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Small-Conductance Ca2+-Activated K+Channel Type 2 (SK2) Modulates Hippocampal Learning, Memory, and Synaptic Plasticity
Open accessAbstract
Apamin-sensitive, small-conductance, Ca 2+-activated K+channels (SK channels) modulate neuronal excitability in CA1 neurons. Blocking all SK channel subtypes with apamin facilitates the induction of hippocampal synaptic plasticity and enhances hippocampal learning. In CA1 dendrites, SK channels are activated by Ca 2+through NMDA receptors and restrict glutamate-mediated EPSPs. Studies of SK channel knock-out mice reveal that of the three apamin-sensitive SK channel subunits (SK1–SK3), only SK2 subunits are necessary for the apamin-sensitive currents in CA1 hippocampal neurons. To determine the specific influence of SK2 channels on hippocampal synaptic plasticity, learning, and memory, we used gene targeting through homologous recombination in embryonic stem cells to generate transgenic mice that overexpress SK2 subunits by 10-fold (SK2+/T). In these mice, the apamin-sensitive current in CA1 neurons was increased by approximately fourfold, relative to wild-type (WT) littermates. In addition, the amplitude of synaptically evoked EPSPs recorded from SK2+/T CA1 neurons increased twice as much in response to SK channel blockade relative to EPSPs recorded from WT CA1 neurons. Consistent with this, SK2 overexpression reduced long-term potentiation after high-frequency stimulation compared with WT littermates and severely impaired learning in both hippocampus- and amygdala-dependent tasks. We conclude that SK2 channels regulate hippocampal synaptic plasticity and play a critical role in modulating mechanisms of learning and memory.
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Metadata
- Authors
- Hammond, R. S., Bond, C. T., Strassmaier, T., Jennifer Ngo-Anh, T., Adelman, J. P., Maylie, J., & Stackman, R. W.
- Affiliations
- Oregon Health & Science University (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2006-02-08
- Volume
- 26
- Issue
- 6
- Pages
- 1844-1853
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6793641
- OpenAlex
- W2109279225
- Cited by
- 215
- References
- 39
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 6 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 11 · 2019: 10 · 2018: 5 · 2017: 7 · 2016: 8 · 2015: 10 · 2014: 8 · 2013: 12 · 2012: 20
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- small conductance calcium-activated potassium channels, SK2 channel, apamin, hippocampus, CA1 neurons, synaptic plasticity, long-term potentiation, learning and memory, overexpression, NMDA receptor
- MeSH terms
- Animals, Electrophysiology, Heterozygote, Hippocampus, Learning, Memory, Mice, Inbred C57BL, Neuronal Plasticity, Synapses, Polymerase Chain Reaction, DNA Primers, Excitatory Postsynaptic Potentials, Mice, Small-Conductance Calcium-Activated Potassium Channels, In Vitro Techniques
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