Cited by 677Pluripotent Stem Cells ResearchCRISPR and Genetic EngineeringMesenchymal stem cell research

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Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette

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Sommer, Cesar A., Stadtfeld, Matthias, Murphy, George J., Hochedlinger, Konrad, Kotton, Darrell N., Mostoslavsky, Gustavo·2009·Stem Cells

DOI 10.1634/stemcells.2008-1075PMC PMC4848035

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Abstract

Induced pluripotent stem (iPS) cells can be generated using retroviral vectors expressing Oct4, Klf4, Sox2, and cMyc. Most prior studies have required multiple retroviral vectors for reprogramming, resulting in high numbers of genomic integrations in iPS cells and limiting their use for therapeutic applications. Here we describe the use of a single lentiviral vector expressing a “stem cell cassette” composed of the four transcription factors and a combination of 2A peptide and internal ribosome entry site technology, generating iPS cells from postnatal fibroblasts. iPS cells generated in this manner display embryonic stem cell-like morphology, express stem cell markers, and exhibit in vivo pluripotency, as evidenced by their ability to differentiate in teratoma assays and their robust contribution to mouse chimeras. Combining all factors into a single transcript achieves the most efficient reprogramming system to date and allows derivation of iPS cells with a single viral integration. The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes.

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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,438
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Mikkelsen, T. S., Ku, M., Jaffe, D. B., Issac, B., Lieberman, E., Giannoukos, G., Alvarez, P., Brockman, W., Kim, T.-K., Koche, R. P., et al. (2007) · Nature · doi:10.1038/nature06008 · Cited by 4,129
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
Niwa, H., Miyazaki, J., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,904
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Wernig, M., Meissner, A., Foreman, R. K., Brambrink, T., Ku, M., Hochedlinger, K., Bernstein, B., & Jaenisch, R. (2007) · Nature · doi:10.1038/nature05944 · Cited by 2,732
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Meissner, A., Mikkelsen, T. S., Gu, H., Wernig, M., Hanna, J., Sivachenko, A., Zhang, X., Bernstein, B. E., Nusbaum, C., Jaffe, D. B., et al. (2008) · Nature · doi:10.1038/nature07107 · Cited by 2,536
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
Okita, K., Nakagawa, M., Hong, H., Ichisaka, T., & Yamanaka, S. (2008) · Science · doi:10.1126/science.1164270 · Cited by 2,056
Differentiation of Embryonic Stem Cells to Clinically Relevant Populations: Lessons from Embryonic Development
Murry, C. E., & Keller, G. M. (2008) · Cell · doi:10.1016/j.cell.2008.02.008 · Cited by 1,760

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Authors: Sommer, C. A., Stadtfeld, M., Murphy, G. J., Hochedlinger, K., Kotton, D. N., & Mostoslavsky, G.
Affiliations: Boston University (US)
Massachusetts General Hospital (US)
Harvard University (US)
Corresponding author: Mostoslavsky, Gustavo
ORCID: Sommer, Cesar A.
Stadtfeld, Matthias
Murphy, George J.
Hochedlinger, Konrad
Kotton, Darrell N.
Mostoslavsky, Gustavo
Venue: Stem Cells
Publisher: Oxford University Press (OUP)
Published: 2009-03-01
Volume: 27
Issue: 3
Pages: 543-549
ISSN: 1066-5099 (ISSN-L)
1549-4918
DOI: 10.1634/stemcells.2008-1075
PMC: PMC4848035
OpenAlex: W2038815755
Cited by: 676
References: 24
Citations by year: 2026: 6 · 2025: 13 · 2024: 16 · 2023: 15 · 2022: 18 · 2021: 23 · 2020: 30 · 2019: 35 · 2018: 42 · 2017: 41 · 2016: 41 · 2015: 49 · 2014: 61 · 2013: 65 · 2012: 66
FWCI: 21.14
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations: Stem Cells (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: V Foundation for Cancer Research
National Institutes of Health (1dp2od003266-01)
Harvard Stem Cell Institute
National Heart, Lung, and Blood Institute (P01HL047049)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (R21HD060308)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Keywords: induced pluripotent stem cells, lentiviral vectors, Oct4, KLF4, SOX2, c-Myc, 2A peptide, internal ribosome entry site, fibroblast reprogramming, chromosomal integration, teratoma assay, mouse chimeras, pluripotency
MeSH terms: Kruppel-Like Factor 4, Animals, Cells, Cultured, Genetic Vectors, Blotting, Southern, Lentivirus, Proto-Oncogene Proteins c-myc, Reverse Transcriptase Polymerase Chain Reaction, Pluripotent Stem Cells, Octamer Transcription Factor-3, Mice, Kruppel-Like Transcription Factors, SOXB1 Transcription Factors

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Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette

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