CoChat
Sign Up
Cited by 13

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs)

Colasuonno, Fiorella, Borghi, Rossella, Niceforo, Alessia, Muzzi, Maurizio, Bertini, Enrico, Di Giulio, Andrea, Moreno, Sandra, Compagnucci, Claudia·2017·Aging
DOI 10.18632/aging.101309PMC PMC5680563
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.18632/aging.101309

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Bright field images, acquired at the inverted microscope of y-iPSCs (a), ma-iPSCs (b,d) and a-iPSCs (c,e). Scale bars, 20 μm (a-c), 10 μm (d,e).
Figure 1 Bright field images, acquired at the inverted microscope of y-iPSCs (a), ma-iPSCs (b,d) and a-iPSCs (c,e). Scale bars, 20 μm (a-c), 10 μm (d,e).CC BY · PubMed Central
Figure 2 FIB/SEM micrographs of y-iPSCs (a,b), ma-iPSCs (c,d) and a-iPSCs (e,f). Images have been electronically colored to highlight nuclei (in blue), mitochondria (in pink), and autophagosomes (in green). Arrowheads, gap junctions; asterisks, tight junctions; G, Golgi apparatus; RER, rough endoplasmic reticulum.
Figure 2 FIB/SEM micrographs of y-iPSCs (a,b), ma-iPSCs (c,d) and a-iPSCs (e,f). Images have been electronically colored to highlight nuclei (in blue), mitochondria (in pink), and autophagosomes (in green). Arrowheads, gap junctions; asterisks, tight junctions; G, Golgi apparatus; RER, rough endoplasmic reticulum.CC BY · PubMed Central
Figure 3 FIB/SEM micrographs of y-, ma- and a-iPSCs showing changes in mitochondrial structure during culturing time. Mitochondria with disorganized cristae are indicated by the asterisks in ma- and a-iPSCs. N, nuclei Scale bars, 1μm.
Figure 3 FIB/SEM micrographs of y-, ma- and a-iPSCs showing changes in mitochondrial structure during culturing time. Mitochondria with disorganized cristae are indicated by the asterisks in ma- and a-iPSCs. N, nuclei Scale bars, 1μm.CC BY · PubMed Central
Figure 4 FIB/SEM images showing ultrastructural details of ma- (a-c) and a-iPSCs (d-f). Arrows in b,c,e indicate autophagosomes containing mitochondria (m) or other partially digested cytoplasmic material. E, exosomes; N, nuclei; (TGN, trans-Golgi network; RER, rough endoplasmatic reticulum).
Figure 4 FIB/SEM images showing ultrastructural details of ma- (a-c) and a-iPSCs (d-f). Arrows in b,c,e indicate autophagosomes containing mitochondria (m) or other partially digested cytoplasmic material. E, exosomes; N, nuclei; (TGN, trans-Golgi network; RER, rough endoplasmatic reticulum).CC BY · PubMed Central
Figure 5 Autophagy is increased in ma-iPSCs when compared with y- and a-iPSCs. Western blot analysis of LC3 I/II, and alpha-tubulin as loading control is shown. Bar graph shows the LC3–II/I ratio and represents the mean± SD of three experiments.
Figure 5 Autophagy is increased in ma-iPSCs when compared with y- and a-iPSCs. Western blot analysis of LC3 I/II, and alpha-tubulin as loading control is shown. Bar graph shows the LC3–II/I ratio and represents the mean± SD of three experiments.CC BY · PubMed Central
Figure 6 Confocal microscopic images of y-, ma- and a-iPSCs marked with p62 (in red) and Hoechst (in blue) showing abundant granular p62 immunopositivity in ma-iPSCs and in a-iPSCs when compared with y-iPSCs.
Figure 6 Confocal microscopic images of y-, ma- and a-iPSCs marked with p62 (in red) and Hoechst (in blue) showing abundant granular p62 immunopositivity in ma-iPSCs and in a-iPSCs when compared with y-iPSCs.CC BY · PubMed Central
Figure 7 Confocal microscopy of y-, ma- and a-iPSCs immunostained with Beclin1 (in green), Lamp2 (in red) and Hoechst (in blue). In ma-iPSCs the central cell shows a granular distribution of Lamp2, while the arrow indicates a cell with a perinuclear staining similar to those observed in a-iPSCs.
Figure 7 Confocal microscopy of y-, ma- and a-iPSCs immunostained with Beclin1 (in green), Lamp2 (in red) and Hoechst (in blue). In ma-iPSCs the central cell shows a granular distribution of Lamp2, while the arrow indicates a cell with a perinuclear staining similar to those observed in a-iPSCs.CC BY · PubMed Central
Figure 8 Confocal microscopy of y- and a-iPSCs stained with anti-p62 antibody (in red), anti-Beclin1 (in green), Lamp2 (in red) and Hoechst (in blue), treated with rapamycin alone or in combination with bafilomycin.
Figure 8 Confocal microscopy of y- and a-iPSCs stained with anti-p62 antibody (in red), anti-Beclin1 (in green), Lamp2 (in red) and Hoechst (in blue), treated with rapamycin alone or in combination with bafilomycin.CC BY · PubMed Central
Figure 9 Schematic diagram of biological features observed in y-iPSCs, ma- (following 6 months of culture) and a-iPSCs (In particular, we highlighted the mitochondrial disruption, the progressive loss of cell- cell contacts, the presence of autophagosomes and extracellular vesicles with aging. m, mitochondria; N, nucleus; E, extracellular vesicles; A, autophagosomes.
Figure 9 Schematic diagram of biological features observed in y-iPSCs, ma- (following 6 months of culture) and a-iPSCs (In particular, we highlighted the mitochondrial disruption, the progressive loss of cell- cell contacts, the presence of autophagosomes and extracellular vesicles with aging. m, mitochondria; N, nucleus; E, extracellular vesicles; A, autophagosomes.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

