In vitro Antagonistic Growth Effects of Lactobacillus fermentum and Lactobacillus salivarius and Their Fermentative Broth on Periodontal Pathogens
Open accessAbstract
As lactobacilli possess an antagonistic growth property, these bacteria may be beneficial as bioprotective agents for infection control. However, whether the antagonistic growth effects are attributed to the lactobacilli themselves or their fermentative broth remains unclear. The antagonistic growth effects of Lactobacillus salivarius and Lactobacillus fermentum as well as their fermentative broth were thus tested using both disc agar diffusion test and broth dilution method, and their effects on periodontal pathogens, including Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalis in vitro at different concentrations and for different time periods were also compared. Both Lactobacillus salivarius and Lactobacillus fermentum and their concentrated fermentative broth were shown to inhibit significantly the growth of Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalis, although different inhibitory effects were observed for different pathogens. The higher the counts of lactobacilli and the higher the folds of concentrated fermentative broth, the stronger the inhibitory effects are observed. The inhibitory effect is demonstrated to be dose-dependent. Moreover, for the lactobacilli themselves, Lactobacillus fermentum showed stronger inhibitory effects than Lactobacillus salivarius. However, the fermentative broth of Lactobacillus fermentum showed weaker inhibitory effects than that of Lactobacillus salivarius. These data suggested that lactobacilli and their fermentative broth exhibit antagonistic growth activity, and consumption of probiotics or their broth containing lactobacilli may benefit oral health.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Postbiotics of Lactobacillus fermentum, Lactobacillus salivarius, and Lactococcus lactis can suppress the growth of opportunistic or pathogenic bacteria such as Streptococcus sanguinis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola.
“The postbiotics of Lactobacillus fermentum, Lactobacillus salivarius, and Lactococcus lactis can suppress the growth of opportunistic or pathogenic bacteria such as Streptococcus sanguinis, Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola [11,12].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [11]
The Results (Broth dilution method; page not provided) report that concentrated fermentative broths of L. fermentum and L. salivarius inhibited S. mutans, S. sanguis, and P. gingivalis in vitro. The paper does not support the attributed effects of Lactococcus lactis or effects against F. nucleatum, T. forsythia, or T. denticola; it names S. sanguis rather than S. sanguinis. (LLM, conf=0.90)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Probiotics, Prebiotics, and Synbiotics
de Vrese, M., & Schrezenmeir, J. (2008) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2008_097 · Cited by 1,034 · see citing works
Näse, L., Hatakka, K., Savilahti, E., Saxelin, M., Pönkä, A., Poussa, T. A., Korpela, R., & Meurman, J. H. (2001) · Caries Research · doi:10.1159/000047484 · Cited by 581 · see citing works
Ouhara, K., Komatsuzawa, H., Yamada, S., Shiba, H., Fujiwara, T., Ohara, M., Sayama, K., Hashimoto, K., Kurihara, H., & Sugai, M. (2005) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dki103 · Cited by 427 · see citing works
Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors
Ahola, A. J., Yli‐Knuuttila, H., Suomalainen, T., Poussa, T. A., Ahlström, A., Meurman, J. H., & Korpela, R. (2002) · Archives of Oral Biology · doi:10.1016/s0003-9969(02)00112-7 · Cited by 387 · see citing works
Probiotics: contributions to oral health
Meurman, J. H., & Stamatova, I. (2007) · Oral Diseases · doi:10.1111/j.1601-0825.2007.01386.x · Cited by 337 · see citing works
Snydman, D. R. (2008) · Clinical Infectious Diseases · doi:10.1086/523331 · Cited by 336 · see citing works
Kõll-Klais, P., Mändar, R., Leibur, E., Marcotte, H., Hammarström, L. L. G., & Mikelsaar, M. (2005) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2005.00239.x · Cited by 328 · see citing works
Probiotics, Prebiotics and Synbiotics
Hawrelak, J. (2007) · eCite Digital Repository (University of Tasmania) · Cited by 320 · see citing works
Gupta, V, & Garg, R. (2009) · Indian Journal of Medical Microbiology · doi:10.4103/0255-0857.53201 · Cited by 283 · see citing works
The Role of Local Drug Delivery in the Management of Periodontal Diseases: A Comprehensive Review
Greenstein, G., & Polson, A. M. (1998) · Journal of Periodontology · doi:10.1902/jop.1998.69.5.507 · Cited by 262 · see citing works
Bacteriotherapy and probiotics’ role on oral health
Çağlar, E., Kargül, B., & Tanboğa, İ. H. (2005) · Oral Diseases · doi:10.1111/j.1601-0825.2005.01109.x · Cited by 249 · see citing works
Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci
Nikawa, H., Makihira, S., Fukushima, H., NISHIMURA, H., Ozaki, Y., Ishida, K., Darmawan, S., Hamada, T., Hara, K., Matsumoto, A., et al. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.006 · Cited by 237 · see citing works
Antimicrobial potential of four Lactobacillus strains isolated from breast milk
Olivares, M. M., Díaz-Ropero, M. P., Martı́n, R., Rodríguez, J. M., & Xaus, J. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.02981.x · Cited by 226 · see citing works
Sutyak, K. E., Wirawan, R. E., Aroutcheva, A. A., & Chikindas, M. L. (2007) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2007.03626.x · Cited by 207 · see citing works
Characterization of oral lactobacilli as potential probiotics for oral health
Kõll-Klais, P., Mändar, R., Marcotte, H., Leibur, E., Mikelsaar, M., & Hammarström, L. L. G. (2008) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2007.00402.x · Cited by 172 · see citing works
Antimicrobial resistance in the subgingival microflora in patients with adult periodontitis
Winkelhoff, A. J. V., Gonzales, D. H., Winkel, E. G., Dellemijn‐Kippuw, N., Vandenbroucke‐Grauls, C. M. J. E., & Sanz, M. (2000) · Journal Of Clinical Periodontology · doi:10.1034/j.1600-051x.2000.027002079.x · Cited by 171 · see citing works
Çağlar, E., Kuşcu, Ö. Ö., Kuvvetli, S. S., Çildir, Ş. K., Sandallı, N., & Twetman, S. H. A. (2008) · Acta Odontologica Scandinavica · doi:10.1080/00016350802089467 · Cited by 155 · see citing works
Antimicrobial activity of silver nitrate against periodontal pathogens
Spacciapoli, P., Buxton, D., Rothstein, D., et al. (2001) · Journal of Periodontal Research · doi:10.1034/j.1600-0765.2001.360207.x · Cited by 129 · see citing works
Antimicrobial activity of nisin against Oenococcus oeni and other wine bacteria
Rojo‐Bezares, B., Sáenz, Y., Zarazaga, M., Torres, C., & Ruiz‐Larrea, F. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.12.020 · Cited by 116 · see citing works
McGroarty, J. A., Tomeczek, L., Pond, D. G., et al. (1992) · The Journal of Infectious Diseases · doi:10.1093/infdis/165.6.1142 · Cited by 114 · see citing works
Bifidobacteria and lactobacilli in human health.
