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Cited by 226Bacterial Genetics and BiotechnologyCell Image Analysis TechniquesAdvanced Fluorescence Microscopy Techniques

The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.

Cell Shape Dynamics in Escherichia coli

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Reshes, Galina, Vanounou, Sharon, Fishov, Itzhak, Feingold, Mario·2008·Biophysical Journal
DOI 10.1529/biophysj.107.104398PMC PMC2134870
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Abstract

Bacteria are the simplest living organisms. In particular, Escherichia coli has been extensively studied and it has become one of the standard model systems in microbiology. However, optical microscopy studies of single E. coli have been limited by its small size, approximately 1 x 3 microm, not much larger than the optical resolution, approximately 0.25 microm. As a result, not enough quantitative dynamical information on the life cycle of single E. coli is presently available. We suggest that, by careful analysis of images from phase contrast and fluorescence time-lapse microscopy, this limitation can be bypassed. For example, we show that applying this approach to monitoring morphogenesis in individual E. coli leads to a simple, quantitative description of this process. First, we find the time when the formation of the septum starts, tau(c). It occurs much earlier than the time when the constriction can be directly observed by phase contrast. Second, we find that the growth law of single cells is more likely bilinear/trilinear than exponential. This is further supported by the relations that hold between the corresponding growth rates. These methods could be further extended to study the dynamics of cell components, e.g., the nucleoid and the Z-ring.

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Crossref · 10.1529/biophysj.107.104398

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Citations

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Research works printed but not linked: 1

  1. Marr, 1969, Growth and division of individual bacteria, Symp. Soc. Gen. Microbiol., 19, 237

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Metadata

Authors
Reshes, G., Vanounou, S., Fishov, I., & Feingold, M.
Affiliations
Ben-Gurion University of the Negev (IL)
Corresponding author
Fishov, Itzhak
ORCID
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
2008-01
Volume
94
Issue
1
Pages
251-264
ISSN
0006-3495 (ISSN-L)
DOI
10.1529/biophysj.107.104398
PMC
PMC2134870
OpenAlex
W2025598949
Cited by
226
References
82
Citations by year
2026: 12 · 2025: 9 · 2024: 16 · 2023: 9 · 2022: 13 · 2021: 20 · 2020: 27 · 2019: 17 · 2018: 11 · 2017: 14 · 2016: 17 · 2015: 10 · 2014: 11 · 2013: 8 · 2012: 4
FWCI
3.94
Type
journal article
Language
en
Open access
Licence
Locations
  • Biophysical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Israel Academy of Sciences and Humanities (820/05)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
Escherichia coli, cell shape dynamics, bacterial morphogenesis, cell division, septum formation, cell constriction, single-cell imaging, time-lapse microscopy, phase contrast microscopy, fluorescence microscopy, Z-ring, nucleoid
MeSH terms
Computer Simulation, Escherichia coli, Image Interpretation, Computer-Assisted, Models, Biological, Cell Enlargement

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