THE DEVELOPMENT OF A RADIOIMMUNOASSAY FOR BOVINE NEUROPHYSIN
Abstract
SUMMARY: A radioimmunoassay for bovine neurophysin is described. The assay has a lower limit of detection of approximately 0·5 ng/ml. Bovine neurophysin extracted and purified from acetone-dried posterior pituitary lobe powder was used for standardization, radio-iodination and immunization of rabbits. Some anti-neurophysin sera were also obtained from human subjects treated with Pitressin. Most of the antisera tested did not readily distinguish between the different neurophysin components isolated from bovine neurohypophysial tissue. The antisera cross-reacted with material extracted from whole pituitaries of a number of mammalian species, including man, pig, sheep, guinea-pig and rat, but not with the pituitary peptides from the cod or dogfish, or the egret. Other pituitary peptides, including oxytocin, vasopressin, corticotrophin, growth hormone and luteinizing hormone, did not interfere with the assay.
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An antibody might recognise elements unrelated to the antigen against which it was raised, and unrelated molecules might cross-react.
“In some cases, an antibody might recognise elements unrelated to the antigen against which it was raised. Antibodies are sensitive to differences in amino acid sequence, although they recognise the structure of a molecule, not its amino acid sequence per se, and so unrelated molecules might cross‐react 76, 79.”
From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [79]
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“Antibodies are sensitive to differences in amino acid sequence, although they recognise the structure of a molecule, not its amino acid sequence per se , and so unrelated molecules might cross-react (76,79).”
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Livett, B. G. (1975) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1975.tb34180.x · Cited by 13 · see citing works
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Evaluation of clinical disturbances of the hypothalamus
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- Authors
- MARTIN, M. J., CHARD, T., & LANDON, J.
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1972-03-01
- Volume
- 52
- Issue
- 3
- Pages
- 481-495
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W1983823469
- Cited by
- 26
- References
- 0
- Citations by year
- 2016: 1
- FWCI
- 17.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- neurophysin, radioimmunoassay, neurohypophysis, radioiodination, antisera, cross-reactivity, pituitary peptides, oxytocin, vasopressin, ACTH, growth hormone, luteinizing hormone, pitressin
- MeSH terms
- Adrenocorticotropic Hormone, Animals, Birds, Cattle, Cross Reactions, Electrophoresis, Disc, Fishes, Guinea Pigs, Humans, Iodine Isotopes, Luteinizing Hormone, Oxytocin, Pituitary Gland, Posterior, Pituitary Hormones, Posterior, Protein Binding, Proteins, Rabbits, Radioimmunoassay, Sharks, Sheep, Growth Hormone, Swine, Vasopressins, Rats
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