The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Short‐Term BMP‐2 Expression Is Sufficient for In Vivo Osteochondral Differentiation of Mesenchymal Stem Cells
Abstract
Currently available murine models to evaluate mesenchymal stem cell (MSC) differentiation are based on cell injection at ectopic sites such as muscle or skin. Due to the importance of environmental factors on the differentiation capacities of stem cells in vivo, we investigated whether the peculiar synovial/cartilaginous environment may influence the lineage specificity of bone morphogenetic protein (BMP)-2-engineered MSCs. To this aim, we used the C3H10T1/2-derived C9 MSCs that express BMP-2 under control of the doxycycline (Dox)-repressible promoter, Tet-Off, and showed in vitro, using the micropellet culture system that C9 MSCs kept their potential to differentiate toward chondrocytes. Implantation of C9 cells, either into the tibialis anterior muscles or into the joints of CB17-severe combined immunodeficient bg mice led to the formation of cartilage and bone filled with bone marrow as soon as day 10. However, no differentiation was observed after injection of naïve MSCs or C9 cells that were repressed to secrete BMP-2 by Dox addition. The BMP-2-induced differentiation of adult MSCs is thus independent of soluble factors present in the local environment of the synovial/cartilaginous tissues. Importantly, we demonstrated that a short-term expression of the BMP-2 growth factor is necessary and sufficient to irreversibly induce bone formation, suggesting that a stable genetic modification of MSCs is not required for stem cell-based bone/cartilage engineering.
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Cites
Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals
Djouad, F., Plence, P., Bony, C., Tropel, P., Apparailly, F., Sany, J., Noël, D., & Jorgensen, C. (2003) · Blood · doi:10.1182/blood-2003-04-1193 · Cited by 1,166
Self-citationClinical applications of angiogenic growth factors and their inhibitors
Ferrara, N., & Alitalo, K. (1999) · Nature Medicine · doi:10.1038/70928 · Cited by 1,004
Sekiya, I., Vuoristo, J. T., Larson, B. L., & Prockop, D. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052716199 · Cited by 648
Cited by
TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation
Chen, G., Deng, C., & Li, Y.-P. (2012) · International Journal of Biological Sciences · doi:10.7150/ijbs.2929 · Cited by 1,756
Wu, M., Chen, G., & Li, Y.-P. (2016) · Bone Research · doi:10.1038/boneres.2016.9 · Cited by 1,553
Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals
Djouad, F., Plence, P., Bony, C., Tropel, P., Apparailly, F., Sany, J., Noël, D., & Jorgensen, C. (2003) · Blood · doi:10.1182/blood-2003-04-1193 · Cited by 1,166
Self-citationEliopoulos, N., Stagg, J., Lejeune, L., Pommey, S., & Galipeau, J. (2005) · Blood · doi:10.1182/blood-2005-03-1004 · Cited by 558
Review of Signaling Pathways Governing MSC Osteogenic and Adipogenic Differentiation
James, A. W. (2013) · Scientifica · doi:10.1155/2013/684736 · Cited by 493
Metadata
- Authors
- Noël, D., Gazit, D., Bouquet, C., Apparailly, F., Bony, C., Plence, P., Millet, V., Turgeman, G., Perricaudet, M., Sany, J., & Jorgensen, C.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-01
- Volume
- 22
- Issue
- 1
- Pages
- 74-85
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2104916764
- Cited by
- 239
- References
- 3
- FWCI
- 7.63
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Noël, D., Gazit, D., Bouquet, C., Apparailly, F., Bony, C., Plence, P., Millet, V., Turgeman, G., Perricaudet, M., Sany, J., & Jorgensen, C. (2004). Short‐Term BMP‐2 Expression Is Sufficient for In Vivo Osteochondral Differentiation of Mesenchymal Stem Cells. STEM CELLS. https://doi.org/10.1634/stemcells.22-1-74
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