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Cited by 84Mosquito-borne diseases and controlDiptera species taxonomy and behaviorVector-borne infectious diseases

Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of Eastern Equine Encephalitis virus in New York

MOLAEI, GOUDARZ, OLIVER, JOANNE, ANDREADIS, THEODORE G., ARMSTRONG, PHILIP M., HOWARD, JOHN J.·2006·The American Journal of Tropical Medicine and Hygiene
DOI 10.4269/ajtmh.2006.75.1140
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Abstract

Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.

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Authors
MOLAEI, G., OLIVER, J., ANDREADIS, T. G., ARMSTRONG, P. M., & HOWARD, J. J.
Affiliations
New York State Department of Health (US)
ORCID
Venue
The American Journal of Tropical Medicine and Hygiene
Publisher
American Society of Tropical Medicine and Hygiene
Published
2006-12
Volume
75
Issue
6
Pages
1140-1147
ISSN
0002-9637 (ISSN-L)
DOI
10.4269/ajtmh.2006.75.1140
OpenAlex
W2130281693
Cited by
84
References
37
Citations by year
2026: 3 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 4 · 2017: 6 · 2016: 3 · 2015: 7 · 2014: 3 · 2013: 8 · 2012: 7
FWCI
3.33
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Tropical Medicine and Hygiene (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. Public Health Service (U50/CCU6806-01-1)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Diptera species taxonomy and behavior
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
Keywords
eastern equine encephalitis virus, Culiseta melanura, Culiseta morsitans, avian hosts, wood thrush, Passeriformes, mammalian hosts, white-tailed deer, arbovirus transmission, mosquito-borne arboviruses, epizootiology, New York, cytochrome b
MeSH terms
Animals, Blood, Cats, Deer, Diptera, Disease Vectors, DNA, Viral, Encephalitis Virus, Eastern Equine, Horses, Mammals, Culicidae, New York, Vertebrates, Polymerase Chain Reaction, Songbirds

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