The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette
Open accessAbstract
Induced pluripotent stem (iPS) cells can be generated using retroviral vectors expressing Oct4, Klf4, Sox2, and cMyc. Most prior studies have required multiple retroviral vectors for reprogramming, resulting in high numbers of genomic integrations in iPS cells and limiting their use for therapeutic applications. Here we describe the use of a single lentiviral vector expressing a “stem cell cassette” composed of the four transcription factors and a combination of 2A peptide and internal ribosome entry site technology, generating iPS cells from postnatal fibroblasts. iPS cells generated in this manner display embryonic stem cell-like morphology, express stem cell markers, and exhibit in vivo pluripotency, as evidenced by their ability to differentiate in teratoma assays and their robust contribution to mouse chimeras. Combining all factors into a single transcript achieves the most efficient reprogramming system to date and allows derivation of iPS cells with a single viral integration. The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes.
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“Polycistronic technology reduces the requirement for multiple viral vectors to deliver four different transcription factors, cutting this down to a single-stem cell cassette [101].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [101]
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“Fibroblasts from four previously diagnosed FAP individuals were obtained from Coriell and reprogrammed using the STEMCCA system to generate three independent iPSC lines, as described [12, 18]”
From Modeling APC mutagenesis and familial adenomatous polyposis using human iPS cells (2018) · cites this paper as [18]
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Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Generation of germline-competent induced pluripotent stem cells
Okita, K., Ichisaka, T., & Yamanaka, S. (2007) · Nature · doi:10.1038/nature05934 · Cited by 4,438
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Mikkelsen, T. S., Ku, M., Jaffe, D. B., Issac, B., Lieberman, E., Giannoukos, G., Alvarez, P., Brockman, W., Kim, T.-K., Koche, R. P., et al. (2007) · Nature · doi:10.1038/nature06008 · Cited by 4,129
Niwa, H., Miyazaki, J., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,904
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
Wernig, M., Meissner, A., Foreman, R. K., Brambrink, T., Ku, M., Hochedlinger, K., Bernstein, B., & Jaenisch, R. (2007) · Nature · doi:10.1038/nature05944 · Cited by 2,732
Self-citationGenome-scale DNA methylation maps of pluripotent and differentiated cells
Meissner, A., Mikkelsen, T. S., Gu, H., Wernig, M., Hanna, J., Sivachenko, A., Zhang, X., Bernstein, B. E., Nusbaum, C., Jaffe, D. B., et al. (2008) · Nature · doi:10.1038/nature07107 · Cited by 2,536
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
Okita, K., Nakagawa, M., Hong, H., Ichisaka, T., & Yamanaka, S. (2008) · Science · doi:10.1126/science.1164270 · Cited by 2,056
Murry, C. E., & Keller, G. M. (2008) · Cell · doi:10.1016/j.cell.2008.02.008 · Cited by 1,760
Maherali, N., Sridharan, R., Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu, J., Jaenisch, R., et al. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.05.014 · Cited by 1,725
Self-citationInduced pluripotent stem cells generated without viral integration
Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G., & Hochedlinger, K. (2008) · Science · doi:10.1126/science.1162494 · Cited by 1,683
Self-citationTreatment of Sickle Cell Anemia Mouse Model with iPS Cells Generated from Autologous Skin
Hanna, J. H., Wernig, M., Markoulaki, S., Sun, C., Meissner, A., Cassady, J. P., Beard, C., Brambrink, T., Wu, L.-C., Townes, T. M., et al. (2007) · Science · doi:10.1126/science.1152092 · Cited by 1,521
Dissecting direct reprogramming through integrative genomic analysis
Mikkelsen, T. S., Hanna, J. H., Zhang, X., Ku, M., Wernig, M., Schorderet, P., Bernstein, B., Jaenisch, R., Lander, E. S., & Meissner, A. (2008) · Nature · doi:10.1038/nature07056 · Cited by 1,515
Wernig, M., Zhao, J.-P., Pruszak, J., Hedlund, E., Fu, D., Soldner, F., Broccoli, V., Constantine‐Paton, M., Ole Isacson, A., & Jaenisch, R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801677105 · Cited by 1,205
Szymczak, A. L., Workman, C. J., Wang, Y., Vignali, K. M., Dilioglou, S., Vanin, E. F., & Vignali, D. A. A. (2004) · Nature Biotechnology · doi:10.1038/nbt957 · Cited by 1,059
