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Temperature, pH and carbon source affect drastically indole acetic acid production of plant growth promoting yeasts

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Scarcella, Ana Silvia de Almeida, Bizarria Junior, Rodolfo, Bastos, Reinaldo Gaspar, Magri, Márcia Maria Rosa·2017·Brazilian Journal of Chemical Engineering
DOI 10.1590/0104-6632.20170342s20150541
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Abstract

Rhizosphere and phylloplane yeasts are able to produce phytohormones, acting as potential plant growth promoters. The aim of this study was to analyze the production of indole acetic acid (IAA) by yeasts using variations in the culture conditions. The yeasts evaluated were Rhodotorula mucilaginosa and Trichosporon asahii, which were cultivated in a potato broth medium with 20 g/L of glucose or sucrose as the carbon source. The cultivation was carried out at 22°C, 25°C or 30°C, and the initial pH was adjusted to 3.0, 4.5 or 6.0. The cultures were analyzed using a colorimetric technique with Salkowski reagent. The cell count was determined in all of the conditions. The results for T. asahii showed that IAA production with sucrose was superior at pH 6.0, and that with glucose was superior at pH 4.5. These results indicate an association between the carbon source and medium pH. Rh. mucilaginosa (2F32) presented a high IAA production when compared with T. asahii (3S44 and 4C06) or even with other microorganisms referenced in the literature. The highest production (0.655 g/L) was obtained at pH 6.0 with glucose as the carbon source. Variations in the culture conditions did not affect the cell growth of the yeasts.

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Figure 1 . Figure 1. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (Cstrain 2F32) at diff erent medium pH (♦-pH 3.0; ■ -pH 4.5; ▲-pH 6.0). Cultures were incubated in a shaker (160 rpm) at 25ºC, with 2% glucose as the carbon source. Data was the means of three replicates. Bars at each point indicate ± SE.
Figure 1 . Figure 1. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (Cstrain 2F32) at diff erent medium pH (♦-pH 3.0; ■ -pH 4.5; ▲-pH 6.0). Cultures were incubated in a shaker (160 rpm) at 25ºC, with 2% glucose as the carbon source. Data was the means of three replicates. Bars at each point indicate ± SE.CC-BY · From the PDF
Figure 2 . Figure 2. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (C -strain 2F32) with diff erent carbon sources (♦ -2% glucose; ■ -2% sucrose). Cultures were incubated in a shaker (160 rpm) at pH 6.0, 25°C. Data was the means of three replicates. Bars at each point indicate ± SE.
Figure 2 . Figure 2. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (C -strain 2F32) with diff erent carbon sources (♦ -2% glucose; ■ -2% sucrose). Cultures were incubated in a shaker (160 rpm) at pH 6.0, 25°C. Data was the means of three replicates. Bars at each point indicate ± SE.CC-BY · From the PDF
Figure 3 . Figure 3. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (C -strain 2F32) at diff erent pH (♦ -pH 4.5; ■ -pH 6.0) with 2% sucrose as the carbon source. Cultures were incubated in a shaker (160 rpm) at 25ºC, pH 6.0. Data was the means of three replicates. Bars at each point indicate ± SE.
Figure 3 . Figure 3. IAA production and cell biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (C -strain 2F32) at diff erent pH (♦ -pH 4.5; ■ -pH 6.0) with 2% sucrose as the carbon source. Cultures were incubated in a shaker (160 rpm) at 25ºC, pH 6.0. Data was the means of three replicates. Bars at each point indicate ± SE.CC-BY · From the PDF
Figure 4 . Figure 4. IAA production and biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (Cstrain 2F32) at diff erent temperatures (♦-22°C; ■ -25°C; ▲-30°C). Cultures were incubated in a shaker (160 rpm) at pH 6.0, with glucose as the carbon source. Data was the means of three replicates. Bars at each point indicate ± SE.
Figure 4 . Figure 4. IAA production and biomass concentration of T. asahii (A -strain 3S44 and B -strain 4C06) and Rh. mucilaginosa (Cstrain 2F32) at diff erent temperatures (♦-22°C; ■ -25°C; ▲-30°C). Cultures were incubated in a shaker (160 rpm) at pH 6.0, with glucose as the carbon source. Data was the means of three replicates. Bars at each point indicate ± SE.CC-BY · From the PDF

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Metadata

Authors
Scarcella, A. S. D. A., Bizarria Junior, R., Bastos, R. G., & Magri, M. M. R.
Affiliations
  • Universidade de São Paulo, Brazil
  • Universidade Federal de São Carlos, Brazil
  • Universidade Federal de São Carlos (BR)
ORCID
Bastos, Reinaldo Gaspar
Venue
Brazilian Journal of Chemical Engineering
Publisher
FapUNIFESP (SciELO)
Published
2017-04
Volume
34
Issue
2
Pages
429-438
ISSN
0104-6632 (ISSN-L)
DOI
10.1590/0104-6632.20170342s20150541
OpenAlex
W2761881250
Cited by
21
References
1
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 2
FWCI
1.82
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Brazilian Journal of Chemical Engineering (publishedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Fundação de Amparo à Pesquisa do Estado de São Paulo (11/02322-0)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Growth Enhancement Techniques
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
Keywords
indole acetic acid, plant growth-promoting yeasts, Rhodotorula mucilaginosa, Trichosporon asahii, carbon source, glucose, sucrose, culture medium pH, temperature, phytohormone production, yeast growth, phylloplane yeasts

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Scarcella, A. S. D. A., Bizarria Junior, R., Bastos, R. G., & Magri, M. M. R. (2017). Temperature, pH and carbon source affect drastically indole acetic acid production of plant growth promoting yeasts. Brazilian Journal of Chemical Engineering. https://doi.org/10.1590/0104-6632.20170342s20150541