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Cited by 167

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Method for Discovering Novel DNA Viruses in Blood Using Viral Particle Selection and Shotgun Sequencing

Open access
Breitbart, Mya, Rohwer, Forest·2005·BioTechniques

Abstract

Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses combined with sequence-independent amplification and cloning. We show that both single- and double-stranded DNA viruses can be recovered from blood samples using this approach. In addition, we report the discovery of novel anellovirus sequences in the blood of healthy donors. PCR primers designed to amplify these novel anellovirus sequences were then used to verify the presence of these viruses in the general donor population.

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Crossref · 10.2144/000112019

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Metadata

Authors
Breitbart, M., & Rohwer, F.
Venue
BioTechniques
Published
2005-11
Volume
39
Issue
5
Pages
729-736
ISSN
  • 0736-6205 (ISSN-L)
  • 1940-9818
OpenAlex
W1974032924
Cited by
167
References
3
FWCI
1.61
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Method for Discovering Novel DNA Viruses in Blood Using Viral Particle Selection and Shotgun Sequencing. https://app.cochat.ai/papers/10.2144/000112019
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Breitbart, M., & Rohwer, F. (2005). Method for Discovering Novel DNA Viruses in Blood Using Viral Particle Selection and Shotgun Sequencing. BioTechniques. https://doi.org/10.2144/000112019

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.