Sequential Expression of Pluripotency Markers during Direct Reprogramming of Mouse Somatic Cells
Brambrink, T., Foreman, R. K., Welstead, G. G., Lengner, C. J., Wernig, M., Suh, H., & Jaenisch, R. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.01.004 · Cited by 839
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse
Stadtfeld, M., Maherali, N., Breault, D. T., & Hochedlinger, K. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.02.001 · Cited by 823
Self-citationA drug-inducible transgenic system for direct reprogramming of multiple somatic cell types
Wernig, M., Lengner, C. J., Hanna, J. H., Lodato, M. A., Steine, E. J., Foreman, R. K., Staerk, J., Markoulaki, S., & Jaenisch, R. (2008) · Nature Biotechnology · doi:10.1038/nbt1483 · Cited by 445
Small Increases in the Level of Sox2 Trigger the Differentiation of Mouse Embryonic Stem Cells
Kopp, J. L., Ormsbee, B. D., Desler, M., & Rizzino, A. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0951 · Cited by 369
Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection
Blelloch, R. H., Venere, M., Yen, J. S., & Ramalho‐Santos, M. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.08.008 · Cited by 278
Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer
Mostoslavsky, G., Fabian, A. J., Rooney, S., Alt, F. W., & Mulligan, R. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608130103 · Cited by 157
Self-citationChinnasamy, D., Milsom, M. D., Shaffer, J., Neuenfeldt, J., Shaaban, A. F., Margison, G. P., Fairbairn, L., & Chinnasamy, N. (2006) · Virology Journal · doi:10.1186/1743-422x-3-14 · Cited by 81
Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages
Pan, H., Mostoslavsky, G., Eruslanov, E. B., Kotton, D. N., & Kramnik, I. (2007) · Journal of Immunological Methods · doi:10.1016/j.jim.2007.09.009 · Cited by 41
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Kaji, K., Norrby, K., Paca, A., Mileikovsky, M., Mohseni, P., & Woltjen, K. (2009) · Nature · doi:10.1038/nature07864 · Cited by 1,296
Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency
Anokye‐Danso, F., Trivedi, C. M., Juhr, D., Gupta, M., Cui, Z., Tian, Y., Zhang, Y., Yang, W., Gruber, P. J., Epstein, J. A., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2011.03.001 · Cited by 1,244
The promise of induced pluripotent stem cells in research and therapy
Robinton, D. A., & Daley, G. Q. (2012) · Nature · doi:10.1038/nature10761 · Cited by 1,150
Zhang, G., & Tandon, A. (2012) · Scientific Reports · doi:10.1038/srep00415 · Cited by 1,039
A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells
Polo, J. M., Anderssen, E., Walsh, R. M., Schwarz, B. A., Nefzger, C. M., Lim, S. M., Borkent, M., Apostolou, E., Alaei, S., Cloutier, J., et al. (2012) · Cell · doi:10.1016/j.cell.2012.11.039 · Cited by 861
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Metadata
- Authors
- Sommer, C. A., Stadtfeld, M., Murphy, G. J., Hochedlinger, K., Kotton, D. N., & Mostoslavsky, G.
- Affiliations
- Boston University (US)
- Massachusetts General Hospital (US)
- Harvard University (US)
- Corresponding author
- Mostoslavsky, Gustavo
- ORCID
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-03-01
- Volume
- 27
- Issue
- 3
- Pages
- 543-549
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- PMC
- PMC4848035
- OpenAlex
- W2038815755
- Cited by
- 676
- References
- 24
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 16 · 2023: 15 · 2022: 18 · 2021: 23 · 2020: 30 · 2019: 35 · 2018: 42 · 2017: 41 · 2016: 41 · 2015: 49 · 2014: 61 · 2013: 65 · 2012: 66
- FWCI
- 21.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Stem Cells (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- V Foundation for Cancer Research
- National Institutes of Health (1dp2od003266-01)
- Harvard Stem Cell Institute
- National Heart, Lung, and Blood Institute (P01HL047049)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R21HD060308)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Keywords
- induced pluripotent stem cells, lentiviral vectors, Oct4, KLF4, SOX2, c-Myc, 2A peptide, internal ribosome entry site, fibroblast reprogramming, chromosomal integration, teratoma assay, mouse chimeras, pluripotency
- MeSH terms
- Kruppel-Like Factor 4, Animals, Cells, Cultured, Genetic Vectors, Blotting, Southern, Lentivirus, Proto-Oncogene Proteins c-myc, Reverse Transcriptase Polymerase Chain Reaction, Pluripotent Stem Cells, Octamer Transcription Factor-3, Mice, Kruppel-Like Transcription Factors, SOXB1 Transcription Factors
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