  • Feedback between p21 and reactive oxygen production is necessary for cell senescence

    Passos, J. F., Nelson, G., Wang, C., Richter, T., Simillion, C., Proctor, C. J., Miwa, S., Olijslagers, S., Hallinan, J., Wipat, A., et al. (2010) · Molecular Systems Biology · doi:10.1038/msb.2010.5 · Cited by 1,019 · cited 1×

    “These results are in line with previous data from ours and other Authors’ groups reporting. increased mitochondrial biogenesis [7, 23], and are possibly due to adaptation of the cells to the culture environment (5% CO 2 and 21% O 2 ).”

Research works printed but not linked: 36

  1. Yamanaka, 2006, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors., Cell, 12, 663, 10.1016/j.cell.2006.07.024 · DOI · Paper page
  2. Bertini, 2014, In vitro neurogenesis: development and functional implications of iPSC technology., Cell Mol Life Sci, 7, 1623, 10.1007/s00018-013-1511-1 · DOI
  3. Prigione, 2014, Mitochondrial function in pluripotent stem cells and cellular reprogramming., Gerontology, 6, 174, 10.1159/000355050 · DOI
  4. Zavazava, 2015, Human iPS cell-derived insulin producing cells form vascularized organoids under the kidney capsules of diabetic mice., PLoS One, 1, e0116582, 10.1371/journal.pone.0116582 · DOI
  5. Meier, 2016, Ultrastructural demonstration of Cx43 gap junctions in induced pluripotent stem cells from human cord blood., Histochem Cell Biol, 14, 529, 10.1007/s00418-016-1469-9 · DOI
  6. Zhang, 2013, Genetic and epigenetic variations in iPSCs: potential causes and implications for application., Cell Stem Cell, 1, 149, 10.1016/j.stem.2013.07.001 · DOI
  7. Compagnucci, 2014, Aged iPSCs display an uncommon mitochondrial appearance and fail to undergo in vitro neurogenesis., Aging (Albany NY), 6, 1094, 10.18632/aging.100708 · DOI
  8. Compagnucci, 2017, Aged induced pluripotent stem cell (iPSCs) as a new cellular model for studying premature aging., Aging (Albany NY), 9, 1453, 10.18632/aging.101248 · DOI
  9. Subramaniam, 2015, Focused ion beams in biology., Nat Methods, 1, 1021, 10.1038/nmeth.3623 · DOI
  10. Humbel, 2014, Focused ion beam scanning electron microscopy in biology., J Microsc, 25, 109, 10.1111/jmi.12127 · DOI
  11. Stoorvogel, 2013, Extracellular vesicles: exosomes, microvesicles, and friends., J Cell Biol, 20, 373, 10.1083/jcb.201211138 · DOI · Paper page
  12. Johansen, 2007, p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy., J Biol Chem, 28, 24131, 10.1074/jbc.M702824200 · DOI · Paper page
  13. Yamanaka, 2016, A decade of transcription factor-mediated reprogramming to pluripotency., Nat Rev Mol Cell Biol, 1, 183, 10.1038/nrm.2016.8 · DOI
  14. Chhabra, 2017, Derivation of Human Induced Pluripotent Stem Cell (iPSC) Lines and Mechanism of Pluripotency: Historical Perspective and Recent Advances., Stem Cell Rev, 10.1007/s12015-017-9766-9 · DOI
  15. Martin, 2017, Genome stability of programmed stem cell products., Adv Drug Deliv Rev, 10.1016/j.addr.2017.09.004 · DOI
  16. Cibelli, 2010, Mitochondrial rejuvenation after induced pluripotency., PLoS One, 5, e14095, 10.1371/journal.pone.0014095 · DOI
  17. Romani, 2012, Functional structure of antennal sensilla in the myrmecophilous beetle Paussus favieri (Coleoptera, Carabidae, Paussini)., Micron, 4, 705, 10.1016/j.micron.2011.10.013 · DOI
  18. Romani, 2015, Functional anatomy of the explosive defensive system of bombardier beetles (Coleoptera, Carabidae, Brachininae)., Arthropod Struct Dev, 4, 468, 10.1016/j.asd.2015.08.013 · DOI
