The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Epithelial Sodium Channels Are Activated by Furin-dependent Proteolysis
Open accessAbstract
Epithelial Na(+) channels (ENaCs) are activated by extracellular trypsin or by co-expression with channel-activating proteases, although there is no direct evidence that these proteases activate ENaC by cleaving the channel. We previously demonstrated that the alpha and gamma subunits of ENaC are cleaved during maturation near consensus sites for furin cleavage. Using site-specific mutagenesis of channel subunits, ENaC expression in furin-deficient cells, and furin-specific inhibitors, we now report that ENaC cleavage correlates with channel activity. Channel activity in furin-deficient cells was rescued by expression of furin. Our data provide the first example of a vertebrate ion channel that is a substrate for furin and whose activity is dependent on its proteolysis.
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Cites
Furin at the cutting edge: From protein traffic to embryogenesis and disease
Thomas, G. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm934 · Cited by 1,216
Kellenberger, S., & Schild, L. (2002) · Physiological Reviews · doi:10.1152/physrev.00007.2002 · Cited by 1,026
Molloy, S. S., Bresnahan, P. A., Leppla, S. H., Klimpel, K. R., & Thomas, G. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42016-9 · Cited by 689
An epithelial serine protease activates the amiloride-sensitive sodium channel
Vallet, V., Chraibi, A., Gaeggeler, H.-P., Horisberger, J.-D., & Rossier, B. C. (1997) · Nature · doi:10.1038/39329 · Cited by 514
Proteolytic Processing in the Secretory Pathway
Zhou, A., Webb, G., Zhu, X., & Steiner, D. F. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.30.20745 · Cited by 457
The heterotetrameric architecture of the epithelial sodium channel (ENaC)
Firsov, D. (1998) · The EMBO Journal · doi:10.1093/emboj/17.2.344 · Cited by 405
BENS, M., VALLET, V., CLUZEAUD, F. C., PASCUAL-LETALLEC, L., KAHN, A., RAFESTIN-OBLIN, M. E., ROSSIER, B. C., & VANDEWALLE, A. (1999) · Journal of the American Society of Nephrology · doi:10.1681/asn.v105923 · Cited by 287
Maturation of the Epithelial Na+ Channel Involves Proteolytic Processing of the α- and γ-Subunits
Hughey, R. P., Mueller, G. M., Bruns, J. B., Kinlough, C. L., Poland, P. A., Harkleroad, K. L., Carattino, M. D., & Kleyman, T. R. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m307003200 · Cited by 268
Self-citationJean, F., Stella, K., Thomas, L., Liu, G., Xiang, Y., Reason, A. J., & Thomas, G. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.13.7293 · Cited by 263
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Cited by
Nedd4-2 Induces Endocytosis and Degradation of Proteolytically Cleaved Epithelial Na+ Channels
Kabra, R., Knight, K. K., Zhou, R., & Snyder, P. M. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m708555200 · Cited by 86
Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity
Jella, K. K., Yu, L., Yue, Q., Friedman, D., Duke, B. J., & Alli, A. A. (2016) · PLOS ONE · doi:10.1371/journal.pone.0165763 · Cited by 81
Ubiquitin-specific Peptidase 8 (USP8) Regulates Endosomal Trafficking of the Epithelial Na+ Channel*
Zhou, R., Tomkovicz, V. R., Butler, P. L., Ochoa, L. A., Peterson, Z. J., & Snyder, P. M. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.425272 · Cited by 47
Metadata
- Authors
- Hughey, R. P., Bruns, J. B., Kinlough, C. L., Harkleroad, K. L., Tong, Q., Carattino, M. D., Johnson, J. P., Stockand, J. D., & Kleyman, T. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 279
- Issue
- 18
- Pages
- 18111-18114
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2058058212
- Cited by
- 377
- References
- 34
- FWCI
- 6.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hughey, R. P., Bruns, J. B., Kinlough, C. L., Harkleroad, K. L., Tong, Q., Carattino, M. D., Johnson, J. P., Stockand, J. D., & Kleyman, T. R. (2004). Epithelial Sodium Channels Are Activated by Furin-dependent Proteolysis. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.c400080200
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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