Aldh1l1-Cre/ERT2 is expressed in unintended cell types of the salivary gland, pancreas, and spleen.
Open accessAbstract
The Aldh1l1-Cre/ER T2 mouse is a widely used transgenic mouse model to conditionally express Cre recombinase in astrocytes of the central nervous system. Currently, no reports show whether the Cre recombinase activity, driven by the Aldh1l1 promoter, acts in cells outside of its intended astrocyte population. We crossed the Aldh1l1-Cre/ER T2 mouse with a TdTomato reporter mouse line, ROSA26:CAG-LSL-TdTomato, to generate a fluorescent reporter for Aldh1l1 promoter activity. Gross anatomical observations reveal strong TdTomato expression in the spleen and exocrine glands—the salivary gland and the pancreas. We find TdTomato expression, a reporter of Cre activity, specifically targets serous cells in the parotid, submandibular, sublingual glands, and pancreas along with fibroblast-like cells within the submandibular lymph nodes and spleen. Our data indicate that the Aldh1l1-Cre/ER T2 mouse model has unintended Cre recombinase activity in exocrine glands, which may influence biological and behavioral data.
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Aldh1l1 is also expressed in the liver, as evidenced by the plasma lipid profile of APOE4s2A mice.
“Aldh1l1 is also expressed in the liver65, as evidenced by the plasma lipid profile of APOE4s2A mice.”
From APOE4 to APOE2 allelic switching in mice improves Alzheimer’s disease-related metabolic signatures, neuropathology and cognition (2025) · cites this paper as [65]
The Description reports Aldh1l1 promoter activity in the salivary gland, lymph nodes, pancreas, and spleen, but does not address the liver. Searches for liver or hepatic expression and for the APOE4s2A plasma lipid profile found no supporting passage in our copy. The citation is attached specifically to the liver-expression claim. (LLM, conf=0.76)
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- Authors
- Nemeth, D., Luqman, N., Chen, L., & Quan, N.
- Affiliations
- Florida Atlantic University (US)
- Venue
- microPublication Biology
- Publisher
- microPublication Biology
- Published
- 2023
- Volume
- 2023
- PMC
- PMC10238921
- OpenAlex
- W4379376698
- Cited by
- 6
- References
- 15
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 1
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by
- Locations
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- microPublication
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS116914)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- salivary glands, pancreas, spleen, exocrine glands, conditional knockout mice
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