Human Embryonic Stem Cells Have Enhanced Repair of Multiple Forms of DNA Damage
Open accessAbstract
Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells. Disclosure of potential conflicts of interest is found at the end of this article.
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Several protective mechanisms have been detected in different stem cell types for protecting against reactive oxygen species and ultraviolet radiation-induced DNA damage.
“Regarding the importance of stem cells, several protective mechanisms have been detected in different stem cell types for protecting against ROS and UV-induced DNA damage [127, 128].”
From Ultraviolet Radiation‐Induced Skin Aging: The Role of DNA Damage and Oxidative Stress in Epidermal Stem Cell Damage Mediated Skin Aging (2016) · cites this paper as [128]
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- Authors
- Maynard, S., Swistowska, A. M., Lee, J. W., Liu, Y., Liu, S.-T., Da Cruz, A. B., Rao, M., de Souza-Pinto, N. C., Zeng, X., & Bohr, V. A.
- Affiliations
- National Institutes of Health (US)
- Buck Institute for Research on Aging (US)
- United States Patent and Trademark Office (US)
- Invitrogen Corporation, Stem Cell and Regenerative Medicine, Carlsbad, California, USA
- Buck Institute for Age Research, Novato, California, USA
- Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, Maryland, USA
- Corresponding author
- Bohr, Vilhelm A.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-06-19
- Volume
- 26
- Issue
- 9
- Pages
- 2266-2274
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- PMC
- PMC2574957
- OpenAlex
- W2049217916
- Cited by
- 220
- References
- 52
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 9 · 2023: 7 · 2022: 7 · 2021: 7 · 2020: 16 · 2019: 9 · 2018: 9 · 2017: 15 · 2016: 17 · 2015: 10 · 2014: 15 · 2013: 15 · 2012: 30
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Stem Cells (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (Z01AG000726)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- human embryonic stem cells, DNA repair, genome stability, genomic surveillance, DNA damage, DNA repair gene expression, embryoid bodies, fibroblasts, HeLa cells, oxidative DNA damage, ionizing radiation, psoralen, comet assay
- MeSH terms
- Cell Differentiation, DNA Damage, DNA Repair, Fibroblasts, Ficusin, HeLa Cells, Humans, Hydrogen Peroxide, Radiation, Ionizing, Ultraviolet Rays, Oligonucleotide Array Sequence Analysis, Comet Assay, Embryonic Stem Cells
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