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Effect of salbutamol on innervated and denervated rat soleus muscle
Open accessAbstract
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a beta2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for myosin ATPase, pH 9.4. Cross-sectional area and percent of muscle fibers were analyzed morphometrically by computerized image analysis. Treatment with salbutamol induced hypertrophy of all fiber types and a higher percentage of type II fibers (21%) in the healthy rat soleus muscle. Denervation caused marked atrophy of all fibers and conversion from type I to type II muscle fibers. Denervated muscles treated with salbutamol showed a significantly larger cross-sectional area of type I muscle fibers, 28.2% compared to the denervated untreated muscle. Moreover, the number of type I fibers was increased. These results indicate that administration of salbutamol is able to induce changes in cross-sectional area and fiber type distribution in the early phase of treatment. Since denervation-induced atrophy and conversion from type I to type II fibers were improved by salbutamol treatment we propose that salbutamol, like other beta2 adrenoceptor agonists, may have a therapeutic potential in improving the condition of skeletal muscle after denervation.
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β2-adrenergic agonists such as salbutamol, clenbuterol, and ractopamine promote muscle hypertrophy in animal models.
“β2-adrenergic agonist such as salbutamol [40], clenbuterol [41], and ractopamine [42] promote muscle hypertrophy in animal models.”
From Effects of Skeletal Muscle Hypertrophy on Fat Mass and Glucose Homeostasis in Humans and Animals: A Narrative Review with Systematic Literature Search (2025) · cites this paper as [40]
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Role of β-Adrenoceptor Signaling in Skeletal Muscle: Implications for Muscle Wasting and Disease
Lynch, G. S., & Ryall, J. G. (2008) · Physiological Reviews · doi:10.1152/physrev.00028.2007 · Cited by 410
Havers, T., Held, S., Schönfelder, M., Geisler, S., & Wackerhage, H. (2025) · Sports Medicine · doi:10.1007/s40279-025-02263-w · Cited by 19
“4. β2-adrenergic agonist such as salbutamol [40], clen-buterol [41], and ractopamine [42] promote muscle hypertrophy in animal models.”
Metadata
- Authors
- Šoic-Vranic, T., Bobinac, D., Bajek, S., Jerkovic, R., Malnar-Dragojevic, D., & Nikolic, M.
- Affiliations
- University of Rijeka, Croatia
- University of Rijeka (HR)
- ORCID
- Venue
- Brazilian Journal of Medical and Biological Research
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2005-12
- Volume
- 38
- Issue
- 12
- Pages
- 1799-1805
- ISSN
- 0100-879X (ISSN-L)
- OpenAlex
- W2120559804
- Cited by
- 19
- References
- 21
- Citations by year
- 2025: 2 · 2024: 1 · 2020: 2 · 2019: 1 · 2013: 4 · 2012: 1
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Brazilian Journal of Medical and Biological Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
- Keywords
- salbutamol, beta2-adrenergic agonists, soleus muscle, denervation, muscle atrophy, muscle hypertrophy, type I muscle fibers, fast-twitch muscle, cross-sectional area
- MeSH terms
- Adrenergic beta-Agonists, Albuterol, Animals, Male, Muscle Denervation, Muscular Atrophy, Rats, Wistar, Muscle, Skeletal, Muscle Fibers, Skeletal, Rats
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