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Stacks: Building and Genotyping Loci De Novo From Short-Read Sequences

Open access
Catchen, Julian M, Amores, Angel, Hohenlohe, Paul, Cresko, William, Postlethwait, John H·2011·G3 Genes|Genomes|Genetics

Abstract

Advances in sequencing technology provide special opportunities for genotyping individuals with speed and thrift, but the lack of software to automate the calling of tens of thousands of genotypes over hundreds of individuals has hindered progress. Stacks is a software system that uses short-read sequence data to identify and genotype loci in a set of individuals either de novo or by comparison to a reference genome. From reduced representation Illumina sequence data, such as RAD-tags, Stacks can recover thousands of single nucleotide polymorphism (SNP) markers useful for the genetic analysis of crosses or populations. Stacks can generate markers for ultra-dense genetic linkage maps, facilitate the examination of population phylogeography, and help in reference genome assembly. We report here the algorithms implemented in Stacks and demonstrate their efficacy by constructing loci from simulated RAD-tags taken from the stickleback reference genome and by recapitulating and improving a genetic map of the zebrafish, Danio rerio.

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Metadata

Authors
Catchen, J. M., Amores, A., Hohenlohe, P., Cresko, W., & Postlethwait, J. H.
Venue
G3 Genes|Genomes|Genetics
Published
2011-08-01
Volume
1
Issue
3
Pages
171-182
ISSN
2160-1836 (ISSN-L)
OpenAlex
W2161901637
Cited by
1,932
References
6
FWCI
19.63
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Stacks: Building and Genotyping Loci De Novo From Short-Read Sequences. https://app.cochat.ai/papers/10.1534/g3.111.000240
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Catchen, J. M., Amores, A., Hohenlohe, P., Cresko, W., & Postlethwait, J. H. (2011). Stacks: Building and Genotyping Loci De Novo From Short-Read Sequences. G3 Genes|Genomes|Genetics. https://doi.org/10.1534/g3.111.000240

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