Control of the postsynaptic membrane viscosity
Open accessAbstract
The physical properties of the postsynaptic membrane (PSM), including its viscosity, determine its capacity to regulate the net flux of synaptic membrane proteins such as neurotransmitter receptors. To address these properties, we studied the lateral diffusion of glycophosphatidylinositol-anchored green fluorescent protein and cholera toxin bound to the external leaflet of the plasma membrane. Relative to extrasynaptic regions, their mobility was reduced at synapses and even more at inhibitory than at excitatory ones. This indicates a higher density of obstacles and/or higher membrane viscosity at inhibitory contacts. Actin depolymerization reduced the confinement and accelerated a population of fast, mobile molecules. The compaction of obstacles thus depends on actin cytoskeleton integrity. Cholesterol depletion increased the mobility of the slow diffusing molecules, allowing them to diffuse more rapidly through the crowded PSM. Thus, the PSM has lipid-raft properties, and the density of obstacles to diffusion depends on filamentous actin. Therefore, lipid composition and actin-dependent protein compaction regulate viscosity of the PSM and, consequently, the molecular flow in and out of synapses.
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Crowding in the spine head may contribute to diffusional trapping of receptors.
“Based on results from modeling (Renner et al., 2009) it has been suggested that crowding in the spine head may contribute to this phenomenon (Colgan and Yasuda, 2014).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Renner et al., 2009
The Discussion says that obstacles at postsynaptic membranes increase apparent viscosity and favor receptor trapping. In “Diffusion of QD-ChTx depends on cytoskeleton integrity,” the authors describe the postsynaptic membrane as probably crowded and say crowding restricts diffusion. The copy does not establish crowding throughout the spine head or present this as a result of modeling; the reported measurements primarily track lipid-bound probes. (LLM, conf=0.85)
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Cites
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AMPARs and Synaptic Plasticity: The Last 25 Years
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Deleterious Effects of Amyloid β Oligomers Acting as an Extracellular Scaffold for mGluR5
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“Based on results from modeling (Renner et al., 2009) it has been suggested that crowding in the spine head may contribute to this phenomenon (Colgan and Yasuda, 2014).”
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Self-citation
- Authors
- Renner, M., Choquet, D., & Triller, A.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Triller, Antoine
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2009-03-04
- Volume
- 29
- Issue
- 9
- Pages
- 2926-2937
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6666215
- OpenAlex
- W1971067804
- Cited by
- 145
- References
- 4
- Citations by year
- 2025: 4 · 2023: 1 · 2022: 7 · 2021: 10 · 2020: 9 · 2019: 8 · 2018: 2 · 2017: 8 · 2016: 10 · 2015: 13 · 2014: 15 · 2013: 8 · 2012: 9
- FWCI
- 6.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- Funders
- Agence Nationale de la Recherche
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- postsynaptic membrane, membrane viscosity, lateral diffusion, lipid rafts, actin cytoskeleton, cholesterol depletion, inhibitory synapses, excitatory synapses, neurotransmitter receptors, cholera toxin
- MeSH terms
- Actins, Animals, Cells, Cultured, Cholera Toxin, Cholesterol, Kinetics, Phosphatidylinositols, Synaptic Membranes, Transfection, Viscosity, Rats, Sprague-Dawley, Receptors, GABA, Receptors, AMPA, Membrane Microdomains, Green Fluorescent Proteins, Rats
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