0168-0102(99)00132-7 · Cited by 235
Self-citationCruz-Orive, L. M. (1983) · Journal of Microscopy · doi:10.1111/j.1365-2818.1983.tb04255.x · Cited by 197
Effects of estrogen in the CNS
Woolley, C. S. (1999) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(99)80051-8 · Cited by 192
Ukena, K., Usui, M., Kohchi, C., & Tsutsui, K. (1998) · Endocrinology · doi:10.1210/endo.139.1.5672 · Cited by 177
Self-citationGreco, T. L., & Payne, A. H. (1994) · Endocrinology · doi:10.1210/endo.135.1.8013361 · Cited by 170
Haywood, S. A., Simonian, S. X., van der Beek, E. M., Bicknell, R. J., & Herbison, A. E. (1999) · Endocrinology · doi:10.1210/endo.140.7.6869 · Cited by 167
Akwa, Y., Young, J., Kabbadj, K., Sancho, M. J., Zucman, D., Vourc’h, C., Jung‐Testas, I., Hu, Z. Y., Goascogne, C. L., Jo, D. H., et al. (1991) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(91)90169-6 · Cited by 166
Shimada, A., Mason, C. A., & Morrison, M. E. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-21-08559.1998 · Cited by 165
Avian neurosteroids. I. Pregnenolone biosynthesis in the quail brain
Tsutsui, K., & Yamazaki, T. (1995) · Brain Research · doi:10.1016/0006-8993(95)00116-8 · Cited by 152
Self-citationGene Expression of Progesterone Receptor Isoforms in the Rat Brain
Kato, J., Hirata, S., Nozawa, A., & Yamada-Mouri, N. (1994) · Hormones and Behavior · doi:10.1006/hbeh.1994.1043 · Cited by 149
Ukena, K., Kohchi, C., & Tsutsui, K. (1999) · Endocrinology · doi:10.1210/endo.140.2.6516 · Cited by 141
Self-citationPark-Sarge, O. K., & Mayo, K. E. (1994) · Endocrinology · doi:10.1210/endo.134.2.8299566 · Cited by 139
Control of spine formation by electrical activity in the adult rat cerebellum
Bravin, M., Morando, L., Vercelli, A., Rossi, F., & Strata, P. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.4.1704 · Cited by 122
Pregnenolone, dehydroepiandrosterone, and their sulfate and fatty acid esters in the rat brain
Jo, D.-H., Abdallah, M. A., Young, J., Baulieu, É.-É., & Röbel, P. (1989) · Steroids · doi:10.1016/0039-128x(89)90003-2 · Cited by 120
Neurosteroid biosynthesis in vertebrate brains
Tsutsui, K., Ukena, K., Takase, M., Kohchi, C., & Lea, R. W. (1999) · Comparative Biochemistry and Physiology Part C Pharmacology Toxicology and Endocrinology · doi:10.1016/s0742-8413(99)00065-1 · Cited by 112
Self-citationPotential Role of Maternal Progesterone in the Sexual Differentiation of the Brain
Wagner, C. K., Nakayama, A. Y., & De Vries, G. J. (1998) · Endocrinology · doi:10.1210/endo.139.8.6223 · Cited by 111
Takase, M., Ukena, K., Yamazaki, T., Kominami, S., & Tsutsui, K. (1999) · Endocrinology · doi:10.1210/endo.140.4.6641 · Cited by 111
Self-citationBrinton, R. D. (1994) · Journal of Neuroscience · doi:10.1523/jneurosci.14-05-02763.1994 · Cited by 108
Avian neurosteroids. II. Localization of a cytochrome P450scc-like substance in the quail brain
Usui, M., Yamazaki, T., Kominami, S., & Tsutsui, K. (1995) · Brain Research · doi:10.1016/0006-8993(95)00117-9 · Cited by 106
Self-citationProgesterone Synthesized by Schwann Cells during Myelin Formation Regulates Neuronal Gene Expression
Chan, J. R., Rodriguez‐Waitkus, P., Ng, B. K., Liang, P., & Gläser, M. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.7.2283 · Cited by 99
Progesterone receptor isoforms expression pattern in the rat brain during the estrotts cycle
Guerra‐Araiza, C., Cerbón, M. A., Morimoto, S., & Camacho‐Arroyo, I. (2000) · Life Sciences · doi:10.1016/s0024-3205(00)00497-5 · Cited by 96
Progesterone receptor isoforms are differentially regulated by sex steroids in the rat forebrain
Camacho‐Arroyo, I., Guerra‐Araiza, C., & Cerbón, M. A. (1998) · Neuroreport · doi:10.1097/00001756-199812210-00001 · Cited by 92
Ukena, K., Honda, Y., Inai, Y., Kohchi, C., Lea, R. W., & Tsutsui, K. (1999) · Brain Research · doi:10.1016/s0006-8993(98)01296-7 · Cited by 84
Self-citationTraish, A. M., & Wotiz, H. H. (1990) · Endocrinology · doi:10.1210/endo-127-3-1167 · Cited by 77
Szabó, M., Kilen, S. M., Nho, S. J., & Schwartz, N. B. (2000) · Biology of Reproduction · doi:10.1095/biolreprod62.1.95 · Cited by 74
Jung‐Testas, I., Thi, A. D., Koenig, H. L., Désarnaud, F., Shazand, K., Schumacher, M., & Baulieu, E. E. (1999) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(98)00149-6 · Cited by 73
The ontogeny of gene expression of progestin receptors in the female rat brain
Kato, J., Hirata, S., Nozawa, A., & Mouri, N. (1993) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(93)90072-5 · Cited by 68
Neurosteroids in the cerebellar Purkinje neuron and their actions (review).
