Somaclonal variation in habanero pepper (Capsicum chinense Jacq.) as assessed ISSR molecular markers
Open accessAbstract
Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper ( Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embryogenic callus system. A cluster analysis was performed to express in the form of dendrogram the relationships among different regeneration systems and the genetic variability detected. Genetic distance analysis indicated that our regeneration protocols are inappropriate for micropropagation, conservation, or genetic transformation; however, they may be applicable to breeding. This is the first report on the use of molecular analysis to evaluate genetic variation of in vitro-regenerated plants of Habanero pepper using ISSR markers.
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The most used DNA markers are ISSR, RAPD, RFLP, SSR, AFLP, and SCoT.
“The most used DNA markers are inter simple sequence repeats (ISSR), random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), microsatellites (SSR), amplified fragment length polymorphism (AFLP), and start codon-targeted (SCoT) polymorphism [47,48,49,50,51].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [50]
The Methods and Results and Discussion passages (page not provided) show that the paper uses ISSR markers and discusses RAPD and SSR markers used in other studies. They do not establish that the six listed markers are the most used, or address RFLP, AFLP, or SCoT. (LLM, conf=0.84)
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Research works printed but not linked: 4
- Kaeppler, S.M. and R.L. Phillips. 1993b. Tissue culture-induced DNA methylation variation in maize. Proc. Natl. Acad. Sci. USA 90:8773- 8776.
- Miguel, C. and L. Marum. 2011. An epigenetic view of plant cells cultured in vitro: Somaclo- nal variation and beyond. J. Expt. Bot. doi: 10.1093/jxb/err155. · DOI · Paper page
- Phillips, R.L., S.M. Kaeppler, and P. Olhoft. 1994. Genetic instability of plant tissue cultures: Breakdown of normal controls. Proc. Natl. Acad. Sci. USA 91:5222-5226.
- Stroud, H., B. Ding, S.A. Simon, S. Feng, M. Bellizzi, M. Pellegrini, G.L. Wang, B.C. Meyers, and S.E. Jacobsen. 2013. Plants regen- erated from tissue culture contain stable epi- genome changes in rice. eLIFE, <http://dx.doi. org/10.7554/eLife.00354>. · DOI
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Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M., & Sadh, R. K. (2016) · 3 Biotech · doi:10.1007/s13205-016-0389-7 · Cited by 436 · see citing works
Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
Application of Somaclonal Variation in Crop Improvements
Dorani, E., Dehghanian, Z., Gougerdchi, V., & Hamedpour-Darabi, M. (2024) · Sustainable Landscape Planning and Natural Resources Management · doi:10.1007/978-3-031-50729-8_8 · Cited by 11 · see citing works
- Authors
- Bello-Bello, J. J., Iglesias-Andreu, L. G., Avilés-Viñas, S. A., Gómez-Uc, E., Canto-Flick, A., & Santana-Buzzy, N.
- Affiliations
- Universidad Veracruzana (MX)
- Venue
- HortScience
- Published
- 2014-04
- Volume
- 49
- Issue
- 4
- Pages
- 481-485
- ISSN
- 0018-5345 (ISSN-L)
- 2327-9834
- OpenAlex
- W2473949921
- Cited by
- 24
- References
- 0
- Citations by year
- 2025: 5 · 2024: 3 · 2023: 4 · 2021: 4 · 2020: 3 · 2019: 3 · 2017: 1 · 2016: 1
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- HortScience (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- somaclonal variation, habanero pepper, Capsicum chinense, ISSR markers, in vitro regeneration, somatic embryos, embryogenic callus, micropropagation, genetic variation, plant breeding
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