Exercise-Induced Overexpression of Angiogenic Factors and Reduction of Ischemia / Reperfusion Injury in Stroke
Abstract
The purpose of this study was to determine if exercise could induce expression of vascular endothelial growth factor (VEGF) and angiopoietin 1 and 2, in association with angiogenesis; and if angiogenic changes correlated with reduced brain injury in stroke. Adult male Sprague Dawley rats (3 month old, n=44) were exercised on a treadmill 30 minutes each day for 1, 3 or 6 weeks, or housed as non-exercised controls for 3 weeks. Some 3 week-exercised rats were then housed for an additional 3 weeks. Exercise significantly (p<0.01) increased mRNA (determined by real-time reverse transcriptase-polymerase chain reaction) expression of angiopoietin 1 and 2 as early as 1 week, with further increases occurring at 3 weeks. A mild increase after 1 week and a robust increase after 3 weeks of exercise in four isoforms (120, 144, 164, 188) of VEGF mRNA levels were significantly (p<0.01) observed, with VEGF(144) being more markedly up-regulated. Overexpression of the mRNAs decreased upon withdrawal of exercise. A significant increase (p<0.01) in the density of microvessels (determined by laminin-immunocytochemistry) was found at 3 weeks of exercise and this continued after exercise was withdrawn. In exercising rats subjected to 2-h MCA occlusion followed by 48-h reperfusion, neurological deficits and infarct volume were significantly reduced. Neuroprotection continued after 3 weeks of rest. This study indicates that pre-ischemic exercise reduces brain injury in stroke. The reduced damage is a…
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Exercise increases production of IGF-1 and VEGF, which are linked to angiogenesis and neurogenesis.
“Other researchers have reported exercise-related increased productions of other neurotrophic factors such as insulin-like growth factor 1 and vascular endothelial growth factor, which have been linked with angiogenesis (the growth of new vasculature) and neurogenesis”
From Enrichment Effects on Adult Cognitive Development: Can the Functional Capacity of Older Adults Be Preserved and Enhanced? (2008) · cites this paper as Ding et al., 2004
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Chaddock, L., Erickson, K. I., Prakash, R. S., VanPatter, M., Voss, M. W., Pontifex, M. B., Raine, L. B., Hillman, C. H., & Kramer, A. F. (2010) · Developmental Neuroscience · doi:10.1159/000316648 · Cited by 353 · see citing works
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Villar-Cheda, B., Sousa-Ribeiro, D., Rodriguez-Pallares, J., Rodriguez-Perez, A. I., Guerra, M. J., & Labandeira-Garcia, J. L. (2009) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2008.127 · Cited by 59 · see citing works
- Authors
- Ding, Y.-H., Luan, X.-D., Li, J., Rafols, J., Guthinkonda, M., Diaz, F., & Ding, Y.
- Affiliations
- Wayne State University (US)
- ORCID
- Venue
- Current Neurovascular Research
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2004-12-01
- Volume
- 1
- Issue
- 5
- Pages
- 411-420
- ISSN
- 1567-2026 (ISSN-L)
- OpenAlex
- W2099992963
- Cited by
- 150
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 5 · 2022: 1 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 8 · 2017: 11 · 2016: 9 · 2015: 11 · 2014: 9 · 2013: 14 · 2012: 17
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Neurovascular Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service (09-59-00(M02)ER)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Rehabilitation › Stroke Rehabilitation and Recovery
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- physical activity, stroke, ischemia-reperfusion injury, angiogenesis, vascular endothelial growth factor, angiopoietin-1, angiopoietin-2, VEGF isoforms, microvessel density, neuroprotection, middle cerebral artery occlusion, infarct size, gene expression
- MeSH terms
- Stroke Rehabilitation, Analysis of Variance, Animals, Disease Models, Animal, Gene Expression Regulation, Immunohistochemistry, Laminin, Male, Neurologic Examination, Physical Conditioning, Animal, Random Allocation, Time Factors, Reperfusion Injury, Rats, Sprague-Dawley, Neovascularization, Physiologic, Brain Infarction, Stroke, Vascular Endothelial Growth Factor A, Angiogenesis Inducing Agents, Rats
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