miR-23a-3p inhibits sepsis-induced kidney epithelial cell injury by suppressing Wnt/β-catenin signaling by targeting wnt5a
Open accessAbstract
The present study was designed to investigate the involvement of miR-23a-3p in the progression of sepsis-induced acute kidney injury (AKI). The expression levels of miR-23a-3p and wnt5a in sepsis-induced AKI patients and lipopolysaccharide (LPS)-treated HK-2 cells were detected by real-time PCR and western blotting. Then, the effects of miR-23a-3p overexpression on cell viability, apoptosis, and inflammatory cytokines secretion in LPS-stimulated HK-2 cells were investigated. Moreover, luciferase reporter assay was performed to confirm the regulatory relationship between miR-23a-3p and wnt5a. Whether miR-23a-3p regulated the activation of Wnt/β-catenin signaling was also explored. mR-23a-3p was lowly expressed in the serum of patients with sepsis-associated AKI and in LPS-treated HK-2 cells. In addition, the overexpression of miR-23a-3p restrained LPS-induced proliferation inhibition and promotion of apoptosis and cytokine production in HK-2 cells. Moreover, wnt5a was identified as a target of miR-23a-3p, which could be negatively regulated by miR-23a-3p. Overexpression of miR-23a-3p suppressed the activation of Wnt/β-catenin signaling in LPS-treated HK-2 cells, which was markedly reversed by wnt5a upregulation. Upregulation of miR-23a-3p may alleviate LPS-induced cell injury by targeting wnt5a and inactivating Wnt/β-catenin pathway, which may serve as a novel therapeutic target for sepsis-associated AKI.
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Overexpression of miR-23a-3p inhibits the lipopolysaccharide-induced HK-2 cell proliferation inhibition, promotes apoptosis, and reduces cytokine production.
“The overexpression of miR-23a-3p inhibits the lipopolysaccharide-induced HK-2 cell proliferation inhibition, promotes apoptosis, and reduces cytokine production.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [37]
The Results section reports that miR-23a-3p overexpression increased viability in LPS-treated HK-2 cells and reversed the rise in inflammatory cytokines. But it significantly attenuated apoptosis rather than promoting it. Page numbers are not provided in the supplied passages. (LLM, conf=0.92)
Experimental — accuracy not yet measuredmiR-23a-3p is downregulated in the serum and lipopolysaccharide-treated HK-2 cells of patients with sepsis-associated acute kidney injury.
“Moreover, miR-23a-3p is downregulated in the serum and lipopolysaccharide-treated HK-2 cells of patients with sepsis-associated acute kidney injury.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [37]
miR-23a can alleviate lipopolysaccharide-induced cell damage by targeting wnt5a to induce Wnt/β-catenin pathway deactivation.
“miR-23a can alleviate the lipopolysaccharide-induced cell damage by targeting wnt5a to induce the Wnt/β-catenin pathway deactivation [37]”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [37]
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- Authors
- Ye, J., Feng, H., & Peng, Z.
- Affiliations
- Zhongnan Hospital of Wuhan University (CN)
- Hubei Polytechnic University (CN)
- Venue
- Brazilian Journal of Medical and Biological Research
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2022
- Volume
- 55
- Pages
- e11571-e11571
- ISSN
- 0100-879X (ISSN-L)
- 1414-431X
- PMC
- PMC8905671
- OpenAlex
- W4214865351
- Cited by
- 17
- References
- 32
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 5 · 2023: 3 · 2022: 2
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Brazilian Journal of Medical and Biological Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Nephrology › Acute Kidney Injury Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Epidemiology › Sepsis Diagnosis and Treatment
- Keywords
- miR-23a-3p, Wnt5a, sepsis-associated acute kidney injury, Wnt/β-catenin signaling, renal epithelial cells, HK-2 cells, lipopolysaccharide, apoptosis, inflammatory cytokines, microRNA target regulation, therapeutic target
- MeSH terms
- Wnt-5a Protein, Epithelial Cells, Humans, Kidney, Apoptosis, Sepsis, MicroRNAs, beta Catenin, Wnt Signaling Pathway
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