Immunoreactive cytochrome P-45017α in rat and guineapig gonads, adrenal glands and brain
Abstract
The cytochrome P-450(17 alpha)-hydroxylase, 17----20 lyase (P-450(17 alpha)) is the key enzyme responsible for the biosynthesis of androgens in steroidogenic organs. Its cellular localization has been examined with an immunohistochemical technique. In immature rat ovary, P-450(17 alpha) was first detected in sparse interstitial cells on postnatal Day 8. The number of immunoreactive interstitial cells increased thereafter and the intensity of P-450(17 alpha) staining in these cells was highest at 3 weeks of age. The intensity of staining then started to decline and was very faint at Day 35. From 6 weeks on, the distribution of immunoreactive P-450(17 alpha) was of the adult type: it was detected exclusively in the thecal cells of the large antral, preovulatory, follicles. P-450(17 alpha) was not detectable during pregnancy except on the day of parturition, when thecal cells were transiently immunoreactive. The staining had vanished 24 h after delivery. Human chorionic gonadotrophin (hCG), injected into immature females on Days 24 to 26, induced P-450(17 alpha) prematurely in thecal cells. When injected on Days 12 to 14 of pregnancy, hCG also induced P-450(17 alpha) in the thecal cells surrounding the largest follicles, whereas the interstitial and luteal cells were not immunostained. The antiprogestin RU486, injected on Day 16 of pregnancy, reinstated P-450(17 alpha) (and P-450scc) immunoreactivity in the thecal cells. Oestradiol selectively suppressed P-450(17 alpha) expression in the thecal cells of RU486-treated females. In immature guinea-pig ovary, P-450(17 alpha) was immunostained in thecal cells, not in interstitial cells, although the interstitial cells expressed the delta 5-3 beta-hydroxysteroid dehydrogenase. P-450(17 alpha) was also immunolocalized in the Leydig cells of rat and guinea-pig testes, and in the guinea-pig adrenal cortex (zonae fasciculata and reticularis), but not in the rat adrenal cortex. P-450(17 alpha) was not detectable in the brain of either rat or guinea-pig.
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The expression of CYP17A1 in adrenal of adult mice and rat is minimal.
“Importantly, the expression of CYP17A1 in adrenal of adult mice and rat, which are frequently used as pre-clinical models for endocrine and pharmacology studies, is minimal (Le Goascogne et al., 1991, Mostaghel et al., 2017, Rodriguez et al., 1997).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Le Goascogne et al., 1991
Other rodents such as hamster and guinea pig do produce adrenal C19 steroids due to adrenal CYP17A1 expression, but CYP17A1 substrate specificity in these species is significantly different from that of the human enzyme, thus limiting comparisons to human adrenal steroidogenesis.
“Furthermore, while other rodents such as hamster (Cloutier et al., 1995) and guinea pig (Le Goascogne et al., 1991, Tremblay et al., 1995) do produce adrenal C19 steroids due to adrenal CYP17A1 expression, CYP17A1 substrate specificity in these species is significantly different from that of the human enzyme (Cloutier et al., 1997, Tremblay et al., 1995), thus limiting comparisons to human adrenal”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Le Goascogne et al., 1991
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- Authors
- Le Goascogne, C., Sananés, N., Gouézou, M., Takemori, S., Kominami, S., Baulieu, E. E., & Robel, P.
- Affiliations
- Université Paris-Sud (FR)
- Hiroshima University (JP)
- ORCID
- Baulieu, E. E.
- Venue
- Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-11-01
- Volume
- 93
- Issue
- 2
- Pages
- 609-622
- ISSN
- 1470-1626 (ISSN-L)
- 1741-7899
- OpenAlex
- W2079904803
- Cited by
- 113
- References
- 17
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 4 · 2018: 8 · 2017: 4 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- androgen production, ovary, testis, adrenal gland, brain, theca cells, interstitial cells, Leydig cells, human chorionic gonadotropin, RU486, estradiol, pregnancy, postnatal development
- MeSH terms
- Adrenal Cortex, Animals, Brain, Estradiol, Female, Chorionic Gonadotropin, Guinea Pigs, Immunohistochemistry, Leydig Cells, Male, Ovary, Pregnancy, Rats, Inbred Strains, Sexual Maturation, Steroid 17-alpha-Hydroxylase, Testis, Theca Cells, Mifepristone, Rats
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