  19. Tang, 2015, Exosomes/microvesicles from induced pluripotent stem cells deliver cardioprotective miRNAs and prevent cardiomyocyte apoptosis in the ischemic myocardium., Int J Cardiol, 19, 61, 10.1016/j.ijcard.2015.05.020 · DOI
  20. Yang, 2017, Exosomes Generated From iPSC-Derivatives: New Direction for Stem Cell Therapy in Human Heart Diseases., Circ Res, 12, 407, 10.3892/ol.2017.5824 · DOI
  21. Jiang, 2017, Role of stem cell-derived exosomes in cancer., Oncol Lett, 1, 2855, 10.1161/CIRCRESAHA.116.309307 · DOI
  22. Brenner, 2007, Mitochondria in stem cells., Mitochondrion, 7, 289, 10.1016/j.mito.2007.05.002 · DOI
  23. Evans, 2012, The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming., Cell Res, 2, 168, 10.1038/cr.2011.177 · DOI
  24. Teitell, 2012, Metabolic regulation in pluripotent stem cells during reprogramming and self-renewal., Cell Stem Cell, 1, 589, 10.1016/j.stem.2012.10.005 · DOI
  25. Narendra, 2011, Mechanisms of mitophagy., Nat Rev Mol Cell Biol, 1, 9, 10.1038/nrm3028 · DOI · Paper page
  26. Popa-Wagner, 2017, Autophagy in aging and disease., Rom J Morphol Embryol, 5, 27
  27. Ramalho-Santos, 2011, Open chromatin in pluripotency and reprogramming., Nat Rev Mol Cell Biol, 1, 36, 10.1038/nrm3036 · DOI
  28. Menendez, 2016, Mitophagy-driven mitochondrial rejuvenation regulates stem cell fate., Aging (Albany NY), 8, 1330, 10.18632/aging.100976 · DOI
  29. Wong, 2016, Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells., Aging (Albany NY), 8, 945, 10.18632/aging.100950 · DOI
  30. Pébay, 2017, Mitochondrial replacement in an iPSC model of Leber’s hereditary optic neuropathy., Aging (Albany NY), 9, 1341, 10.18632/aging.101231 · DOI
  31. Nakatsuji, 2017, Friedreich’s ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency., Aging (Albany NY), 9, 1440, 10.18632/aging.101247 · DOI
  32. Fambrough, 1997, Different steady state subcellular distributions of the three splice variants of lysosome-associated membrane protein LAMP-2 are determined largely by the COOH-terminal amino acid residue., J Cell Biol, 13, 1161, 10.1083/jcb.137.5.1161 · DOI
  33. Bonifacino, 2005, Role of the endocytic machinery in the sorting of lysosome-associated membrane proteins., Mol Biol Cell, 1, 4231, 10.1091/mbc.E05-03-0213 · DOI
  34. Kalraiya, 2005, Altered melanoma cell surface glycosylation mediates organ specific adhesion and metastasis via lectin receptors on the lung vascular endothelium., Clin Exp Metastasis, 2, 11, 10.1007/s10585-005-2036-2 · DOI
  35. Kiyama, 2017, Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM., J Comp Neurol, 52, 2535, 10.1002/cne.24213 · DOI
  36. Nath, 2014, Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission., J Virol, 8, 10327, 10.1128/JVI.00788-14 · DOI

Cited by

Sorted by citations

See all 13

Metadata

Authors
Colasuonno, F., Borghi, R., Niceforo, A., Muzzi, M., Bertini, E., Di Giulio, A., Moreno, S., & Compagnucci, C.
Affiliations
  • Department of Science, LIME, University "Roma Tre", Rome 00146, Italy
  • Department of Neuroscience, Unit of Neuromuscular and Neurodegenerative Diseases, Laboratory of Molecular Medicine, Bambino Gesu' Children's Research Hospital, IRCCS, Rome 00146, Italy
Venue
Aging
Publisher
Impact Journals, LLC
Published
2017-10-23
Volume
9
Issue
10
Pages
2209-2222
ISSN
1945-4589
DOI
10.18632/aging.101309
PMC
PMC5680563
Cited by
13
References
1
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review