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Recent Advances in β-glucosidase Engineering: A Brief Review
Open accessPreprint — published as 10.3390/molecules28134990 · Paper page
Abstract
β-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engineering in the field, including the efforts from our laboratory. We summarize and discuss the BGL engineering studies according to the targeted functions as well as the specific strategies used for BGL engineering.
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Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Metadata
- Authors
- Zhao, X., Huang, Y., Ouyang, B., Wang, G., Zhang, N., & Zuo, J.
- Affiliations
- Jiangxi Normal University (CN)
- Corresponding author
- Zhao, Xihua
- Huang, Yunhong
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2023-06-05
- Volume
- 28
- Issue
- 13
- Pages
- 4990-4990
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC10343665
- OpenAlex
- W4382060579
- Cited by
- 55
- References
- 1
- Citations by year
- 2026: 18 · 2025: 18 · 2024: 17 · 2023: 2
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Molecules (publishedVersion, cc-by)
- Preprints.org (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- University of Michigan
- National Natural Science Foundation of China (22068014, 21666010)
- Jiangxi Normal University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- β-glucosidase, enzyme engineering, lignocellulose degradation, enzyme stability, product inhibition, enzyme activity enhancement, industrial enzymes, sequence engineering, structural engineering
- MeSH terms
- beta-Glucosidase
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- The paper
- Zhao, X., Huang, Y., Ouyang, B., Wang, G., Zhang, N., & Zuo, J. (2023). Recent Advances in β-glucosidase Engineering: A Brief Review. Molecules. https://doi.org/10.20944/preprints202306.0235.v1
