The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Derivation of Human Embryonic Stem Cells from Day‐8 Blastocysts Recovered after Three‐Step In Vitro Culture
Open accessAbstract
Human embryonic stem cells (hESCs) have been derived from the inner cell mass (ICM) of day 5-7 blastocysts and hold great promise for research into human developmental biology and the development of cell therapies for the treatment of human diseases. We report here that our novel three-step culture conditions successfully support the development of day-8 human blastocysts, which possess significantly (p <.01) more ICM cells than day-6 blastocysts. Plating of ICMs isolated from day-8 blastocysts resulted in the formation of a colony with hESC morphology from which a new hESC line (hES-NCL1) was derived. Our stem cell line is characterized by the expression of specific cell surface and gene markers: GTCM-2, TG343, TRA1-60, SSEA-4, alkaline phosphatase, OCT-4, NANOG, and REX-1. Cytogenetic analysis of the hESCs revealed that hES-NCL1 line has a normal female (46, XX) karyotype. The pluripotency of the cell line was confirmed by the formation of teratomas after injection into severely combined immunodeficient mice and spontaneous differentiation under in vitro conditions.
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Cites
He, J.-Q., Ma, Y., Lee, Y., Thomson, J. A., & Kamp, T. J. (2003) · Circulation Research · doi:10.1161/01.res.0000080317.92718.99 · Cited by 800
Cited by
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Wobus, A. M., & Boheler, K. R. (2005) · Physiological Reviews · doi:10.1152/physrev.00054.2003 · Cited by 782
Characterization and culture of human embryonic stem cells
Hoffman, L. M., & Carpenter, M. K. (2005) · Nature Biotechnology · doi:10.1038/nbt1102 · Cited by 465
Yu, J., & Thomson, J. A. (2008) · Genes & Development · doi:10.1101/gad.1689808 · Cited by 342
Armstrong, L., Tilgner, K., Saretzki, G., Atkinson, S. P., Stojkovic, M., Moreno, R., Przyborski, S., & Lako, M. (2010) · Stem Cells · doi:10.1002/stem.307 · Cited by 289
Self-citationYang, C., Przyborski, S., Cooke, M. J., Zhang, X., Stewart, R., Anyfantis, G., Atkinson, S. P., Saretzki, G., Armstrong, L., & Lako, M. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0677 · Cited by 136
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Metadata
- Authors
- Stojkovic, M., Lako, M., Stojkovic, P., Stewart, R., Przyborski, S., Armstrong, L., Evans, J., Herbert, M., Hyslop, L., Ahmad, S., Murdoch, A., & Strachan, T.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-09
- Volume
- 22
- Issue
- 5
- Pages
- 790-797
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2160077902
- Cited by
- 176
- References
- 1
- FWCI
- 7.98
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Derivation of Human Embryonic Stem Cells from Day‐8 Blastocysts Recovered after Three‐Step In Vitro Culture. https://app.cochat.ai/papers/10.1634/stemcells.22-5-790
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- Stojkovic, M., Lako, M., Stojkovic, P., Stewart, R., Przyborski, S., Armstrong, L., Evans, J., Herbert, M., Hyslop, L., Ahmad, S., Murdoch, A., & Strachan, T. (2004). Derivation of Human Embryonic Stem Cells from Day‐8 Blastocysts Recovered after Three‐Step In Vitro Culture. STEM CELLS. https://doi.org/10.1634/stemcells.22-5-790
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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