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Cited by 49Regulation of Appetite and ObesityAdipokines, Inflammation, and Metabolic DiseasesGrowth Hormone and Insulin-like Growth Factors

Expression of ghrelin gene in peripheral blood mononuclear cells and plasma ghrelin concentrations in patients with metabolic syndrome.

Mager, Ursula, Kolehmainen, Marjukka, de Mello, Vanessa D F, Schwab, Ursula, Laaksonen, David E, Rauramaa, Rainer, Gylling, Helena, Atalay, Mustafa +2 more·2008·European Journal of Endocrinology
DOI 10.1530/eje-07-0862
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Abstract

Objective: We examined the expression of ghrelin and ghrelin receptors in peripheral blood mononuclear cells (PBMCs) and evaluated the effect of weight loss or exercise on plasma ghrelin concentrations in subjects with the metabolic syndrome. Design and methods: Data from 75 overweight/obese subjects randomized to a weight loss, aerobic exercise, resistance exercise or control group for a 33-week intervention period were analysed. The plasma ghrelin concentrations and indices of insulin and glucose metabolism were assessed, and mRNA expression of ghrelin, its receptors and various cytokines in PBMCs was studied using real-time PCR. Results: Ghrelin and GH secretagogue receptor 1b were expressed in PBMCs of subjects with metabolic syndrome. Ghrelin gene expression correlated positively with the expressions of tumour necrosis factor-α (P<0.001), interleukin-1β (P<0.001) and interleukin-6 (P=0.026) during the study, but was not associated with the plasma ghrelin concentration. Genotype-specific ghrelin gene expression in PBMCs was found for the −604G/A and the −501A/C polymorphisms in the ghrelin gene. At baseline, the plasma ghrelin levels were associated with fasting serum insulin concentrations, insulin sensitivity index and high-density lipoprotein cholesterol. However, longitudinally weight, BMI or waist circumference and acute insulin response in i.v. glucose tolerance test were stronger predictors of the ghrelin concentration. Plasma ghrelin did not change over the study period in the weight reduction group, but it tended to decrease in the control group (P=0.050). Conclusions: Ghrelin mRNA expression in PBMCs suggests an autocrine role for ghrelin within an immune microenvironment. Moderate long-term weight loss may prevent a decline in ghrelin concentration over time in individuals with metabolic syndrome.

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Authors
Mager, U., Kolehmainen, M., de Mello, V. D. F., Schwab, U., Laaksonen, D. E., Rauramaa, R., Gylling, H., Atalay, M., Pulkkinen, L., & Uusitupa, M.
Affiliations
  • Kuopio University Hospital (FI)
  • University of Eastern Finland (FI)
Corresponding author
Mager, Ursula
ORCID
Venue
European Journal of Endocrinology
Publisher
Oxford University Press (OUP)
Published
2008-04
Volume
158
Issue
4
Pages
499-510
ISSN
  • 0804-4643 (ISSN-L)
  • 1479-683X
DOI
10.1530/eje-07-0862
OpenAlex
W2018685387
Cited by
49
References
71
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2021: 1 · 2019: 1 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 5
FWCI
2.82
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Österreichischen Akademie der Wissenschaften
  • Suomen Kulttuurirahasto
  • Juho Vainion Säätiö
  • Kuopion Yliopistollinen Sairaala
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
ghrelin, growth hormone secretagogue receptor, metabolic syndrome, peripheral blood mononuclear cells, TNF-α, IL-1β, interleukin-6, weight loss, aerobic exercise, resistance training, insulin sensitivity, obesity
MeSH terms
Adult, Aged, Body Weights and Measures, Exercise Therapy, Female, Genotype, Humans, Leukocytes, Mononuclear, Male, Middle Aged, Osmolar Concentration, Research Design, Weight Loss, Gene Expression, Inflammation Mediators, Autocrine Communication, Polymorphism, Single Nucleotide, Metabolic Syndrome, Ghrelin, Receptors, Ghrelin

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