Tsutsui, K., & Ukena, K. (1999) · International Journal of Molecular Medicine · doi:10.3892/ijmm.4.1.49 · Cited by 56
Self-citationTanaka, M., Tomita, A., Yoshida, S., Yano, M., & Shimizu, H. (1994) · Brain Research · doi:10.1016/0006-8993(94)90161-9 · Cited by 55
Progesterone receptor expression in the brainstem of the female rat
Kastrup, Y., Hallbeck, M., Amandusson, Å., Hirata, S., Hermanson, O., & Blomqvist, A. G. (1999) · Neuroscience Letters · doi:10.1016/s0304-3940(99)00753-3 · Cited by 53
Gruol, D. L., & Crimi, C. P. (1988) · Developmental Brain Research · doi:10.1016/0165-3806(88)90177-0 · Cited by 47
Tauer, U., Volk, B., & Heimrich, B. (1996) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.1996.tb01115.x · Cited by 44
Messer, A. C., Mazurkiewicz, J. E., & Maskin, P. (1981) · Cellular and Molecular Neurobiology · doi:10.1007/bf00736042 · Cited by 36
Sakamoto, H., Ubuka, T., Kohchi, C., Li, D., Ukena, K., & Tsutsui, K. (2000) · Endocrinology · doi:10.1210/endo.141.12.7827 · Cited by 30
Self-citationTsutsui, K. (1991) · Endocrinology · doi:10.1210/endo-128-1-477 · Cited by 29
Self-citationPhenytoin alters Purkinje cell axon morphology and targeting in vitro
Tauer, U., Knoth, R., & Volk, B. (1998) · Acta Neuropathologica · doi:10.1007/s004010050844 · Cited by 23
Fuxé, K., Gustafsson, J.-Α., Wetterberg, L., & samfundet, W.-G. (1981) · Cited by 18
Neurosteroids in the avian brain
Tsutsui, K. (1997) · Medical Entomology and Zoology · Cited by 18
Self-citationTsutsui, K., Li, D., Ukena, K., Kikuchi, M., & Ishii, S. (1998) · Endocrinology · doi:10.1210/endo.139.10.6224 · Cited by 14
Self-citation
Research works printed but not linked: 2
- [19]19. Fuxe K, Gustafsson JA, Wetterberg L (1981) Steroid hormone regula- tion of the brain. Oxford: Pergamon.
- [51]51. Tsutsui K, Ukena K (2000) Neuronal neurosteroidogenesis in the cere- bellum. In: Proceedings of international symposium on molecular ste- roidogenesis (Okamoto M, Ishimura Y, Nawata H, eds), pp 397-400. Tokyo: Universal Academy.
Cited by
Sorted by citations
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458
Cholesterol homeostasis and function in neurons of the central nervous system
Pfrieger, F. W. (2003) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-003-3018-7 · Cited by 453
do Rego, J. L., Seong, J. Y., Burel, D., Leprince, J., Luu-The, V., Tsutsui, K., Tonon, M., Pelletier, G., & Vaudry, H. (2009) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2009.05.006 · Cited by 365
Self-citationSchumacher, M., Mattern, C., Ghoumari, A. M., Oudinet, J., Lière, P., Labombarda, F., Sitruk-Ware, R. L., Nicola, A. F. D., & Guennoun, R. (2013) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2013.09.004 · Cited by 368
Ghoumari, A. M., Ibanez, C., El‐Etr, M., Leclerc, P., Eychenne, B., O’Malley, B. W., Baulieu, E. E., & Schumacher, M. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01881.x · Cited by 291
Metadata
- Authors
- Sakamoto, H., Ukena, K., & Tsutsui, K.
- Affiliations
- Hiroshima University (JP)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2001-08-15
- Volume
- 21
- Issue
- 16
- Pages
- 6221-6232
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6763166
- OpenAlex
- W1783731643
- Semantic Scholar
- 2b9eec77bf495ccf9efdb769ce7bc68a15546ed9
- Cited by
- 193
- References
- 64
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 9 · 2018: 3 · 2017: 5 · 2016: 9 · 2015: 6 · 2014: 7 · 2013: 6 · 2012: 16
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- PubMed
- Funders
- University of Central Lancashire
- Japan Society for the Promotion of Science (11354010, 12440233)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Keywords
- progesterone, neurosteroidogenesis, Purkinje cells, dendritic outgrowth, synaptogenesis, cerebellar development, RU486, cerebellar slice cultures, neural circuit formation
- MeSH terms
- Animals, Animals, Newborn, Cerebellum, Dendrites, Dose-Response Relationship, Drug, Female, Hormone Antagonists, Male, Pregnanolone, Progesterone, Purkinje Cells, Rats, Inbred F344, Receptors, Progesterone, RNA, Messenger, Synapses, Mifepristone, Receptors, Cytoplasmic and Nuclear, Rats, Calbindins, S100 Calcium Binding Protein G, In Vitro Techniques
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