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The EGL-21 Carboxypeptidase E Facilitates Acetylcholine Release at Caenorhabditis elegans Neuromuscular Junctions
Open accessAbstract
Proneuropeptides are packaged into dense-core vesicles in which they are processed into active peptides by copackaged enzymes. Proprotein convertases (PCs) cleave precursors after dibasic residues, and carboxypeptidases remove basic residues from the C terminals. We show here that the Caenorhabditis elegans egl-21 gene encodes a protein that is very similar to carboxypeptidase E (CPE) and is broadly expressed in the nervous system. Mutants lacking either egl-21 CPE or egl-3, which encodes the C. elegans ortholog of PC type 2 (PC2), were defective for processing endogenously expressed FMRFamide (Phe-Met-Arg-Phe-NH2)-related peptides (FaRPs). Mutants lacking the unc-104 kinesin motor protein were defective for anterograde movement of dense-core vesicle components, including egl-3 PC2,egl-21 CPE, and FaRPs. We provide evidence that egl-3 PC2 and egl-21 CPE mutants have diminished acetylcholine release at neuromuscular junctions (NMJs). Taken together, these results suggest that egl-21 CPE and egl-3 PC2 process endogenous neuropeptides that facilitate acetylcholine release at C. elegans NMJs.
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Cited by
Cao, J., Packer, J. S., Ramani, V., Cusanovich, D. A., Huynh, C., Daza, R., Qiu, X., Lee, C., Furlan, S. N., Steemers, F. J., et al. (2017) · doi:10.1101/104844 · Cited by 1,402
Speese, S., Petrie, M., Schuske, K., Ailion, M., Ann, K., Iwasaki, K., Jorgensen, E. M., & Martin, T. F. J. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.1466-07.2007 · Cited by 333
The neuronal genome of Caenorhabditis elegans
Hobert, O. (2013) · WormBook · doi:10.1895/wormbook.1.161.1 · Cited by 279
Ramot, D., Johnson, B. E., Berry, T. L., Carnell, L., & Goodman, M. B. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002208 · Cited by 278
Charlie, N. K., Schade, M. A., Thomure, A. M., & Miller, K. G. (2006) · Genetics · doi:10.1534/genetics.105.049577 · Cited by 98
Metadata
- Authors
- Jacob, T. C., & Kaplan, J. M.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2003-03-15
- Volume
- 23
- Issue
- 6
- Pages
- 2122-2130
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W2143225227
- Cited by
- 163
- References
- 0
- FWCI
- 2.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jacob, T. C., & Kaplan, J. M. (2003). The EGL-21 Carboxypeptidase E Facilitates Acetylcholine Release at Caenorhabditis elegans Neuromuscular Junctions. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.23-06-02122.2003
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