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Functional Neural Development from Human Embryonic Stem Cells: Accelerated Synaptic Activity via Astrocyte Coculture
Open accessAbstract
How a naive human neuroepithelial cell becomes an electrophysiologically active neuron remains unknown. Here, we describe the early physiological development of neurons differentiating from naive human embryonic stem (hES) cells. We found that differentiating neuronal cells progressively decrease their resting membrane potential, gain characteristic Na+ and K+ currents, and fire mature action potentials by 7 weeks of differentiation. This is similar to the maturation pattern observed in animals, albeit on a greatly expanded time scale. An additional 3 weeks of differentiation resulted in neurons that could fire repetitive trains of action potentials in response to depolarizing current pulses. The onset of spontaneous synaptic activity also occurred after 7 weeks of differentiation, in association with the differentiation of astrocytes within the culture. Cocultures of hES cell-derived neuroepithelial cells with exogenous astrocytes significantly accelerated the onset of synaptic currents but did not alter action potential generation. These findings suggest that the development of membrane characteristics and action potentials depend on the intrinsic maturation of Na+ and K+ currents, whereas synaptic transmission is enhanced by astrocytes, which may be achieved independently of the maturation of action potentials. Furthermore, we found that although astrocyte-conditioned medium accelerated synaptic protein localization, it did not increase synaptic activity, suggesting a contact-dependent mechanism by which astrocytes augment synaptic activity. These results lay the foundation for future studies examining the functional development of human neurons and provide support for the potential application of human cells in restorative neuronal therapies.
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“neurons after ~4 weeks could be induced to fire multiple action potentials”
From Metabolic reprogramming during neuronal differentiation from aerobic glycolysis to neuronal oxidative phosphorylation (2016) · cites this paper as Johnson et al., 2007
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Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz‐Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,947
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
Reubinoff, B. E., Pera, M. F., Fong, C., Trounson, A. O., & Bongso, A. (2000) · Nature Biotechnology · doi:10.1038/74447 · Cited by 2,708
Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo
Hodgkin, A. L., & Huxley, A. F. (1952) · The Journal of Physiology · doi:10.1113/jphysiol.1952.sp004717 · Cited by 2,466
In vitro differentiation of transplantable neural precursors from human embryonic stem cells
Zhang, S., Wernig, M., Duncan, I. D., Brüstle, O., & Thomson, J. A. (2001) · Nature Biotechnology · doi:10.1038/nbt1201-1129 · Cited by 1,935
Self-citationCNS synaptogenesis promoted by glia-derived cholesterol
Mauch, D. H., Nägler, K., Schumacher, S., Göritz, C., Müller, E.-C., Otto, A., & Pfrieger, F. W. (2001) · Science · doi:10.1126/science.294.5545.1354 · Cited by 1,711
Thrombospondins are astrocyte-secreted proteins that promote CNS synaptogenesis
Christopherson, K. S., Ullian, E. M., Stokes, C. C. A., Mullowney, C. E., Hell, J. W., Agah, A., Lawler, J., Mosher, D. F., Bornstein, P., & Barres, B. A. (2005) · Cell · doi:10.1016/j.cell.2004.12.020 · Cited by 1,688
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
Control of Synapse Number by Glia
Ullian, E. M., Sapperstein, S. K., Christopherson, K. S., & Barres, B. A. (2001) · Science · doi:10.1126/science.291.5504.657 · Cited by 1,380
Translating developmental time across mammalian species
Clancy, B., Darlington, R. B., & Finlay, B. L. (2001) · Neuroscience · doi:10.1016/s0306-4522(01)00171-3 · Cited by 1,221
Embryonic Stem Cells Express Neuronal Properties in Vitro
Bain, G., Kitchens, D. L., Yao, M., Huettner, J. E., & Gottlieb, D. I. (1995) · Developmental Biology · doi:10.1006/dbio.1995.1085 · Cited by 1,168
Cited by
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Items 201–300 of 324
Prajerová, I., Honsa, P., Chvátal, A., & Anděrová, M. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2010.09.023 · Cited by 18
Izsák, J., Seth, H., Theiss, S., Hanse, E., & Illes, S. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.05.006 · Cited by 16
Importance of being Nernst: Synaptic activity and functional relevance in stem cell-derived neurons
Bradford, A. B. (2015) · World Journal of Stem Cells · doi:10.4252/wjsc.v7.i6.899 · Cited by 17
Urrutia-Cabrera, D., Liou, R. H.-C., Lin, J., Shi, Y. X., Liu, K., Hung, S. S. C., Hewitt, A. W., Wang, P., & Wong, R. C. B. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c17975 · Cited by 17
Stem cell‐derived astrocytes: are they physiologically credible?
