Fast-twitch muscle hypertrophy partly induces lipid accumulation inhibition with Alaska pollack protein intake in rats
Open accessAbstract
Fish protein is a source of animal protein that is consumed worldwide. Although it has been reported that the intake of Alaska pollack protein (APP) reduces serum triglyceride and body fat accumulation in rats, the mechanisms underlying these effects are poorly understood. In the present study, we fed 5-week-old male Sprague-Dawley rats a high-fat diet with APP or casein for 4 weeks. We reconfirmed that the intake of APP decreases serum triglycerides and inhibits visceral body fat accumulation in rats. We found that APP had a higher non-digestive protein content than casein, and the amount of protein in feces was higher in the APP group than in the casein group. However, the amount of total lipids in feces did not differ significantly between the groups. We also found that the gastrocnemius muscle, a fast-twitch muscle, tended to increase in weight, and that the epididymal fat weight correlated negatively with gastrocnemius muscle weight in the APP group. These results imply that the enhancement of basal energy expenditure by fast-twitch muscle hypertrophy, rather than the enhancement of lipid excretion via feces, partly causes APP-induced inhibition of lipid accumulation in rats.
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Ingestions of APP for 6 or 8 weeks induce significant increases in the gastrocnemius muscle weight and fiber diameters when comparing with ingestion of CAS in rats.
“The previous study demonstrated that ingestions of APP for 6 or 8 weeks induce significant increases in the gastrocnemius muscle weight and fiber diameters when comparing with ingestion of CAS (14–16) and a significant increase of myosin heavy-chain gene expression in Myh4 (14) in rats.”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [15]
The Methods describe a four-week APP-versus-casein experiment, not six or eight weeks. The Results report only a trend toward greater gastrocnemius weight with APP (P = 0.074), contrary to the claimed significant increase; the Discussion says the study observed tissue-weight changes only, not fiber diameters. Page numbers are not available in our copy. (LLM, conf=0.90)
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Experimental — accuracy not yet measuredAlaska pollack protein (APP) ingestion induced more marked muscle hypertrophy compared with casein (CAS) in rat muscle.
“Mizushige and colleagues (14), Kawabata and colleagues (15), and Morisasa and colleagues (16) showed that Alaska pollack protein (APP) ingestion induced more marked muscle hypertrophy comparing with casein (CAS) in rat muscle (14–16).”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [15]
The Results (page not provided) report that gastrocnemius muscle weight tended to be higher in APP-fed rats than in casein-fed rats, but the difference was not statistically significant (P = 0.074). Soleus muscle weight did not differ. The claim overstates this muscle-specific trend as established hypertrophy in rat muscle generally. (LLM, conf=0.89)
Experimental — accuracy not yet measuredMizushige and colleagues (15) and Kawabata and colleagues (14) suggested that muscle hypertrophy induced by APP ingestion leads the enhancement of basal energy expenditure.
“Moreover, Mizushige and colleagues (15) and Kawabata and colleagues (14) suggested that muscle hypertrophy induced by APP ingestion leads the enhancement of basal energy expenditure.”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [15]
Mizushige and colleagues (15) showed decrease in serum triglycerides and inhibition of visceral body fat accumulation in rats.
“Also Mizushige and colleagues (15) showed decrease in serum triglycerides and inhibition of visceral body fat accumulation in rats, and Morisasa and colleagues reported that lipid molecules were significantly changed in APP-fed rats than in CAS-fed rats (16).”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [15]
The intake of fish protein (APP) induced more marked muscle hypertrophy compared with casein in rat muscle.
“the intake of fish protein, i.e., APP, which Japan and other countries have traditionally consumed in daily meals, induced more marked muscle hypertrophy comparing with casein in rat muscle (Mizushige et al., 2010; Kawabata et al., 2015).”
From Effect of resistance training and fish protein intake on motor unit firing pattern and motor function of elderly (2018) · cites this paper as Mizushige et al., 2010
Feeding 5-week-old male Sprague-Dawley rats a high-fat diet with Alaska pollack protein for four weeks resulted in higher weight of gastrocnemius muscle, which is rich in fast-twitch fiber, compared to casein-fed rats.
“feeding 5-week-old male Sprague-Dawley rats a high-fat diet with Alaska pollack protein (APP) for four weeks resulted in higher weight of gastrocnemius muscle, which is rich in fast-twitch fiber compared to casein-fed rats [57].”
From Novel Insights on Intake of Fish and Prevention of Sarcopenia: All Reasons for an Adequate Consumption (2020) · cites this paper as [57]
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References 17 · Cited by 35
- Authors
- Mizushige, T., Kawabata, F., Uozumi, K., Tsuji, T., Kishida, T., & Ebihara, K.
- Affiliations
- Ehime University (JP)
- Nippon Suisan Kaisha (Japan) (JP)
- Venue
- Biomedical Research
- Publisher
- Biomedical Research Press
- Published
- 2010
- Volume
- 31
- Issue
- 6
- Pages
- 347-352
- ISSN
- 0388-6107 (ISSN-L)
- 1880-313X
- OpenAlex
- W2012468927
- Cited by
- 35
- References
- 17
- Citations by year
- 2026: 1 · 2024: 7 · 2023: 4 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 1 · 2012: 2
- FWCI
- 0.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biomedical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
- Keywords
- fish protein, lipid accumulation, serum triglycerides, visceral fat, gastrocnemius, epididymal adipose tissue, fecal fat excretion, energy expenditure, high-fat diet, casein
- MeSH terms
- Animals, Bile Ducts, Body Composition, Energy Intake, Caseins, Dietary Fats, Dietary Proteins, Energy Metabolism, Hypertrophy, Male, Triglycerides, Rats, Sprague-Dawley, Muscle Fibers, Fast-Twitch, Fish Proteins, Gadiformes, Intra-Abdominal Fat, Lipid Metabolism, Rats
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