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Development of a microtitre plate enzyme immunoassay for the determination of progesterone
Abstract
A rapid, solid-phase microtitre plate enzyme immunoassay (EIA) for progesterone is described using progesterone 3-O-carboxymethyloxime–horseradish peroxidase as the label and an antiserum raised in rabbits to a progesterone 11α-hemisuccinyl–bovine serum albumin immunogen. A competitive reaction was used with a reaction time of 2 h. Antibody-bound and free steroid were separated in a simple washing step of the antibody-adsorbed well surface. 2,2′-Azino-di-(3-ethylbenzthiazoline sulphonic acid) diammonium salt was used as the substrate with a reaction time of 1 h. A lower limit of sensitivity of 0·25 pg/well was obtained with the response being linear (logit/log) through 1000 pg/well. Results obtained by EIA and radioimmunoassay in several species gave excellent agreement (r = 0·98). This assay system allows accurate determination of progesterone in plasma with good specificity, precision and accuracy, and is suitable for the rapid assessment of luteal function and reproductive status in both clinical and research situations in a wide variety of species. J. Endocr. (1984) 101, 41–49
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Cited by
Munro, C. J., Stabenfeldt, G. H., Cragun, J. R., Addiego, L. A., Overstreet, J. W., & Lasley, B. L. (1991) · Clinical Chemistry · doi:10.1093/clinchem/37.6.838 · Cited by 422
Self-citationGenetic Variation in Susceptibility to Endocrine Disruption by Estrogen in Mice
Spearow, J. L., Doemeny, P., Sera, R., Leffler, R., & Barkley, M. (1999) · Science · doi:10.1126/science.285.5431.1259 · Cited by 254
Watson, R., Munro, C., Edwards, K. L., Norton, V., Brown, J. L., & Walker, S. L. (2013) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2013.02.001 · Cited by 114
Self-citationReproductive biology of captive male cottontop tamarin monkeys as a function of social environment
Ginther, A. J., Ziegler, T. E., & Snowdon, C. T. (2001) · Animal Behaviour · doi:10.1006/anbe.2000.1587 · Cited by 77
Clark, F. E., Fitzpatrick, M., Hartley, A., King, A. J., Lee, T., Routh, A., Walker, S. L., & George, K. (2012) · Zoo Biology · doi:10.1002/zoo.20396 · Cited by 75
Metadata
- Authors
- Munro, C., & Stabenfeldt, G.
- Venue
- Journal of Endocrinology
- Publisher
- Bioscientifica
- Published
- 1984-04
- Volume
- 101
- Issue
- 1
- Pages
- 41-49
- ISSN
- 0022-0795 (ISSN-L)
- 1479-6805
- OpenAlex
- W2130600353
- Cited by
- 452
- References
- 0
- FWCI
- 15.10
- Type
- journal article
- Language
- en
- Open access
- closed
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- Munro, C., & Stabenfeldt, G. (1984). Development of a microtitre plate enzyme immunoassay for the determination of progesterone. Journal of Endocrinology. https://doi.org/10.1677/joe.0.1010041
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