Hill, E. J., Nagel, D. A., Parri, H. R., & Coleman, M. D. (2015) · The Journal of Physiology · doi:10.1113/jp270658 · Cited by 18
Interleukin-6-Mediated Induced Pluripotent Stem Cell (iPSC)-Derived Neural Differentiation
Sulistio, Y. A., Lee, H. K., Jung, S. J., & Heese, K. (2017) · Molecular Neurobiology · doi:10.1007/s12035-017-0594-3 · Cited by 17
Lee, J.-H., Liu, J.-W., Lin, S., Harn, H., & Chiou, T. (2018) · Cell Transplantation · doi:10.1177/0963689718785154 · Cited by 21
White, N., & Sakiyama‐Elbert, S. E. (2018) · Developmental Dynamics · doi:10.1002/dvdy.24680 · Cited by 17
Prajerová, I., Honsa, P., Chvátal, A., & Anděrová, M. (2009) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-009-9443-x · Cited by 16
The use of human neurons for novel drug discovery in dementia research
Vargas‐Caballero, M., Willaime‐Morawek, S., Gómez‐Nicola, D., Perry, V. H., Bulters, D. O., & Mudher, A. K. (2016) · Expert Opinion on Drug Discovery · doi:10.1517/17460441.2016.1154528 · Cited by 15
Pharmacological Profiling of Purified Human Stem Cell-Derived and Primary Mouse Motor Neurons
Moakley, D. F., Koh, J., Pereira, J. D., DuBreuil, D. M., Devlin, A., Berezovski, E., Zhu, K., & Wainger, B. J. (2019) · Scientific Reports · doi:10.1038/s41598-019-47203-7 · Cited by 16
Hsu, Y., Chen, S.-L., Wang, Y.-J., Chen, Y., Wang, D.-Y., Chen, L., Chen, C., Chen, H., & Chiu, I. (2014) · Stem Cells Translational Medicine · doi:10.5966/sctm.2013-0111 · Cited by 16
Harbom, L. J., Rudisill, T., Michel, N., Litwa, K., Beenhakker, M. P., & McConnell, M. J. (2018) · Cell and Tissue Research · doi:10.1007/s00441-018-2942-7 · Cited by 16
Morgan, P. J., Liedmann, A., Hübner, R., Hovakimyan, M., Rolfs, A., & Frech, M. J. (2011) · Stem Cells and Development · doi:10.1089/scd.2011.0335 · Cited by 16
Towards physiologically relevant human pluripotent stem cell (hPSC) models of Parkinson’s disease
Coccia, E., & Ahfeldt, T. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02326-5 · Cited by 16
Functional differentiation of a clone resembling embryonic cortical interneuron progenitors
Li, H., Han, Y. R., Bi, C., Davila, J., Goff, L. A., Thompson, K., Swerdel, M. R., Camarillo, C., Ricupero, C. L., Hart, R. P., et al. (2008) · Developmental Neurobiology · doi:10.1002/dneu.20679 · Cited by 15
Rajendrarao, S., Manjunatha, V. M., Sabitha, R., Alladi, P. A., Nalini, A., Rao, L. T., Sagar, B. K. C., Steinbusch, H. W. M., Kramer, B. W. W., Sathyaprabha, T. N., et al. (2018) · Molecular Neurobiology · doi:10.1007/s12035-018-1149-y · Cited by 15
Stem cells in development of therapeutics for Parkinson's disease: A perspective
Xi, J., & Zhang, S. (2008) · Journal of Cellular Biochemistry · doi:10.1002/jcb.21916 · Cited by 15
Self-citationEngineering circuits of human iPSC-derived neurons and rat primary glia
Girardin, S., Ihle, S. J., Menghini, A., Krubner, M., Tognola, L., Duru, J., Fruh, I., Fruh, I., Müller, M., & Vörös, J. (2023) · Frontiers in Neuroscience · doi:10.3389/fnins.2023.1103437 · Cited by 12
Perspectives for computational modeling of cell replacement for neurological disorders
