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Cited by 19Chromosomal and Genetic VariationsGenomics and Chromatin DynamicsNuclear Structure and Function

Regulation of Nucleolar Dominance in Drosophila melanogaster

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Warsinger-Pepe, Natalie, Li, Duojia, Yamashita, Yukiko M·2020·Genetics
DOI 10.1534/genetics.119.302471PMC PMC7153946

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Abstract

In eukaryotic genomes, ribosomal RNA (rRNA) genes exist as tandemly repeated clusters, forming ribosomal DNA (rDNA) loci. Each rDNA locus typically contains hundreds of rRNA genes to meet the high demand of ribosome biogenesis. Nucleolar dominance is a phenomenon whereby individual rDNA loci are entirely silenced or transcribed, and is believed to be a mechanism to control rRNA dosage. Nucleolar dominance was originally noted to occur in interspecies hybrids, and has been shown to occur within a species (i.e., nonhybrid context). However, studying nucleolar dominance within a species has been challenging due to the highly homogenous sequence across rDNA loci. By utilizing single nucleotide polymorphisms between X rDNA and Y rDNA loci in males, as well as sequence variations between two X rDNA loci in females, we conducted a thorough characterization of nucleolar dominance throughout development of Drosophila melanogaster. We demonstrate that nucleolar dominance is a developmentally regulated program that occurs in nonhybrid, wild-type D. melanogaster, where Y rDNA dominance is established during male embryogenesis, whereas females normally do not exhibit dominance between two X rDNA loci. By utilizing various chromosomal complements (e.g., X/Y, X/X, X/X/Y) and a chromosome rearrangement, we show that the short arm of the Y chromosome including the Y rDNA likely contains information that instructs the state of nucleolar dominance. Our study begins to reveal the mechanisms underlying the selection of rDNA loci for activation/silencing in nucleolar dominance in the context of nonhybrid D. melanogaster.

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Cited by

Sorted by citations

Authors
Warsinger-Pepe, N., Li, D., & Yamashita, Y. M.
Affiliations
  • University of Michigan (US)
  • Howard Hughes Medical Institute (US)
Corresponding author
Yamashita, Yukiko M
ORCID
Venue
Genetics
Publisher
Oxford University Press (OUP)
Published
2020-04-01
Volume
214
Issue
4
Pages
991-1004
ISSN
  • 0016-6731 (ISSN-L)
  • 1943-2631
DOI
10.1534/genetics.119.302471
PMC
PMC7153946
OpenAlex
W3009249389
Cited by
19
References
2
Citations by year
2025: 3 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 2 · 2020: 1
FWCI
2.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Genetics (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health
  • University of California, San Diego
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD079342)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
nucleolar dominance, ribosomal DNA, rDNA silencing, Y chromosome, embryonic development, single nucleotide polymorphisms, chromosomal rearrangements, ribosome biogenesis, Drosophila melanogaster
MeSH terms
Animals, Cell Nucleolus, Drosophila melanogaster, Female, Genes, Dominant, Male, RNA, Ribosomal, Sex Chromosomes, Spermatogonia, Gene Expression Regulation, Developmental, Polymorphism, Single Nucleotide, Chromosomes, Insect

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