Cited by 438Plant tissue culture and regenerationPlant Genetic and Mutation StudiesBanana Cultivation and Research

Somaclonal variations and their applications in horticultural crops improvement

Open access
Krishna, Hare, Alizadeh, Mahdi, Singh, Dhurendra, Singh, Udayvir, Chauhan, Nitesh, Eftekhari, Maliheh, Sadh, Radha Kishan·2016·3 Biotech

Abstract

The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s13205-016-0389-7

Cites

205 of 205 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Not all genetic variations that appear in somaclones of in vitro-regenerated plants have a phenotypic expression, mainly because these modifications either are not in coding sequences or do not alter the gene product to such an extent that can be observed in the phenotype.

    “In the case of in vitro-regenerated plants, not all the genetic variations that appear in the somaclones have a phenotypic expression, mainly because these modifications either are not in the coding sequences or do not alter the gene product to such an extent that can be observed in the phenotype [16].”

    From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [16]

    Split into 2 atoms — 0 confirmed, 2 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.

    Experimental — accuracy not yet measured
  • Somaclonal variations can appear in regenerants due to somatic mutations in the donor plant, such as chimeras in the explants.

    “Sometimes, due to somatic mutations in the donor plant (that is, due to the chimeras in the explants), the appearance of somaclonal variations can be observed in the regenerants [16].”
  • Most molecular techniques for detecting genetic variations between source plants and somaclones are based on DNA markers, because these markers are phenotypically neutral and are not influenced by developmental changes or environmental factors.

    “Most of these techniques are based on different types of DNA markers, due to the fact that these markers are phenotypically neutral and are not influenced by any developmental changes or environmental factors [16].”
  • Detection of genetic uniformity of in vitro-raised plants at an early stage is desirable.

    “Therefore, the detection of genetic uniformity of in vitro-raised plants at an early stage is desirable [22].”
  • Plant genotypes significantly influence somaclonal variation, as their susceptibility varies.

    “Notably, plant genotypes significantly influence this process, as their susceptibility to somaclonal variation varies [65,66].”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

2 of the 207 references couldn’t be matched to a known paper.

Research works printed but not linked: 8

  1. Battistini C Rosati P Dale A Lubby D In vitro evaluation of somaclonal strawberry (Fragaria × ananassa ‘Brighton’) variants for susceptibility to Phytophthora cactorum The strawberry into the 21st century 1991 Portland Timber Press 121 123
  2. Ben-Hayyim G Goffer Y Plantlet regeneration from NaCl-selected salt-tolerant callus culture of Shamouti oranges (Citrussinesis L. Osbeck) Plant Cell Rep 1989 7 680 683 24240461
  3. Grosser JW, Gmitter FG Jr, Chandler JL (1996) Somaclonal variation in sweet orange—a tool for cultivar improvement. In: Proceedings of International Society of Citriculture VIII Congress, Sun City, South Africa, pp 203–206
  4. Hwang S (2002) Somaclonal variational approach to breeding Cavendish banana for resistance to Fusarium wilt race 4. In: Global conference on banana and plantain, Bangalore, India, Abstract 57, October 28–31
  5. Mathur AK Akhila A Biotechnological studies in Cymbopogons Essential oil-bearing grasses: the genus Cymbopogons 2010 Boca Raton CRC Press, Taylor & Francis Group, LLC 107 133
  6. Mathur AK, Ahuja PS, Pandey B, Kukreja AK, Mathur A (1988) Development of superior strains of an aromatic grass—Cymbopogon winterianus Jowitt—through somaclonal variation. In: International conference on research in plant science and its relevance to future, Abstract, University Press, Delhi, p 160
  7. Morrison RA, Loh WHT, Green SK, Griggs TD, McLean BT (1989) Tissue culture of tomato and pepper: new tools for plant breeding. Tomato and pepper production in the tropics. In: Proceedings of international symposium on integrated management practices, Tainen, Taiwan. Shanhua, Taiwan, AVRDC, pp 44–50
  8. Toyoda H Horikoshi K Yamano Y Ouchi S Selection of Fusarium wilt disease resistance from regenerant derived from callus of strawberry Plant Cell Rep 1991 10 167 170 24221538

Cited by

Sorted by citations

Authors
Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M., & Sadh, R. K.
Affiliations
  • Indian Council of Agricultural Research (IN)
  • Gorgan University of Agricultural Sciences and Natural Resources (IR)
  • Central Institute for Arid Horticulture (IN)
Corresponding author
Krishna, Hare
Venue
3 Biotech
Published
2016-02-13
Volume
6
Issue
1
Pages
54-54
ISSN
  • 2190-572X (ISSN-L)
  • 2190-5738
OpenAlex
W2286740172
Cited by
436
References
207
Citations by year
2026: 28 · 2025: 40 · 2024: 40 · 2023: 56 · 2022: 60 · 2021: 64 · 2020: 52 · 2019: 35 · 2018: 39 · 2017: 19 · 2016: 3
FWCI
2.90
Type
journal article
Language
en
Open access
Locations
  • 3 Biotech (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Indian Agricultural Research Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Banana Cultivation and Research
Keywords
somaclonal variation, horticultural crops, micropropagation, genetic fidelity, in vitro regeneration, clonal propagation, germplasm conservation, epigenetic modifications, crop improvement, genetic variation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review