Aimone, J. B., & Weick, J. P. (2013) · Frontiers in Computational Neuroscience · doi:10.3389/fncom.2013.00150 · Cited by 15
Self-citationHuman stem cells as a model of motoneuron development and diseases
Liu, Y., & Zhang, S. (2010) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2010.05537.x · Cited by 14
Self-citationPhysiological Properties of Human Fetal Cortex In Vitro
Moore, A. R., Zhou, W., Jakovčevski, I., Zečević, N., & Antic, S. D. (2012) · Neuromethods · doi:10.1007/978-1-62703-020-5_3 · Cited by 13
Schutte, R. J., Xie, Y., Ng, N. N., Figueroa, P., Pham, A. T., & O’Dowd, D. K. (2017) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2017.07.019 · Cited by 13
Benazzato, C., Lojudice, F. H., Pöehlchen, F., Leite, P. E. C., Manucci, A. C., van der Linden, V., Jungmann, P., Sogayar, M. C., Bruni‐Cardoso, A., Russo, F. B., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-65392-8 · Cited by 14
Simulation of developing human neuronal cell networks
Lenk, K., Priwitzer, B., Ylä‐Outinen, L., Tietz, L. H. B., Narkilahti, S., & Hyttinen, J. (2016) · BioMedical Engineering OnLine · doi:10.1186/s12938-016-0226-6 · Cited by 12
Berryer, M. H., Rizki, G., Nathanson, A. J., Klein, J. A., Trendafilova, D., Susco, S. G., Lam, D., Messana, A., Holton, K. M., Karhohs, K. W., et al. (2023) · eLife · doi:10.7554/elife.80168 · Cited by 14
Buchholz, S., Bell, M. J., Cakmak, C., Klimek, J., Gan, L., & Zempel, H. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-3629-9_31 · Cited by 12
Broccoli, V., Rubio, A., Taverna, S., & Yekhlef, L. (2015) · Experimental Biology and Medicine · doi:10.1177/1535370215577585 · Cited by 11
Electrophysiological properties of neurons derived from human stem cells and iNeurons in vitro
Halliwell, R. F. (2016) · Neurochemistry International · doi:10.1016/j.neuint.2016.10.003 · Cited by 11
Embryo experimentation: is there a case for moving beyond the ‘14-day rule’
Castelyn, G. (2020) · Bioethics News · doi:10.1007/s40592-020-00117-x · Cited by 11
Kraus, S., Lehner, B., Reichhart, N., Couillard‐Després, S., Wagner, K., Bogdahn, U., Aigner, L., & Strauß, O. (2013) · Glia · doi:10.1002/glia.22551 · Cited by 12
Neural Differentiation of Human Embryonic Stem Cells at the Ultrastructural Level
Mumaw, J. L., Machacek, D. W., Shields, J. P., Dodla, M. C., Dhara, S. K., & Stice, S. L. (2010) · Microscopy and Microanalysis · doi:10.1017/s1431927609991279 · Cited by 11
Park, J., Lim, M.-S., So yeon, J. I., Cho, M. S., Park, S.-J., Han, S., & Kim, J.-H. (2017) · Journal of Anesthesia · doi:10.1007/s00540-017-2408-1 · Cited by 11
Zeidler, M., Kummer, K. K., & Kress, M. (2022) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-022-02707-6 · Cited by 11
Jezierski, A., Baumann, E., Aylsworth, A., Costain, W. J., Corluka, S., Banderali, U., Sodja, C., Ribecco‐Lutkiewicz, M., Alasmar, S., Martina, M., et al. (2021) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10263-2 · Cited by 10
Lai, B., Mao, X. O., Greenberg, D. A., & Jin, K. (2008) · Stem Cells and Development · doi:10.1089/scd.2007.0124 · Cited by 10
Molecular Mechanisms of Astrocyte Involvement in Synaptogenesis and Brain Synaptic Plasticity
