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PreprintCited by 6Bacterial Genetics and BiotechnologyVibrio bacteria research studiesAntibiotic Resistance in Bacteria

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The carRS-ompV-virK operon of Vibrio cholerae senses antimicrobial peptides and activates the expression of multiple resistance systems

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Mathieu-Denoncourt, Annabelle, Whitfield, Gregory B., Vincent, Antony T., Berne, Cécile, Pauzé-Foixet, Julien, Mahieddine, Feriel C., Brun, Yves V., Duperthuy, Marylise·2025·Scientific Reports
DOI 10.21203/rs.3.rs-5220433/v1PMC PMC12012098

Preprint — published as 10.1038/s41598-025-98217-3

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Abstract

Antimicrobial peptides are small cationic molecules produced by eukaryotic cells to combat infection, as well as by bacteria for niche competition. Polymyxin B (PmB), a cyclic antimicrobial peptide, is used prophylactically in livestock and as a last-resort treatment for multidrug-resistant bacterial infections in humans. In this study, a transcriptomic analysis in Vibrio cholerae showed that expression of the uncharacterized gene ompV is stimulated in response to PmB. We found that ompV is organized in a conserved four-gene operon with the two-component system carRS and virK in V. cholerae. A virK deletion mutant and an ompV deletion mutant were more sensitive to antimicrobials, suggesting that both OmpV and VirK contribute to antimicrobial resistance. Our transcriptomic analysis showed that the efflux pump vexAB, a known effector of PmB resistance, was upregulated in an ompV-dependent manner in the presence of PmB. The predicted structure of OmpV revealed a lateral opening in the β-barrel wall with access to an electronegative pocket in the barrel lumen that can accommodate PmB. Such an interaction could facilitate intracellular signaling through a conformational change in OmpV. This provides the first evidence of a specialized operon governing multiple systems for antimicrobial resistance in V. cholerae.

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Metadata

Authors
Mathieu-Denoncourt, A., Whitfield, G. B., Vincent, A. T., Berne, C., Pauzé-Foixet, J., Mahieddine, F. C., Brun, Y. V., & Duperthuy, M.
Affiliations
  • Université de Montréal
  • Université de Montréal (CA)
  • Université Laval (CA)
Corresponding author
Duperthuy, Marylise
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2025-03-25
Volume
15
Issue
1
Pages
13686-13686
ISSN
2045-2322 (ISSN-L)
DOI
10.21203/rs.3.rs-5220433/v1
PMC
PMC12012098
OpenAlex
W4409619677
Cited by
6
References
1
Citations by year
2026: 2 · 2025: 4
FWCI
3.50
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by-nc-nd)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Umeå Universitet
  • Canadian Institutes of Health Research (169053_1)
  • Natural Sciences and Engineering Research Council of Canada (RGPIN 2017 ?, BESCD3, rgpin-2017-05322)
  • Fonds de recherche du Québec – Nature et technologies
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
Keywords
Vibrio cholerae, antimicrobial peptides, polymyxin B, antimicrobial resistance, two-component systems, operon regulation, beta-barrel membrane proteins, multidrug resistance, transcriptomics
MeSH terms
Antimicrobial Peptides, Anti-Bacterial Agents, Bacterial Outer Membrane Proteins, Bacterial Proteins, Operon, Polymyxin B, Vibrio cholerae, Gene Expression Regulation, Bacterial, Gene Expression Profiling, Drug Resistance, Multiple, Bacterial

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