Orrhage, K., & Ce, N. (2000) · PubMed · Cited by 109 · see citing works
Millette, M., Dupont, C., Archambault, D., & Lacroix, M. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.03044.x · Cited by 76 · see citing works
Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients
Thiha, K., Takeuchi, Y., Umeda, M., Huang, Y., Ohnishi, M., & Ishikawa, I. (2007) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2007.00354.x · Cited by 73 · see citing works
Distribution of SalivaryLactobacillusandBifidobacteriumSpecies in Periodontal Health and Disease
Hojo, K., Mizoguchi, C., Taketomo, N., Ohshima, T., Gomi, K., Arai, T., & Maeda, N. (2007) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.60420 · Cited by 69 · see citing works
Isolation of lactobacilli from sow milk and evaluation of their probiotic potential
Martı́n, R., Delgado, S., Maldonado‐Barragán, A., et al. (2009) · Journal of Dairy Research · doi:10.1017/s0022029909990124 · Cited by 62 · see citing works
Antimicrobial Properties of Milkfat Globule Membrane Fractions
Clare, D. A., Zheng, Z., Hassan, H. M., Swaisgood, H. E., & Catignani, G. L. (2008) · Journal of Food Protection · doi:10.4315/0362-028x-71.1.126 · Cited by 55 · see citing works
Dover, S. E., Aroutcheva, A. A., Faro, S., & Chikindas, M. L. (2007) · Infectious Diseases in Obstetrics and Gynecology · doi:10.1155/2007/78248 · Cited by 48 · see citing works
Ruíz, F. O., Gerbaldo, G. A., Asurmendi, P., et al. (2009) · Current Microbiology · doi:10.1007/s00284-009-9465-0 · Cited by 40 · see citing works
Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci*1
NIKAWA, H. (2004) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(04)00155-2 · Cited by 36 · see citing works
Wasserman, B. H., & Hirschfeld, L. S. (1988) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1988.tb01552.x · Cited by 33 · see citing works
Vančíková, Z., Lodinová‐Žádníková, R., Radl, J., et al. (2003) · Folia Microbiologica · doi:10.1007/bf02930970 · Cited by 21 · see citing works
PHARMACOLOGIC MANAGEMENT OF PERIODONTAL DISEASES USING SYSTEMICALLY ADMINISTERED AGENTS
Mariotti, A. J., & Monroe, P. J. (1998) · Dental Clinics of North America · doi:10.1016/s0011-8532(22)00589-4 · Cited by 20 · see citing works
Winkelhoff, A. J. V., Herrera, D., Winkel, E. G., Dellemijn‐Kippuw, N., Vandenbroucke‐Grauls, C. M. J. E., & Sanz, M. (1999) · PubMed · Cited by 19 · see citing works
Periodontal Bacterial DNA Suppresses the Immune Response to Mutans Streptococcal Glucosyltransferase
Taubman, M. A., Han, X., LaRosa, K. B., Socransky, S. S., & Smith, D. J. (2007) · Infection and Immunity · doi:10.1128/iai.00623-07 · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Karaca, B., Haliscelik, O., Gursoy, M., Kiran, F., Loimaranta, V., Söderling, E., & Gursoy, U. K. (2022) · Microorganisms · doi:10.3390/microorganisms10112200 · Cited by 26 · see citing works
- Authors
- Chen, L.-J., Tsai, H.-T., Chen, W.-J., Hsieh, C.-Y., Wang, P.-C., Chen, C.-S., Wang, L., & Yang, C.-C.
- Affiliations
- Pojen General Hospital (TW)
- Chung Shan Medical University (TW)
- Chaoyang University of Technology (TW)
- Chung Shan Medical University Hospital (TW)
- Venue
- Brazilian Journal of Microbiology
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2012-12
- Volume
- 43
- Issue
- 4
- Pages
- 1376-1384
- ISSN
- 1517-8382 (ISSN-L)
- PMC
- PMC3769017
- OpenAlex
- W2013390797
- Cited by
- 29
- References
- 34
- Citations by year
- 2024: 1 · 2023: 6 · 2022: 2 · 2021: 8 · 2020: 5 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 1
- FWCI
- 1.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Brazilian Journal of Microbiology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Chung Shan Medical University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus fermentum, Lactobacillus salivarius, periodontal pathogens, Streptococcus mutans, Streptococcus sanguinis, Porphyromonas gingivalis, probiotics, antibacterial activity, oral health, dose-dependent inhibition
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
