The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Corticosteroid-Dependent Sodium Transport in a Novel Immortalized Mouse Collecting Duct Principal Cell Line
Abstract
Abstract.: The final control of sodium balance takes place in the cortical collecting duct (CCD) of the nephron, where corticosteroid hormones regulate sodium reabsorption by acting through mineralocorticoid (MR) and/or glucocorticoid (GR) receptors. A clone of principal CCD cells (mpkCCD c14) has been established that is derived from a transgenic mouse (SV40 large T antigen under the control of the SV40 enhancer/L-type pyruvate kinase promoter). Cells grown on filters form polarized monolayers with high electrical transepithelial resistance (R T approximately 4700 Ω × cm 2) and potential difference (P D approximately -50 mV) and have an amiloride-sensitive electrogenic sodium transport, as assessed by the short-circuit current method (I sc approximately 11 μA/cm 2). Reverse transcription-PCR experiments using rat MR primers, [3 H]aldosterone, and [3 H]dexamethasone binding and competition studies indicated that the mpkCCD c14 cells exhibit specific MR and GR. Aldosterone increased I sc in a dose- (10-10 to 10-6 M) and time-dependent (2 to 72 h) manner, whereas corticosterone only transiently increased I sc (2 to 6 h). Consistent with the expression of 11β-hydroxysteroid dehydrogenase type 2, which metabolizes glucocorticoids to inactive 11-dehydroderivates, carbenoxolone potentiated the corticosterone-stimulated I sc. Aldosterone (5 × 10-7 M)-induced I sc (fourfold) was associated with a three- to fivefold increase in α-ENaC mRNA (but not in those for β- or γ-ENaC) and three- to 10-fold increases in α-ENaC protein synthesis. In conclusion, this new immortalized mammalian CCD clonal cell line has retained a high level of epithelial differentiation and sodium transport stimulated by aldosterone and therefore represents a useful mammalian cell system for identifying the genes controlled by aldosterone.
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Cites
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Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits
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Amiloride and its analogs as tools in the study of ion transport
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Self-citationEarly death due to defective neonatal lung liquid clearance in αENaC-deficient mice
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Self-citationAn epithelial serine protease activates the amiloride-sensitive sodium channel
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Epithelial Sodium Channels Are Activated by Furin-dependent Proteolysis
Hughey, R. P., Bruns, J. B., Kinlough, C. L., Harkleroad, K. L., Tong, Q., Carattino, M. D., Johnson, J. P., Stockand, J. D., & Kleyman, T. R. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.c400080200 · Cited by 377
Multiple aspects of mineralocorticoid selectivity
Farman, N., & Rafestin-Oblin, M.-E. (2001) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.2001.280.2.f181 · Cited by 268
Self-citationTranscriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq
Chen, L., Lee, J. W., Chou, C.-L., Nair, A. V., Battistone, M. A., Păunescu, T. G., Merkulova, M., Breton, S., Verlander, J. W., Wall, S. M., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1710964114 · Cited by 267
Gumz, M. L., Stow, L. R., Lynch, I. J., Greenlee, M. M., Rudin, A., Cain, B. D., Weaver, D. R., & Wingo, C. S. (2009) · Journal of Clinical Investigation · doi:10.1172/jci36908 · Cited by 216
Stow, L. R., Richards, J., Cheng, K.-Y., Lynch, I. J., Jeffers, L. A., Greenlee, M. M., Cain, B. D., Wingo, C. S., & Gumz, M. L. (2012) · Hypertension · doi:10.1161/hypertensionaha.112.190892 · Cited by 146
Metadata
- Authors
- BENS, M., VALLET, V., CLUZEAUD, F. C., PASCUAL-LETALLEC, L., KAHN, A., RAFESTIN-OBLIN, M. E., ROSSIER, B. C., & VANDEWALLE, A.
- Venue
- Journal of the American Society of Nephrology
- Published
- 1999-05
- Volume
- 10
- Issue
- 5
- Pages
- 923-934
- ISSN
- 1046-6673 (ISSN-L)
- OpenAlex
- W2122680576
- Cited by
- 287
- References
- 40
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- closed
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- BENS, M., VALLET, V., CLUZEAUD, F. C., PASCUAL-LETALLEC, L., KAHN, A., RAFESTIN-OBLIN, M. E., ROSSIER, B. C., & VANDEWALLE, A. (1999). Corticosteroid-Dependent Sodium Transport in a Novel Immortalized Mouse Collecting Duct Principal Cell Line. Journal of the American Society of Nephrology. https://doi.org/10.1681/asn.v105923
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