The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage
Open accessAbstract
Understanding neuroectoderm formation and subsequent diversification to functional neural subtypes remains elusive. We show here that human embryonic stem cells (hESCs) differentiate to primitive neuroectoderm after 8–10 days. At this stage, cells uniformly exhibit columnar morphology and express neural markers, including anterior but not posterior homeodomain proteins. The anterior identity of these cells develops regardless of morphogens present during initial neuroectoderm specification. This anterior phenotype can be maintained or transformed to a caudal fate with specific morphogens over the next week, when cells become definitive neuroepithelia, marked by neural tube-like structures with distinct adhesion molecule expression, Sox1 expression, and a resistance to additional patterning signals. Thus, primitive neuroepithelia represents the earliest neural cells that possess the potential to differentiate to regionally specific neural progenitors. This finding offers insights into early human brain development and lays a foundation for generating neural cells with correct positional and transmitter profiles. Disclosure of potential conflicts of interest is found at the end of this article.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Multipotent cell lineages in early mouse development depend on SOX2 function
Avilion, A. A., Nicolis, S. K., Pevny, L. H., Perez, L., Vivian, N., & Lovell-Badge, R. (2003) · Genes & Development · doi:10.1101/gad.224503 · Cited by 2,369
Developing human : clinically oriented embryology
Moore, K. L., Persaud, T. V. N., & Torchia, M. G. (1973) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 2,299
Control of Developmental Regulators by Polycomb in Human Embryonic Stem Cells
Lee, T. I., Jenner, R. G., Boyer, L. A., Guenther, M. G., Levine, S. S., Kumar, R. M., Chevalier, B., Johnstone, S. E., Cole, M. F., Isono, K.-I., et al. (2006) · Cell · doi:10.1016/j.cell.2006.02.043 · Cited by 2,268
In vitro differentiation of transplantable neural precursors from human embryonic stem cells
Zhang, S.-C., Wernig, M., Duncan, I. D., Brüstle, O., & Thomson, J. A. (2001) · Nature Biotechnology · doi:10.1038/nbt1201-1129 · Cited by 1,912
Self-citationDirected Differentiation of Embryonic Stem Cells into Motor Neurons
Wichterle, H., Lieberam, I., Porter, J. A., & Jessell, T. M. (2002) · Cell · doi:10.1016/s0092-8674(02)00835-8 · Cited by 1,728
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
The development of neural stem cells
Temple, S. (2001) · Nature · doi:10.1038/35102174 · Cited by 1,491
Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell–Derived Inducing Activity
Kawasaki, H., Mizuseki, K., Nishikawa, S., Kaneko, S., Kuwana, Y., Nakanishi, S., Nishikawa, S.-I., & Sasai, Y. (2000) · Neuron · doi:10.1016/s0896-6273(00)00083-0 · Cited by 1,277
Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells
Lee, S.-H., Lumelsky, N., Studer, L., Auerbach, J. M., & McKay, R. D. (2000) · Nature Biotechnology · doi:10.1038/76536 · Cited by 1,257
Pax-6, a murine paired box gene, is expressed in the developing CNS
Walther, C., & Gruss, P. (1991) · Development · doi:10.1242/dev.113.4.1435 · Cited by 1,255
Cited by
Hu, B.-Y., Weick, J. P., Yu, J., Ma, L.-X., Zhang, X.-Q., Thomson, J. A., & Zhang, S.-C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910012107 · Cited by 1,013
Self-citationHuman ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage
Elkabetz, Y., Panagiotakos, G., Al Shamy, G., Socci, N. D., Tabar, V., & Studer, L. (2008) · Genes & Development · doi:10.1101/gad.1616208 · Cited by 677
Kirkeby, A., Grealish, S., Wolf, D. A., Nelander, J., Wood, J., Lundblad, M., Lindvall, O., & Parmar, M. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.04.009 · Cited by 662
Modeling early retinal development with human embryonic and induced pluripotent stem cells
Meyer, J. S., Shearer, R. L., Capowski, E. E., Wright, L. S., Wallace, K. A., McMillan, E. L., Zhang, S.-C., & Gamm, D. M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0905245106 · Cited by 601
Self-citationUsing iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome
Paşca, S. P., Portmann, T., Voineagu, I., Yazawa, M., Shcheglovitov, A., Paşca, A. M., Cord, B., Palmer, T. D., Chikahisa, S., Nishino, S., et al. (2011) · Nature Medicine · doi:10.1038/nm.2576 · Cited by 585
Metadata
- Authors
- Pankratz, M. T., Li, X.-J., LaVaute, T. M., Lyons, E. A., Chen, X., & Zhang, S.-C.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-03-01
- Volume
- 25
- Issue
- 6
- Pages
- 1511-1520
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2074498796
- Cited by
- 317
- References
- 48
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage. https://app.cochat.ai/papers/10.1634/stemcells.2006-0707
- The paper
- Pankratz, M. T., Li, X.-J., LaVaute, T. M., Lyons, E. A., Chen, X., & Zhang, S.-C. (2007). Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage. Stem Cells. https://doi.org/10.1634/stemcells.2006-0707
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
