The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Identification of Mosquito Bloodmeals Using Polymerase Chain Reaction (PCR) With Order-Specific Primers
Abstract
A polymerase chain reaction (PCR) protocol was developed to identify host bloodmeals from mosquitoes. Primers for the cytochrome b gene were designed to distinguish between mammalian and avian bloodmeals and further differentiate among four avian orders: passeriformes, falconiformes, columbiformes, and galliformes. The assay was validated by testing tissues from 18 species of passeriformes, three species of falconiformes, three species of columbiformes, and two species of galliformes. American crows were distinguished from other passeriformes by restriction enzyme digestion. Host bloodmeals from engorged mosquitoes collected in New York State were identified to avian order level. PCR was able to detect the mosquito bloodmeal for up to 3 d after feeding on a quail. Significantly, these studies use order-specific primers in a single PCR test to identify mosquito bloodmeals.
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Cites
Lanciotti, R. S., Roehrig, J. T., Deubel, V., Smith, J., Parker, M., Steele, K., Crise, B., Volpe, K. E., Crabtree, M. B., Scherret, J. H., et al. (1999) · Science · doi:10.1126/science.286.5448.2333 · Cited by 1,593
West Nile Virus Infection in Birds and Mosquitoes, New York State, 2000
Bernard, K. A., Maffei, J. G., Jones, S. A., Kauffman, E. B., Ebel, G. D., Dupuis, A. P., Ngo, K. A., Nicholas, D. C., Young, D. M., Shi, P.-Y., et al. (2001) · Emerging Infectious Diseases · doi:10.3201/eid0704.017415 · Cited by 172
Self-citation
Cited by
West Nile Virus Epidemics in North America Are Driven by Shifts in Mosquito Feeding Behavior
Kilpatrick, A. M., Kramer, L. D., Jones, M. J., Marra, P. P., & Daszak, P. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040082 · Cited by 608
Self-citationHost heterogeneity dominates West Nile virus transmission
Marm Kilpatrick, A., Daszak, P., Jones, M. J., Marra, P. P., & Kramer, L. D. (2006) · Proceedings of the Royal Society B: Biological Sciences · doi:10.1098/rspb.2006.3575 · Cited by 533
Self-citationEpidemiology of West Nile Virus in Connecticut: A Five-Year Analysis of Mosquito Data 1999–2003
Andreadis, T. G., Anderson, J. F., Vossbrinck, C. R., & Main, A. J. (2004) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2004.4.360 · Cited by 262
MOLAEI, G., OLIVER, J., ANDREADIS, T. G., ARMSTRONG, P. M., & HOWARD, J. J. (2006) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2006.75.1140 · Cited by 84
Metadata
- Authors
- Ngo, K. A., & Kramer, L. D.
- Venue
- Journal of Medical Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-03-01
- Volume
- 40
- Issue
- 2
- Pages
- 215-222
- ISSN
- 0022-2585 (ISSN-L)
- OpenAlex
- W2120172535
- Cited by
- 229
- References
- 2
- FWCI
- 9.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ngo, K. A., & Kramer, L. D. (2003). Identification of Mosquito Bloodmeals Using Polymerase Chain Reaction (PCR) With Order-Specific Primers. Journal of Medical Entomology. https://doi.org/10.1603/0022-2585-40.2.215
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