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Mitochondrial Membrane Potential Probes and the Proton Gradient: A Practical Usage Guide
Open accessAbstract
Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.
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Cited by
Zielonka, J., Joseph, J., Sikora, A., Hardy, M., Ouari, O., Vasquez-Vivar, J., Cheng, G., Lopez, M., & Kalyanaraman, B. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00042 · Cited by 1,683
Sivandzade, F., Bhalerao, A., & Cucullo, L. (2019) · BIO-PROTOCOL · doi:10.21769/bioprotoc.3128 · Cited by 881
AUTACs: Cargo-Specific Degraders Using Selective Autophagy
Takahashi, D., Moriyama, J., Nakamura, T., Miki, E., Takahashi, E., Sato, A., Akaike, T., Itto-Nakama, K., & Arimoto, H. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.09.009 · Cited by 675
Increased demand for NAD(+) relative to ATP drives aerobic glycolysis
Luengo, A., Li, Z., Gui, D. Y., Sullivan, L. B., Zagorulya, M., Do, B. T., Ferreira, R., Naamati, A., Ali, A., Lewis, C. A., et al. (2021) · Molecular Cell · doi:10.1016/j.molcel.2020.12.012 · Cited by 547
The Microbiologist’s Guide to Membrane Potential Dynamics
Benarroch, J. M., & Asally, M. (2020) · Trends in Microbiology · doi:10.1016/j.tim.2019.12.008 · Cited by 333
Metadata
- Authors
- Perry, S. W., Norman, J. P., Barbieri, J., Brown, E. B., & Gelbard, H. A.
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 2011-02
- Volume
- 50
- Issue
- 2
- Pages
- 98-115
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W2091296592
- Cited by
- 1,187
- References
- 0
- FWCI
- 13.05
- Type
- journal article
- Language
- en
- Open access
- gold
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- Perry, S. W., Norman, J. P., Barbieri, J., Brown, E. B., & Gelbard, H. A. (2011). Mitochondrial Membrane Potential Probes and the Proton Gradient: A Practical Usage Guide. BioTechniques. https://doi.org/10.2144/000113610
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