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cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53

Skah, Seham, Richartz, Nina, Duthil, Eva, Gilljam, Karin M., Bindesbøll, Christian, Naderi, Elin Hallan, Eriksen, Agnete B., Ruud, Ellen, Dirdal, Marta M., Simonsen, Anne, Blomhoff, Heidi Kiil·2018·Oncotarget
DOI 10.18632/oncotarget.25758PMC PMC6084393
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Figure 1 cAMP signaling enhances the DNA damage-induced LC3-II/LC3-I ratio (A and B) REH cells (0.6×106 cells/ml) were incubated in the presence or absence of forskolin (Forsk, 60 μM) for 45 min prior to irradiation (IR, 10Gy), and total lysates were subjected to immunoblot analyses with antibodies against LC3B or calnexin (CANX). The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. (A) When indicated, BafA1 (2 nM) was added from the start of the cultures. The cells were harvested at the indicated time points after IR, and one representative Western blot of three is shown. (B) Left panel: The cells were harvested 24 hours after IR, and BafA1 (2 nM) was added for the last 4 hours, as indicated. One representative Western blot of 8 is shown. Right panel: Ratios of the LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=8. *p< 0.05 (paired t test).
Figure 1 cAMP signaling enhances the DNA damage-induced LC3-II/LC3-I ratio (A and B) REH cells (0.6×106 cells/ml) were incubated in the presence or absence of forskolin (Forsk, 60 μM) for 45 min prior to irradiation (IR, 10Gy), and total lysates were subjected to immunoblot analyses with antibodies against LC3B or calnexin (CANX). The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. (A) When indicated, BafA1 (2 nM) was added from the start of the cultures. The cells were harvested at the indicated time points after IR, and one representative Western blot of three is shown. (B) Left panel: The cells were harvested 24 hours after IR, and BafA1 (2 nM) was added for the last 4 hours, as indicated. One representative Western blot of 8 is shown. Right panel: Ratios of the LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=8. *p< 0.05 (paired t test).CC BY · PubMed Central
Figure 2 PKA- and doxorubicin-mediated autophagy (A and B) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B. When indicated, the cells were treated with or without 8CPT-cAMP (8CPT, 200μM) 45 min prior to IR (panel A) or with 150 nM doxorubicin (Doxo) 45 min after adding forskolin (panel B). Left panels: One representative Western blot of three independent experiments is shown. The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. Right panels: Ratios of the LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=3. *p<0.05 (paired t test).
Figure 2 PKA- and doxorubicin-mediated autophagy (A and B) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B. When indicated, the cells were treated with or without 8CPT-cAMP (8CPT, 200μM) 45 min prior to IR (panel A) or with 150 nM doxorubicin (Doxo) 45 min after adding forskolin (panel B). Left panels: One representative Western blot of three independent experiments is shown. The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. Right panels: Ratios of the LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=3. *p<0.05 (paired t test).CC BY · PubMed Central
Figure 3 Immunocytochemistry of LC3-puncta (A and B) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B, with addition of BafA1 to all samples. The cells were subjected to immunocytochemistry for the detection of LC3 puncta by confocal microscopy, and the cells were co-stained with Hoechst for visualization of the nuclei. (A) One representative of three independent experiments is shown. Scale bars = 10μm. (B) The number of LC3 puncta per cell from three independent experiments were quantified, counting at least 30 cells. The data represent the mean +/- SEM, n = 30. The numbers of small and large puncta are indicated.