Хаспеков, Л. Г., & Frumkina, L. E. (2023) · Biochemistry (Moscow) · doi:10.1134/s0006297923040065 · Cited by 10
Verma, I., Rashid, Z., Sikdar, S. K., & Seshagiri, P. B. (2017) · International Journal of Developmental Neuroscience · doi:10.1016/j.ijdevneu.2017.06.009 · Cited by 9
Ori, A. P. S., Bot, M. H. M., Molenhuis, R. T., Loohuis, L. M. O., & Ophoff, R. A. (2018) · Biological Psychiatry · doi:10.1016/j.biopsych.2018.08.019 · Cited by 10
Replicable Expansion and Differentiation of Neural Precursors from Adult Canine Skin
Duncan, T., Lowe, A., Sidhu, K., Sachdev, P. S., Lewis, T., Lin, R. C. Y., Sytnyk, V., & Valenzuela, M. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.07.008 · Cited by 9
Modelling neurodegenerative diseases in vitro: Recent advances in 3D iPSC technologies
Siney, E. J., Kurbatskaya, K., Chatterjee, S., Prasannan, P., Mudher, A. K., & Willaime‐Morawek, S. (2018) · AIMS Cell and Tissue Engineering · doi:10.3934/celltissue.2018.1.1 · Cited by 9
Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation
Kim, T. H. L., Koo, B. I., Yun, J. S., Cho, S., & Nam, Y. S. (2019) · ACS Omega · doi:10.1021/acsomega.9b00998 · Cited by 9
Weick, J. P., Kang, H., Bonadurer, G. F., & Bhattacharyya, A. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2627-5_15 · Cited by 9
Self-citationEmerging model in anesthetic developmental neurotoxicity: human stem cells.
Bai, X., & Bosnjak, Z. J. (2013) · PubMed · Cited by 7
Waxman, E. A., Dungan, L. V., DeFlitch, L., Merchant, J. P., Gagne, A. L., Goldberg, E. M., & French, D. L. (2023) · Current Protocols · doi:10.1002/cpz1.948 · Cited by 7
Chen, C. Y., Plocik, A. M., Anderson, N. C., Moakley, D. F., Boyi, T., Dundes, C. E., Lassiter, C. M., Graveley, B. R., & Grabel, L. B. (2016) · Stem Cell Reviews and Reports · doi:10.1007/s12015-016-9676-2 · Cited by 8
Niu, D., Wang, L., Cui, J., Zhou, B., & Yao, L. (2019) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2019.172737 · Cited by 8
Arnst, N., Mairal, P. B., García‐González, L., Arnaud, L., Greetham, L., Nivet, E., Rivera, S., & Dityatev, A. E. (2021) · Cells · doi:10.3390/cells10071705 · Cited by 8
Can Astrocytes Be a Target for Precision Medicine?
Allen, C. F., Shaw, P. J., & Ferraiuolo, L. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-60733-7_7 · Cited by 7
Schick, R., Farah, N., Markus, A., Korngreen, A., & Mandel, Y. (2020) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.61.11.44 · Cited by 6
Chae, W. R., & Lee, N. Y. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01589j · Cited by 6
Using 2D and 3D pluripotent stem cell models to study neurotropic viruses
LaNoce, E., Dumeng-Rodriguez, J., & Christian, K. M. (2022) · Frontiers in Virology · doi:10.3389/fviro.2022.869657 · Cited by 7
Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neurons
Prytkova, I., Liu, Y., Fernando, M. B., Gameiro‐Ros, I., Popova, D., Kamarajan, C., Xuei, X., Chorlian, D. B., Edenberg, H. J., Tischfield, J. A., et al. (2024) · Journal of Neuroscience · doi:10.1523/jneurosci.0918-23.2024 · Cited by 6
Concepts toward directing human astroplasticity to promote neuroregeneration
