The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Structural and functional analysis of the GABARAP interaction motif (GIM)
Open accessAbstract
Through the canonical LC3 interaction motif (LIR), [W/F/Y]‐X 1 ‐X 2 ‐[I/L/V], protein complexes are recruited to autophagosomes to perform their functions as either autophagy adaptors or receptors. How these adaptors/receptors selectively interact with either LC3 or GABARAP families remains unclear. Herein, we determine the range of selectivity of 30 known core LIR motifs towards individual LC3s and GABARAPs. From these, we define a G ABARAP I nteraction M otif (GIM) sequence ([W/F]‐[V/I]‐X 2 ‐V) that the adaptor protein PLEKHM1 tightly conforms to. Using biophysical and structural approaches, we show that the PLEKHM1‐LIR is indeed 11‐fold more specific for GABARAP than LC3B. Selective mutation of the X 1 and X 2 positions either completely abolished the interaction with all LC3 and GABARAPs or increased PLEKHM1‐GIM selectivity 20‐fold towards LC3B. Finally, we show that conversion of p62/SQSTM1, FUNDC1 and FIP200 LIRs into our newly defined GIM, by introducing two valine residues, enhances their interaction with endogenous GABARAP over LC3B. The identification of a GABARAP‐specific interaction motif will aid the identification and characterization of the expanding array of autophagy receptor and adaptor proteins and their in vivo functions.
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Cites
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BackgroundQuantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy
Mancias, J. D., Wang, X., Gygi, S. P., Harper, J. W., & Kimmelman, A. C. (2014) · Nature · doi:10.1038/nature13148 · Cited by 2,084
Nix is a selective autophagy receptor for mitochondrial clearance
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Cargo recognition and trafficking in selective autophagy
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Self-citationRegulation of endoplasmic reticulum turnover by selective autophagy
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Background
Cited by
Autophagy and autophagy-related proteins in cancer
Li, X., He, S., & Ma, B. (2020) · Molecular Cancer · doi:10.1186/s12943-020-1138-4 · Cited by 1,613
A brief history of autophagy from cell biology to physiology and disease
Mizushima, N. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0092-5 · Cited by 696
Selective autophagy of intracellular organelles: Recent research advances
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Diverse Cellular Roles of Autophagy
Morishita, H., & Mizushima, N. (2019) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100818-125300 · Cited by 376
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy
Chen, Q., Xiao, Y., Chai, P., Zheng, P., Teng, J., & Chen, J. (2019) · Current Biology · doi:10.1016/j.cub.2019.01.041 · Cited by 304
Metadata
- Authors
- Rogov, V. V., Stolz, A., Ravichandran, A. C., Rios‐Szwed, D. O., Suzuki, H., Kniss, A., Löhr, F., Wakatsuki, S., Dötsch, V., Dikic, I., Dobson, R. C., & McEwan, D. G.
- Venue
- EMBO reports
- Published
- 2017-06-27
- Volume
- 18
- Issue
- 8
- Pages
- 1382-1396
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2540740602
- Cited by
- 174
- References
- 9
- FWCI
- 14.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Rogov, V. V., Stolz, A., Ravichandran, A. C., Rios‐Szwed, D. O., Suzuki, H., Kniss, A., Löhr, F., Wakatsuki, S., Dötsch, V., Dikic, I., Dobson, R. C., & McEwan, D. G. (2017). Structural and functional analysis of the GABARAP interaction motif (GIM). EMBO reports. https://doi.org/10.15252/embr.201643587
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