Figure 3 Immunocytochemistry of LC3-puncta (A and B) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B, with addition of BafA1 to all samples. The cells were subjected to immunocytochemistry for the detection of LC3 puncta by confocal microscopy, and the cells were co-stained with Hoechst for visualization of the nuclei. (A) One representative of three independent experiments is shown. Scale bars = 10μm. (B) The number of LC3 puncta per cell from three independent experiments were quantified, counting at least 30 cells. The data represent the mean +/- SEM, n = 30. The numbers of small and large puncta are indicated.CC BY · PubMed Central
Figure 4 cAMP signaling enhances autophagic flux in REH cells (A-E) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B. CYTO-ID staining was performed 24 hours after IR – if not otherwise indicated, and the staining intensity was analyzed by flow cytometry and normalized to untreated (Ctrl) cells. (A) Confocal images of IR/forskolin-treated cells stained with CYTO-ID, (B) The co-localization between the autophagosomal marker CYTO-ID and LC3-puncta was analyzed by confocal imaging of IR/forskolin-treated cells. (C) The cells were pretreated with the ULK1 inhibitor MRT68921 (100 nM) for 30 min prior to adding forskolin, and the cells were irradiated after another 45 min. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=3. *p=0.05 (paired t test). (D) The cells were stained with CYTO-ID at the indicated time points, and the fluorescence intensity was analyzed by flow cytometry. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=5. *p<0.05 (paired t test). (E) Cells were treated with or without 2 nM BafA1for the last 4 hours of the 24 hours incubation. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=5. *p<0.05 (paired t test). (F) The effect of IR and forskolin on relative autophagic flux was quantified by measuring the degradation of long-lived proteins as described in Materials and Methods. The data represent the mean +/- SEM, n=3, and the values are normalized to the degradation in untreated (Ctrl) cells. *p<0.05 (paired t test).
Figure 4 cAMP signaling enhances autophagic flux in REH cells (A-E) REH cells were treated with or without forskolin, IR and BafA1 as described in Figure 1B. CYTO-ID staining was performed 24 hours after IR – if not otherwise indicated, and the staining intensity was analyzed by flow cytometry and normalized to untreated (Ctrl) cells. (A) Confocal images of IR/forskolin-treated cells stained with CYTO-ID, (B) The co-localization between the autophagosomal marker CYTO-ID and LC3-puncta was analyzed by confocal imaging of IR/forskolin-treated cells. (C) The cells were pretreated with the ULK1 inhibitor MRT68921 (100 nM) for 30 min prior to adding forskolin, and the cells were irradiated after another 45 min. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=3. *p=0.05 (paired t test). (D) The cells were stained with CYTO-ID at the indicated time points, and the fluorescence intensity was analyzed by flow cytometry. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=5. *p<0.05 (paired t test). (E) Cells were treated with or without 2 nM BafA1for the last 4 hours of the 24 hours incubation. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=5. *p<0.05 (paired t test). (F) The effect of IR and forskolin on relative autophagic flux was quantified by measuring the degradation of long-lived proteins as described in Materials and Methods. The data represent the mean +/- SEM, n=3, and the values are normalized to the degradation in untreated (Ctrl) cells. *p<0.05 (paired t test).CC BY · PubMed Central
Figure 5 cAMP signaling enhances CYTO-ID staining in NALM-6 and in primary BCP-ALL cells (A) NALM-6 cells (0.6×106 cells/ml) were treated with or without forskolin (Forsk, 60μM) for 45 min prior to irradiation (IR, 5Gy). BafA1 (2 nM) was added to the cell cultures for the last 4 hours of the 24 hours incubation, before the cells were stained with CYTO-ID and analyzed for fluorescence intensity by flow cytometry. The data represent the mean CYTO-ID fluorescence intensity ± SEM, n=4. *p<0.05 (paired t test). (B) Primary leukemic blasts (0.6 x106 cells/ml) from three patients diagnosed with BCP-ALL were treated with or without Forsk, IR and BafA1 as described in panel A.