Patel, R., Muir, M. T., Cvetkovic, C., & Krencik, R. (2018) · Developmental Dynamics · doi:10.1002/dvdy.24655 · Cited by 5
Risher, W. C., & Eroğlu, Ç. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-823672-7.00003-x · Cited by 6
Cheng, Y.-C., Huang, C.-J., Ku, W., Guo, S.-L., Tien, L.-T., Lee, Y.-J., & Chien, C.-C. (2022) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10319-3 · Cited by 6
Characterization of Ionic Currents in Human Neural Stem Cells
Lim, C. G., Kim, S., Suh‐Kim, H., Lee, Y., & Ahn, S. C. (2008) · Korean Journal of Physiology and Pharmacology · doi:10.4196/kjpp.2008.12.4.131 · Cited by 6
Buchholz, S., Bell, M. J., Kabbani, M. A. A., Kluge, L., Cagkmak, C., Klimek, J., & Zempel, H. (2022) · Research Square · doi:10.21203/rs.3.rs-2277268/v1 · Cited by 6
Shimba, K., Kotani, K., & Jimbo, Y. (2021) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2021.3078473 · Cited by 5
Glial Control of Synaptogenesis
Allen, N. J. (2012) · Oxford University Press eBooks · doi:10.1093/med/9780199794591.003.0031 · Cited by 4
Gu, J., Rollo, B., Berecki, G., Petrou, S., Kwan, P., Sümer, H., & Cromer, B. A. (2023) · Experimental Cell Research · doi:10.1016/j.yexcr.2023.113902 · Cited by 5
Telencephalic Neurogenesis Versus Telencephalic Differentiation of Pluripotent Stem Cells
Nat, R., Apostolova, G., & Dechant, G. (2013) · InTech eBooks · doi:10.5772/54251 · Cited by 7
Sullivan, M. A., Lane, S. D., Volkerling, A. M., Engel, M., Werry, E. L., & Kassiou, M. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.22541/au.166852614.45511209/v1 · Cited by 5
Sex Hormones and Dream Frequency
Tkemaladze, J. (2026) · Longevity Horizon · doi:10.65649/1fgxy298 · Cited by 4
Verma, I., & Seshagiri, P. B. (2025) · Stem Cell Reviews and Reports · doi:10.1007/s12015-025-10851-6 · Cited by 4
Kesavan, J., Wang, Y., Dinkel, K., Hamacher, M., Prehn, J. H. M., Henshall, D. C., & Engel, T. (2025) · British Journal of Pharmacology · doi:10.1111/bph.70167 · Cited by 4
Nodule Detection in Postero Anterior Chest Radiographs
Campadelli, P., & Casiraghi, E. (2004) · Lecture notes in computer science · doi:10.1007/978-3-540-30136-3_132 · Cited by 4
Hong, J., Dragas, R., Khazaei, M., Ahuja, C. S., & Fehlings, M. G. (2021) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2021.741681 · Cited by 3
Salmanzadeh, H., Poojari, A., Rabiee, A., Zeitlin, B. D., & Halliwell, R. F. (2023) · Frontiers in Neuroscience · doi:10.3389/fnins.2023.1182720 · Cited by 3
Kim, S., Kim, C., Moon, H. W., Han, J., Choi, S. W., Park, S. S., Kim, H., Choi, B., Kang, J. Y., Kim, J., et al. (2025) · Biomaterials Science · doi:10.1039/d5bm00535c · Cited by 3
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Goh, E., & Chin, E. W. M. (2015) · Neural Regeneration Research · doi:10.4103/1673-5374.169607 · Cited by 3
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Smith, A. S. T., Long, C. J., McAleer, C. W., Guo, X., Esch, M. B., Prot, J. M., Shuler, M. L., & Hickman, J. J. (2014) · Royal Society of Chemistry eBooks · doi:10.1039/9781782620136-00132 · Cited by 3
Synaptic signalling in a network of dopamine neurons: what prevents proper intercellular crosstalk?
