Engineering Stem Cell Organoids
Open accessAbstract
Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.
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Matrigel is likely the most widely-used material used for organoid generation, composed of collagen IV, laminin, and entactin derived from the Engelbreth− Holm−Swarm (EHS) mouse sarcoma which thermally crosslink to form a hydrogel.
“Matrigel is likely the most widely-used material used for organoid generation [17], composed of collagen IV, laminin, and entactin derived from the Engelbreth− Holm−Swarm (EHS) mouse sarcoma which thermally crosslink to form a hydrogel.”
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Solé, R., Ollé-Vila, A., Vidiella, B., Duran-Nebreda, S., & Conde-Pueyo, N. (2018) · Current Opinion in Systems Biology · doi:10.1016/j.coisb.2017.11.007 · Cited by 19 · see citing works
Quílez, C., Jeon, E. Y., Pappalardo, A., Pathak, P., & Abaci, H. E. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202400405 · Cited by 22 · see citing works
- Authors
- Yin, X., Mead, B. E., Safaee, H., Langer, R., Karp, J. M., & Levy, O.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Karp, Jeffrey M.
- Levy, Oren
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 18
- Issue
- 1
- Pages
- 25-38
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC4728053
- OpenAlex
- W2228561711
- Cited by
- 866
- References
- 17
- Citations by year
- 2026: 55 · 2025: 76 · 2024: 68 · 2023: 66 · 2022: 90 · 2021: 131 · 2020: 104 · 2019: 91 · 2018: 88 · 2017: 67 · 2016: 30
- FWCI
- 6.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-nd)
- Funders
- National Institutes of Health (HL095722, DE013023)
- National Science Foundation
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- organoid engineering, cell-cell interactions, cell-matrix interactions, tissue models, regenerative therapy
- MeSH terms
- Animals, Brain, Cell Communication, Humans, Intestinal Mucosa, Organoids, Regeneration, Stem Cells, Cell Culture Techniques, Tissue Engineering, Intercellular Signaling Peptides and Proteins, Organogenesis, Microfluidics
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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