Figure 5 cAMP signaling enhances CYTO-ID staining in NALM-6 and in primary BCP-ALL cells (A) NALM-6 cells (0.6×106 cells/ml) were treated with or without forskolin (Forsk, 60μM) for 45 min prior to irradiation (IR, 5Gy). BafA1 (2 nM) was added to the cell cultures for the last 4 hours of the 24 hours incubation, before the cells were stained with CYTO-ID and analyzed for fluorescence intensity by flow cytometry. The data represent the mean CYTO-ID fluorescence intensity ± SEM, n=4. *p<0.05 (paired t test). (B) Primary leukemic blasts (0.6 x106 cells/ml) from three patients diagnosed with BCP-ALL were treated with or without Forsk, IR and BafA1 as described in panel A.CC BY · PubMed Central
Figure 6 cAMP-mediated inhibition of DNA damage-induced cell death involves autophagy REH cells were treated with or without forskolin and irradiation as described in Figure 1. When indicated, BafA1 (2 nM) (A) or the ULK1 inhibitor MRT68921 (100 nM) (B) was present in the cell cultures throughout the experiments to block autophagy. The percentage of PI-positive cells was analyzed by flow cytometry 24 hours or 48 hours after IR, as indicated. The results are presented as the mean ± SEM, n=6. *p<0.05 (paired t test).
Figure 6 cAMP-mediated inhibition of DNA damage-induced cell death involves autophagy REH cells were treated with or without forskolin and irradiation as described in Figure 1. When indicated, BafA1 (2 nM) (A) or the ULK1 inhibitor MRT68921 (100 nM) (B) was present in the cell cultures throughout the experiments to block autophagy. The percentage of PI-positive cells was analyzed by flow cytometry 24 hours or 48 hours after IR, as indicated. The results are presented as the mean ± SEM, n=6. *p<0.05 (paired t test).CC BY · PubMed Central
Figure 7 The effects of IR and cAMP signaling on the subcellular localization of p53 REH cells were treated with or without forskolin and IR as described in Figure 1. (A) 4 hours after IR, the cells were subjected to immunocytochemistry for the detection of the subcellular localization of p53 by confocal microscopy, and the cells were co-stained with Hoechst for the visualization of nuclei. One representative of three independent experiments is shown. Scale bars = 10μm. (B) Quantification of the p53 fluorescence intensity of cells from three experiments, analyzing at least 30 cells. The data represent the mean +/-SEM, n=30. *p<0.05, (paired t test). (C) Subcellular fractionation of REH cells (20×106) was performed as described in Materials and Methods 4 hours after IR. The cytoplasmic and nuclear fractions were each subjected to immunoblot analyses of p53 expression. The numbers indicated below the p53 image represents the p53 signal intensity relative to the CANX signal, normalized to the ratio in untreated (Ctrl) cells. Left panel: One of three representative Western blots. Right panel: Quantification of the p53 signal in Western blots, normalized to the loading control GAPDH. The data represent the mean +/-SEM, n=3. *p<0.05 (paired t test).
Figure 7 The effects of IR and cAMP signaling on the subcellular localization of p53 REH cells were treated with or without forskolin and IR as described in Figure 1. (A) 4 hours after IR, the cells were subjected to immunocytochemistry for the detection of the subcellular localization of p53 by confocal microscopy, and the cells were co-stained with Hoechst for the visualization of nuclei. One representative of three independent experiments is shown. Scale bars = 10μm. (B) Quantification of the p53 fluorescence intensity of cells from three experiments, analyzing at least 30 cells. The data represent the mean +/-SEM, n=30. *p<0.05, (paired t test). (C) Subcellular fractionation of REH cells (20×106) was performed as described in Materials and Methods 4 hours after IR. The cytoplasmic and nuclear fractions were each subjected to immunoblot analyses of p53 expression. The numbers indicated below the p53 image represents the p53 signal intensity relative to the CANX signal, normalized to the ratio in untreated (Ctrl) cells. Left panel: One of three representative Western blots. Right panel: Quantification of the p53 signal in Western blots, normalized to the loading control GAPDH. The data represent the mean +/-SEM, n=3. *p<0.05 (paired t test).CC BY · PubMed Central
Figure 8 p53 is involved in IR-, but not in cAMP-induced autophagy (A-D) REH cells (4×106 cells) were transfected with siRNA against p53 (or with scrambled siRNA as control) as described in Materials and Methods, and after 12 hours the cells were treated with or without forskolin and IR as described in Figure 1. (A) Knock-down of p53 by siRNA demonstrated by Western blot analyses of untreated (Ctrl) and irradiated (IR) cells. Signal intensities of p53 relative to GAPDH, normalized to Ctrl, are indicated as numbers below the p53 images. (B-D) BafA1 (2 nM) was added for the last 4 hours of the 24 hours incubations. (B) 24 hours after IR, the cells were harvested for Western blot analyses of LC3-II/I ratios, and one representative of 4 independent experiments is shown. The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. (C) Quantifications of the Western blots in panel B, presented as the ratios of LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=4. *p<0.05 (paired t test). (D) The same cells as in panel A were subjected to CYTO-ID staining, and the fluorescence intensity was analysed by flow cytometry 24 hours after IR. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=4. *p<0.05 (paired t test).
