The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Directed Differentiation of Dopaminergic Neuronal Subtypes from Human Embryonic Stem Cells
Open accessAbstract
How dopamine (DA) neuronal subtypes are specified remains unknown. In this study we show a robust generation of functional DA neurons from human embryonic stem cells (hESCs) through a specific sequence of application of fibroblast growth factor 8 (FGF8) and sonic hedgehog (SHH). Treatment of hESC-derived Sox1+ neuroepithelial cells with FGF8 and SHH resulted in production of tyrosine hydroxylase (TH)-positive neurons that were mostly bipolar cells, coexpression with gamma-aminobutyric acid, and lack of midbrain marker engrailed 1 (En1) expression. However, FGF8 treatment of precursor cells before Sox1 expression led to the generation of a similar proportion of TH+ neurons characteristic of midbrain projection DA neurons with large cell bodies and complex processes and coexpression of En1. This suggests that one mechanism of generating neuronal subtypes is temporal availability of morphogens to a specific group of precursors. The in vitro-generated DA neurons were electrophysiologically active and released DA in an activity-dependent manner. They may thus provide a renewable source of functional human DA neurons for drug screening and development of sustainable therapeutics for disorders affecting the DA system.
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Hamill, O. P., Marty, A., Neher, E., Sakmann, B., & Sigworth, F. J. (1981) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/bf00656997 · Cited by 18,436
Neuronal specification in the spinal cord: inductive signals and transcriptional codes
Jessell, T. M. (2000) · Nature Reviews Genetics · doi:10.1038/35049541 · Cited by 2,089
[6] Correction for liquid junction potentials in patch clamp experiments
Neher, E. (1992) · Methods in Enzymology · doi:10.1016/0076-6879(92)07008-c · Cited by 1,132
Cited by
Hu, B.-Y., Weick, J. P., Yu, J., Ma, L.-X., Zhang, X.-Q., Thomson, J. A., & Zhang, S.-C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910012107 · Cited by 1,013
Self-citationEngineering Stem Cell Organoids
Yin, X., Mead, B. E., Safaee, H., Langer, R., Karp, J. M., & Levy, O. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2015.12.005 · Cited by 866
Metabolic oxidation regulates embryonic stem cell differentiation
Yanes, O., Clark, J., Wong, D. M., Patti, G. J., Sánchez-Ruiz, A., Benton, H. P., Trauger, S. A., Desponts, C., Ding, S., & Siuzdak, G. (2010) · Nature Chemical Biology · doi:10.1038/nchembio.364 · Cited by 506
Pax6 is a human neuroectoderm cell fate determinant
Zhang, X., Huang, C. T., Chen, J., Pankratz, M. T., Xi, J., Li, J., Yang, Y., LaVaute, T. M., Li, X.-J., Ayala, M., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.04.017 · Cited by 480
Self-citationPluripotent stem cells in regenerative medicine: challenges and recent progress
Tabar, V., & Studer, L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3563 · Cited by 458
Metadata
- Authors
- Yan, Y., Yang, D., Zarnowska, E. D., Du, Z., Werbel, B., Valliere, C., Pearce, R. A., Thomson, J. A., & Zhang, S.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-06
- Volume
- 23
- Issue
- 6
- Pages
- 781-790
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2083939751
- Cited by
- 488
- References
- 3
- FWCI
- 13.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yan, Y., Yang, D., Zarnowska, E. D., Du, Z., Werbel, B., Valliere, C., Pearce, R. A., Thomson, J. A., & Zhang, S. (2005). Directed Differentiation of Dopaminergic Neuronal Subtypes from Human Embryonic Stem Cells. STEM CELLS. https://doi.org/10.1634/stemcells.2004-0365
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