The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors
Open accessAbstract
Summary. Cells isolated from the ejaculates of a high proportion of patients exhibiting oligozoospermia are characterized by generation rates of reactive oxygen species that considerably exceed those obtained for the normal fertile population. The purpose of this study was to resolve the cellular source of this enhanced activity. Semen samples from a cohort of oligozoospermic patients and a group of fertile controls were fractionated on discontinuous Percoll gradients to generate three cell populations (0, 50 and 100%) of differing density. For each fraction, both the steady-state and the phorbolester-induced chemiluminescent signals were significantly (P < 0·001) greater for the oligozoospermic samples than for the fertile controls. In the fertile donors, leucocytes comprised the major source of reactive oxygen species, particularly in the low-density Percoll fractions; in oligozoospermic patients, however, spermatozoa were identified as a second major source of reactive oxygen species. Particularly striking was an intense phorbol-ester-induced chemiluminescent signal generated by oligozoospermic spermatozoa, purified by passage through isotonic Percoll and free of leucocyte contamination, which was 167 times greater than the median signal generated by the corresponding fraction from the fertile controls (P < 0·001). These results emphasize the importance of spermatozoa as a major source of reactive oxygen species in oligozoospermia and have implications for the diagnosis and treatment of this condition, as well as for the design of appropriate diagnostic strategies. Keywords: oligozoospermia; reactive oxygen species; spermatozoa; human
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Citations
Cites
Generation of Reactive Oxygen Species, Lipid Peroxidation, and Human Sperm Function
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Self-citationThe culture of mouse embryos in vitro.
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Self-citationAitken, R. J., Irvine, D. S., & Wu, F. C. (1991) · American Journal of Obstetrics and Gynecology · doi:10.1016/s0002-9378(11)80017-7 · Cited by 374
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What do we measure by a luminol-dependent chemiluminescence of phagocytes?
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Cited by
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Self-citationCauses and consequences of oxidative stress in spermatozoa
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Metadata
- Authors
- Aitken, R. J., Buckingham, D., West, K., Wu, F. C., Zikopoulos, K., & Richardson, D. W.
- Venue
- Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-03-01
- Volume
- 94
- Issue
- 2
- Pages
- 451-462
- ISSN
- 1470-1626 (ISSN-L)
- 1741-7899
- OpenAlex
- W1977544074
- Cited by
- 269
- References
- 12
- FWCI
- 9.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors. https://app.cochat.ai/papers/10.1530/jrf.0.0940451
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- Aitken, R. J., Buckingham, D., West, K., Wu, F. C., Zikopoulos, K., & Richardson, D. W. (1992). Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors. Reproduction. https://doi.org/10.1530/jrf.0.0940451
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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