Chen, Y., Kunath, T., Simpson, J., Homer, N. Z. M., & Sylantyev, S. (2020) · FEBS Letters · doi:10.1002/1873-3468.13910 · Cited by 3
Shi, Y., van Rhijn, J. R., Bormann, M., Mossink, B., Frega, M., Hakobjan, M., Kittel‐Schneider, S., Schubert, D., Brunner, H. G., Franke, B., et al. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/741108 · Cited by 3
Chikuchi, R., Kato, Y., Tomatsu, A., Nishisaki, S., Kawakami, Y., Yoshimura, T., Li, J., Iguchi, Y., Onodera, K., Hashimoto, R., et al. (2025) · Neuroscience Research · doi:10.1016/j.neures.2025.105003 · Cited by 2
Induction of Neural Lineages from Mesoderm and Endoderm by Defined Transcription Factors
Wernig, M. (2013) · Research and perspectives in neurosciences · doi:10.1007/978-3-642-36648-2_3 · Cited by 1
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Pio, T., Hill, E. J., Kebede, N., Andersen, J., & Sloan, S. A. (2024) · Advances in neurobiology · doi:10.1007/978-3-031-64839-7_4 · Cited by 2
Dimitrov, D., Lien, Y., Hori, T., Goda, Y., Rosenmund, C., & Taoufiq, Z. (2025) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102604 · Cited by 3
Neuron-Astroglial Interactions in Cell Fate Commitment in the Central Nervous System
Stipursky, J., Spohr, T. C. L. D. S. E., Romão, L. F., & Gomes, F. C. A. (2009) · Springer eBooks · doi:10.1007/978-90-481-3375-8_11 · Cited by 2
BDNF expression is up-regulated by progesterone in human umbilical cord mesenchymal stem cells
Yang, J., Wang, X., Liu, S., & Xue, G. (2016) · Neurological Research · doi:10.1080/01616412.2016.1235248 · Cited by 2
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Batenburg, K. L., Rohde, S. K., Cornelissen‐Steijger, P., Breeuwsma, N., Heine, V. M., & Scheper, W. (2023) · Current Protocols · doi:10.1002/cpz1.900 · Cited by 2
Engineering circuits of human iPSC-derived neurons and rat primary gliaPreprint
Girardin, S., Ihle, S. J., Menghini, A., Krubner, M., Tognola, L., Duru, J., Ruff, T., Fruh, I., Müller, M., & Vörös, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.11.07.515431 · Cited by 2
Yao, C., Jin, L., Zhong, J., Huang, Q., Bao, Z., Zhou, S., Wang, C., Li, H., Yuan, X., Wang, Z., et al. (2025) · Cell Proliferation · doi:10.1111/cpr.70093 · Cited by 1
Jones, E. N. B., Sakaguchi, D. S., & Mallapragada, S. K. (2009) · Springer eBooks · doi:10.1007/978-0-387-98161-1_12 · Cited by 1
In Vitro Modeling of Nervous System: Engineering of the Reflex Arc
Guo, X., Sommerhage, F., McAleer, C. W., Martin, C., Long, C. J., Wang, Y. I., Santhanam, N., Colón, A., Oleaga, C., & Hickman, J. J. (2016) · Springer eBooks · doi:10.1007/978-3-319-31433-4_9 · Cited by 1
Fast and Efficient Neural Conversion of Human Hematopoietic Cells
Bruzos-Cidón, C., Castaño, J., Torrecilla, M., Sánchez‐Pernaute, R., & Giorgetti, A. (2016) · Current Protocols in Stem Cell Biology · doi:10.1002/cpsc.16 · Cited by 1
Gramatiuk, S. M., Іvanova, Y. V., & Hudyma, А. А. (2020) · Klinicheskaia khirurgiia · doi:10.26779/2522-1396.2020.9-10.68 · Cited by 1
Deleanu, R. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.96653 · Cited by 1
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Metadata
- Authors
- Johnson, M. A., Weick, J. P., Pearce, R. A., & Zhang, S.-C.
- Affiliations
- University of Wisconsin System (US)
- University of Wisconsin–Madison (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2007-03-21
- Volume
- 27
- Issue
- 12
- Pages
- 3069-3077
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC2735200
- OpenAlex
- W2016961314
- Cited by
- 318
- References
- 69
- Citations by year
- 2026: 2 · 2025: 11 · 2024: 6 · 2023: 12 · 2022: 16 · 2021: 16 · 2020: 17 · 2019: 21 · 2018: 19 · 2017: 24 · 2016: 22 · 2015: 21 · 2014: 25 · 2013: 16 · 2012: 17
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Waisman Center (P30 HD03352, HD03352)
- National Institutes of Health (HD033.52)
- National Institute of General Medical Sciences (T32GM008692)
- National Institute of Neurological Disorders and Stroke (R01NS045926)
- National Institute of Child Health and Human Development (P30HD03352, HD03352)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P30HD03352., P30HD003352)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- human embryonic stem cells, neuronal differentiation, neuroepithelial cells, synaptic activity, action potential generation, sodium and potassium currents, resting membrane potential, spontaneous synaptic transmission, astrocyte-conditioned medium, synaptic protein localization, neuronal maturation
- MeSH terms
- Action Potentials, Animals, Astrocytes, Cell Differentiation, Cells, Cultured, Humans, Synaptic Transmission, Neurons, Coculture Techniques, Cell Enlargement, Cell Proliferation, Mice, Embryonic Stem Cells
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