Collagen‐Coated Poly(l‐lactide‐co‐ɛ‐caprolactone) Film: A Promising Scaffold for Cultured Periosteal Sheets
Abstract
BACKGROUND: We previously demonstrated that human periosteal sheets prepared on culture dishes function as an osteogenic "graft material" applicable to periodontal regenerative therapy. However, a lower level of initial adhesion of the excised periosteum tissue segments to culture dishes was a critical point that compromised the successful preparation of functional periosteal sheets. To improve on this weakness, we developed a transparent, biodegradable poly(L-lactide-co-ɛ-caprolactone) (LCL) film and tested its function as a scaffold and carrier of periosteal sheets. METHODS: Human periosteum tissue segments excised from alveolar bone of healthy donors were cultured on type I atelocollagen-coated LCL films. Initial adhesion was examined by simple agitation. Cell outgrowth and in vitro mineralization were cytohistochemically examined. Osteogenic activity was histochemically examined in an animal implantation model using nude mice. RESULTS: Surface collagen-coating modified the hydrophobic nature of LCL and substantially improved the initial adhesion. Compared to cultures in plastic dishes, the growth rate was delayed in non-coated films, but not in collagen-coated films. In the trimming process for animal implantation, periosteal sheets were frequently detached from non-coated films, but not from collagen-coated films. Regardless of collagen-coating, LCL films did not cause any significant infiltration of inflammatory cells, or negatively impact mineralized tissue formation. CONCLUSIONS: Collagen-coating improved the initial adhesion of periosteum segments, which facilitated cell outgrowth and also handling efficiency on implantation. Therefore, we believe that once evaluated in human studies, our collagen-coated LCL film will contribute to improving the periodontal regenerative methodology with the application of cultured autologous periosteal sheets.
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Coating collagen over other polymer scaffolds can enhance the adhesion of periosteum segments, which eases cell growth and improves the efficacy of the scaffold system.
“It was reported that the coating of collagen over the other polymer scaffolds can enhance the adhesion of periosteum segments which further eases the cell growth and improves the efficacy of the scaffold system [93].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [93]
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- Authors
- Kawase, T., Yamanaka, K., Suda, Y., Kaneko, T., Okuda, K., Kogami, H., Nakayama, H., Nagata, M., Wolff, L. F., & Yoshie, H.
- Affiliations
- Niigata University (JP)
- GC Corporation (Japan) (JP)
- Japan Science and Technology Agency (JP)
- University of Minnesota (US)
- Corresponding author
- Kawase, Tomoyuki
- Venue
- Journal of Periodontology
- Publisher
- Wiley
- Published
- 2010-11
- Volume
- 81
- Issue
- 11
- Pages
- 1653-1662
- ISSN
- 0022-3492 (ISSN-L)
- 1943-3670
- OpenAlex
- W2086893515
- Cited by
- 21
- References
- 14
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2012: 3
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Periodontology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- poly(L-lactide-co-ε-caprolactone), tissue engineering scaffolds, periodontal regeneration, osteogenic activity, in vitro mineralization, alveolar bone, autograft, hydrophobic surface modification
- MeSH terms
- Adolescent, Adult, Alveolar Process, Animals, Calcification, Physiologic, Cell Adhesion, Collagen, Elasticity, Female, Humans, Male, Materials Testing, Mice, Inbred BALB C, Mice, Nude, Osteogenesis, Periosteum, Polyesters, Surface Properties, Tensile Strength, Pliability, Coated Materials, Biocompatible, Absorbable Implants, Tissue Culture Techniques, Cell Proliferation, Mice, Tissue Scaffolds, Young Adult, Hydrophobic and Hydrophilic Interactions
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