Messenger RNA expression profiles and bioinformatics analysis of mouse hippocampi during exercise alleviates methamphetamine dependence via mRNA profile change in hippocampi
Open accessAbstract
Background: Methamphetamine (METH) is a highly addictive, psychoactive drug which can harm individual health and lead to great social problems. Various approaches have been adopted to address the problems arising from METH addiction, but relapse rates remain high. Recently, it has been found that comprehensive treatment combined with scientific and appropriate exercise interventions can improve the mental state and physical fitness of drug addicts and promote their physical and mental rehabilitation. Long-term, regular exercise improves the symptoms of METH withdrawal and reduces METH relapse. This study aimed to investigate the effects and regulated gene expression related to running exercise in METH-addicted mice. Methods: Male C57BL/6J mice were used to construct a METH addiction model. We performed a running exercise intervention and used conditioned place preference (CPP) to measure the effects of the running intervention on the METH-addicted mice. We also performed RNA sequencing (RNA-seq) and transcriptome analysis on the mice hippocampi, and the functions and differentially expressed genes (DEGs) that were significantly regulated by exercise intervention in the METH-addicted mice were analyzed and noted. Results: The results showed that days of CPP were shortened to 3 days in METH-addicted mice that underwent moderate exercise intervention, compared to 6 days in METH-addicted mice that went without exercise intervention. In addition, hippocampal transcriptome analysis revealed 12 DEGs significantly regulated by exercise intervention. By performing Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, it was revealed that the function of immune responses was significantly enriched in the METH-addicted mice undertaking exercise. The expression of 12 DEGs was verified by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), which showed that relative messenger RNA (mRNA) expression of DEGs was consistent with the RNA-seq results. Conclusions: A running intervention can promote the recovery of METH addiction in mice, and the 12 candidate DEGs from the mouse hippocampus can be used for further research on the regulatory mechanisms of exercise in METH-addicted mice.
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The Discussion addresses neurobiological mechanisms and potential treatments for methamphetamine dependence, not loneliness. Searches for loneliness, its neurobiological underpinnings, and social isolation found no passage addressing the attributed claim; the indexed passages provide no page numbers. (LLM, conf=0.76)
Experimental — accuracy not yet measuredThe brain-disease model can reduce blame but may also decrease hope for recovery and increase perceptions of dangerousness.
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From Beyond Stigma–Integrating Neurobiology, Genetics, and Awareness Integration Theory in Addiction Recovery (2026) · cites this paper as [82]
The Introduction and Discussion address methamphetamine dependence, its treatment, and exercise-related brain changes, but not public perceptions of a brain-disease model. Searches for blame, hope for recovery, and dangerousness found no passage addressing the attributed claim. (LLM, conf=0.76)
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“82”
- Authors
- Li, Y., Re, G.-F., Zhao, Y., Kong, D., Mao, J.-H., Wang, K.-H., & Kuang, Y.-Q.
- Affiliations
- Kunming Medical University (CN)
- Corresponding author
- Kuang, Yi-Qun
- Venue
- Annals of Translational Medicine
- Publisher
- AME Publishing Company
- Published
- 2022-09
- Volume
- 10
- Issue
- 18
- Pages
- 957-957
- ISSN
- 2305-5839 (ISSN-L)
- 2305-5847
- PMC
- PMC9577721
- OpenAlex
- W4292683008
- Cited by
- 6
- References
- 39
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 3 · 2022: 1
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Annals of Translational Medicine (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81870458, 2019PT310003, 2019ZF012, 202002AA100007)
- Kunming Medical University (2019ZF012, 2018HC005)
- Yunnan Provincial Department of Education (2019ZF012)
- Yunnan Provincial Science and Technology Department (2018HC005, 202002AA100007)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Health Sciences › Medicine › Epidemiology › Substance Abuse Treatment and Outcomes
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Keywords
- methamphetamine dependence, exercise intervention, running, hippocampus, transcriptome, differentially expressed genes, conditioned place preference, drug relapse, immune response, addiction recovery, mouse model
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