Figure 8 p53 is involved in IR-, but not in cAMP-induced autophagy (A-D) REH cells (4×106 cells) were transfected with siRNA against p53 (or with scrambled siRNA as control) as described in Materials and Methods, and after 12 hours the cells were treated with or without forskolin and IR as described in Figure 1. (A) Knock-down of p53 by siRNA demonstrated by Western blot analyses of untreated (Ctrl) and irradiated (IR) cells. Signal intensities of p53 relative to GAPDH, normalized to Ctrl, are indicated as numbers below the p53 images. (B-D) BafA1 (2 nM) was added for the last 4 hours of the 24 hours incubations. (B) 24 hours after IR, the cells were harvested for Western blot analyses of LC3-II/I ratios, and one representative of 4 independent experiments is shown. The numbers indicated below the LC3 images represent the LC3-II/LC3-I signal ratios relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. (C) Quantifications of the Western blots in panel B, presented as the ratios of LC3-II/LC3-I signal intensities relative to the CANX signals, normalized to the ratio in untreated (Ctrl) cells. The data represent the mean +/- SEM, n=4. *p<0.05 (paired t test). (D) The same cells as in panel A were subjected to CYTO-ID staining, and the fluorescence intensity was analysed by flow cytometry 24 hours after IR. The data represent the mean CYTO-ID fluorescence intensity +/- SEM, n=4. *p<0.05 (paired t test).CC BY · PubMed Central
Figure 9 Proposed model for cAMP-mediated survival of DNA damage-induced BCP-ALL cells According to the model, cAMP-mediated survival of BCP-ALL cells exposed to DNA damage involves reduced p53-mediated apoptosis as well as p53-independent enhancement of autophagy. We suggest that autophagy needs to exceed a certain threshold in order to let the cells survive exposure to DNA damaging agents.
Figure 9 Proposed model for cAMP-mediated survival of DNA damage-induced BCP-ALL cells According to the model, cAMP-mediated survival of BCP-ALL cells exposed to DNA damage involves reduced p53-mediated apoptosis as well as p53-independent enhancement of autophagy. We suggest that autophagy needs to exceed a certain threshold in order to let the cells survive exposure to DNA damaging agents.CC BY · PubMed Central

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Authors
Skah, S., Richartz, N., Duthil, E., Gilljam, K. M., Bindesbøll, C., Naderi, E. H., Eriksen, A. B., Ruud, E., Dirdal, M. M., Simonsen, A., & Blomhoff, H. K.
Affiliations
  • Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
  • Department of Oncology, Section for Head and Neck Oncology, Oslo University Hospital, Oslo, Norway
  • Department of Hematology and Oncology, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Oslo, Norway
Venue
Oncotarget
Publisher
Impact Journals, LLC
Published
2018-07-13
Volume
9
Issue
54
Pages
30434-30449
ISSN
1949-2553
DOI
10.18632/oncotarget.25758
PMC
PMC6084393
Cited by
21
References
5
Language
en

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